香蕉腺苷酸核糖基化作用因子1(MaArf1)基因的克隆及序列结构分析Cloning and structural characterization of ADP-ribosylation-factor 1 in banana (Musa accuminata L. AAA)
王园;王甲水;张建斌;徐碧玉;
摘要(Abstract)：
Arf(ADP-ribosylation factors)作为小G蛋白家族中的一类,在植物细胞的胞内运输中起着重要的作用。从采后2d的香蕉(Musa accuminata L.AAA group)果实cDNA文库中筛选到一条Arf基因,命名为MaArf1。序列分析表明,MaArf1在植物中是高度保守的。与水稻、苜蓿、马铃薯、小麦和拟南芥等物种中Arf基因的相似性都超过95%。并且MaArf1分别在24-31(序列为GLDAAGKT,Arf蛋白P结构域),67-71(序列为DVGGQ,G'结构域),126-130(序列为NKQDL,G结构域)的氨基酸处也具有保守的GTP结合结构域。根据已获得的cDNA序列设计引物,研究又获得了该基因的基因组序列。对其结构分析发现,该序列全长1998bp,由5个外显子和4个内含子组成。内含子序列中存在大量的重复序列,并且在第4个内含子中有一个完整的开放阅读框,推测这些序列可能参与基因的表达调控,也可能与RNA水平上的可变剪接有关。
关键词(KeyWords)： 香蕉(Musaaccuminata L.AAAgroup);MaArf1;cDNA;基因组序列
基金项目(Foundation): 公益性行业(农业)科研专项(nyhyzx07-029);; 现代农业产业技术体系建设专项资金(nycytx-33);; 热带生物技术研究所中央级公益性基本科研业务费(ITBBZB0734)
作者(Author): 王园;王甲水;张建斌;徐碧玉;
Email:
DOI: 10.13925/j.cnki.gsxb.2010.03.013
参考文献(References)：
- [1]YANG Z.Small GTPases:versatile signaling switches in plants[J].Plant Cell,2002,14:375-388.
- [2]VERNOUD V,HORTON A C,YANG Z,NIELSEN E,Analysis of the small GTPase gene superfamily of Arabidopsis[J].Plant Physiol,2003,13(1):1191-1208.
- [3]TAKAI Y,SASAKI T,MATOZAKI T.Small GTP-binding proteins[J].Physiolgy Rev,2001,81:153-208.
- [4]BALCH W E,KAHN R A,SCHWANINGER R.ADP-ribosylation factor required for vesicular trafficking between the endoplasmic reticulum and the cis-Golgi compartment[J].Journal of Biological Chemistry,1992,26(7):13053-13061.
- [5]MOSS J,VAUGHAN M.Molecules in the ARF orbit[J].Journal of Biological Chemistry,1998,27(3):21431-21434.
- [6]CHONG K,ZHUANG X L.ARF-GTPase as a Molecular switch for polar auxin transport mediated by vesicle trafficking in root develop-ment[J].Plant Signaling&Behavior,2007,2(2):101-102.
- [7]XU B Y,SU W,LIU J H,WANG J B,JIN Z Q.Differentially ex-pressed cDNAs at the early stage of banana ripening identified by suppression subtractive hybridization and cDNA microarray[J].Plan-ta,2007,226:529-539.
- [8]WAN C Y,WILKINS T A.A modified hot borate method signifi-cantly enhance the yield of high-quality RNA from cotton(Gossypi-um hirsutum L.)[J].Anal Biochem,1994,223:7-12.
- [9]HUANG J,GE X,SUN M.Modified CTAB protocol using a silica matrix for isolation of plant genomic DNA[J].Biotechnique,2000,28:432-434.
- [10]YOUSSEFIAN S,NAKAMURA M,SANO H.Molecular characteri-zation of rgp2,a gene encoding a small GTP-binding protein from rice[J].Molecular and General Genetics,1993,237:187-192.
- [11]REGAD F,BARDET C,TREMOUSATGUE D,MOISAN A,LES-CURE B,AXEFOS M.cDNA cloning and expression of an Ara-bidopsis GTP-binding protein of the ARF family[J].FFBS Lett,1993,31(6):133-136.
- [12]SZOPA J,MULLER R B.Cloning and expression analysis of an ADP-ribosylation factor from Solanum tubeeosum[J].Plant Cell Rep,1994,14:180-183.
- [13]UEHARA A K,ETSUO S,HANDA H.Cloning and expression analy-ses of cDNA encoding an ADP-ribosylation factor from wheat:tis-sue-specific expression of wheat ARF[J].Plant Science,2001,160:535-542.
- [14]KIYOSUE T,SHINOZAKI K.Cloning of a carrot cDNA for a member of the family of ADP-ribosylation factors(ARFs)and characteriza-tion of binding of nucleotides by its product after expression in E.coli[J].Plant Cell Physiology,1995,36:849-856.
- [15]YUKI A,ERIKO I,RYUJI S,FUMIYA K.Cloning and expression of cDNAs encoding ADP-ribosylation factor in carrot seedling[J].Plant Science,2007,17(2):189-195.
- [16]REN Mao-zhi,CHEN Quan-jia,ZHANG Rui,GUO San-dui.The structural characteristics,alternative splicing and genetic experes-sion analysis of ADP-ribosylation factor1(arf1)in cotton[J].Acta Genetica Sinica,2004,31(8):850-857.任茂智,陈全家,张锐,郭三堆.棉花腺苷酸核糖基化作用因子1(A rf1)的结构特征、替换剪接和遗传表达分析[J].遗传学报,2004,31(8):850-857.
- [17]SHARP P A.Split genes and RNA splicing[J].Cell,1994,77:805-815.