童和林,冯素萍,贺军虎,王静毅,陈业渊,孙光明,于飞,武耀廷

• 1:中国热带农业科学院热带作物生物技术研究所农业部热带作物生物技术重点开放实验室
• 2:海南大学农学院
• 3:琼州大学
• 4:中国热带农业科学院热带作物品种资源研究所

摘要(Abstract)：

初步构建了菠萝种质的SSR指纹图谱,为菠萝种质鉴定提供了依据。从菠萝EST-SSR开发的20对微卫星引物扩增31份不同的菠萝种质,筛选出能够稳定扩增且带型差异明显的SSR引物。挑选5对多态性高、分辨率高、重复性好的SSR引物对,作为标准引物对材料进行扩增。每对引物得到的多态性带数8～15个,平均为11条;引物的多态信息含量(PIC)在0.643 7～0.792 8,平均0.737 3。依据带型特征,建立菠萝DNA分子指纹图谱。

关键词(KeyWords)： 菠萝;SSR;指纹图谱

Abstract:

Keywords:

基金项目(Foundation): 国家科技基础条件平台重点项目(2005DKA21000-5-43)

作者(Author): 童和林,冯素萍,贺军虎,王静毅,陈业渊,孙光明,于飞,武耀廷

DOI: 10.13925/j.cnki.gsxb.2011.02.014

参考文献(References)：

• [1]Institute of Fruit Tree Research Guangdong Academy of AgriculturalSciences.Pineapple and Cultivation[M].Beijing:China Light Indus-try Press,1987:83-99.广东省农业科学院果树研究所.菠萝及其栽培[M].北京:轻工业出版社,1987:83-99.
• [2]WU Yao-ting,ZHANG Tian-zhen,GUO Wang-zhen,YIN Jian-mei.Detecting polymorphis-m among upland cotton.(Gossypi-umhirsutum L.)cultivars and their roles in seed purity of hybridswith SSR markers[J].Cotton Science,2001,13(3):131-133.武耀廷,张天真,郭旺珍,殷剑美.陆地棉品种SSR标记的多态性及用于杂交种纯度检测的研究[J].棉花学报,2001,13(3):131-133.
• [3]LIU Jie,LIU Gong-she,QI Dong-mei,LI Fang-fang.Constructionof genetic fingerprints of aneurolepidium chinensis Using mi-crosatellite sequences[J].Acta Botanica Sinica,2000,42(9):985-987.刘杰,刘公社,齐冬梅,李芳芳.用微卫星序列构建羊草遗传指纹图谱[J].植物学报,2000,42(9):985-987.
• [4]TAN Jun,DING Zhong-fang,SUN Shi-xian,LIN Yong,TANG Hai-tao,HUANG Yu-bi,YANG Jun-pin.SSR fingerprints of maize inbred-lines widely used in southwest China[J].Southwest China J ofAgr Sci,2003,16(2):1-6.谭君,丁仲芳,孙仕贤,林勇,唐海涛,黄玉碧,杨俊品.西南常用玉米自交系SSR指纹图谱构建[J].西南农业学报,2003,16(2):1-6.
• [5]LI Jin-bo,FANG Xuan-jun,YANG Guo-cai,FEI Zhen-jiang,QIHua-xiong.SSR fingerprin-ting the parents of two-line hybrid riceand its application in purity identification of hybrid seeds[J].HybridRice,2005,20(2):50-53.李进波,方宣钧,杨国才,费震江,戚华雄.两系杂交稻亲本SSR指纹图谱的建立及其在种子纯度鉴定中的应用[J].杂交水稻,2005,20(2):50-53.
• [6]SONG Q J,AKKAYA M S,BHAGWAT A A.A selected set of trin-ucleotide simple sequence repeat markers for soy bean cultivar iden-tification.special issue:cultivar group classification[J].Plant Vari-eties and Seeds,1999,12(3):207-220.
• [7]RODER M S,WENDEHAKE K,KORZUN V,BREDEMEIJER G,LABORIE D,BERTRAND L,ISAAC P,REN-RELL S,JACKSONJ,COOKE R J,VOSMAN B,GANAL M W.Construction and anal-ysis of a microsate-llite-based database of European wheat varieties[J].Theor Appl Genet,2002,106:67-73.
• [8]LIU Wei-guo,YI Gan-jun,LIU yan,ZHANG Qiu-ming,ZENG Ji-wu.Identification of pine apple germplasm and relationship analysiswith AFLP[J].Journal of Fruit Science,2008,25(4):516-520.刘卫国,易干军,刘岩,张秋明,曾继吾.菠萝种质鉴定及亲缘关系的AFLP分析[J].果树学报,2008,25(4):516-520.
• [9]ZHANG Ru-lian,FU Xiao-xia,QI Zhi-ping,CHEN Ye-yuan.IS-SR Analysis on 17 Pineapple Resources[J].Chinese AgriculturalScience Bulletin,2006,22(6):428-431.张如莲,傅小霞,漆智平,陈业渊.菠萝17份种质的ISSR分析[J].热带农业科学,2006,22(6):428-431.
• [10]FEUSER S,MELER K,DAPUINTA M.Genotypic fidelity of micro-propagated pineapple(Ananas comosus)plantlets assessed byisozyme and RAPD markers[J].Plant Cell,Tissue and Organ Culture,2003,72:221-227.
• [11]DUVAL M F,NOYER J L,PERRIER X,EECKENBRUGGE G C,HAMON P.Molecular diversity in pineapple assessed by RFLPmarkers[J].Theor Appl Genet,2001,102:83-90.
• [12]POREBSKI S,BAILEY L G,BAUM B R.Modification of a CTABDNA extraction protocol for plants containing high polysaccharideand polyphenol components[J].Plant Molecular Biology Reporter,1997,15(1):8-15.
• [13]WANG Li-xin,ZHAO Chang-ping,QIU Jun,LI Hong-bo,GE Ling-ling,SUN Hui,YAO Ji.A new scoring method of SSR patterns forwheat[J].Journal of Triticeae Crops,2006,26(4):164-168.王立新,赵昌平,邱军,李宏博,葛玲玲,孙辉,姚骥.记录小麦SSR带型的快捷方法[J].麦类作物学报,2006,26(4):164-168.
• [14]WANG Jing-yi,CHEN Ye-yuan,HUANG Bing-zhi,YU Fei,WUYao-ting.Establishment of fingerprinting for bananas(Musa nana)by SSR marker[J].Journal of Fruit Science,2009,26(5):733-738.王静毅,陈业渊,黄秉智,于飞,武耀廷.部分香蕉品种SSR指纹图谱的构建[J].果树学报,2009,26(5):733-738.
• [15]FEUSER S,MELER K,DAPUINTA M.Genotypic fidelity of micro-propagated pineapple(Ananas comosus)plantlets assessed by isozymeand RAPD markers[J].Plant Cell,Tissue and Organ Culture,2003,72:221-227.
• [16]LI Xin-hai,FU Jun-hua,ZHANG Shi-huang,YUAN Li-xing,LIMing-shun.Genetic variation of inbred lines of maize detected bySSR markers[J].Scientia Agricultura Sinica,2000,33(2):1-9.李新海,傅骏骅,张世煌,袁力行,李明顺.利用SSR标记研究玉米自交系的遗传变异[J].中国农业科学,2000,33(2):1-9.