酿酒葡萄原生质体再生植株Plant Regeneration From Protoplasts of Wine-making Grapevine
于向荣,李佩芬,卢炳芝,王敏,郑旭琴,刘延松
摘要(Abstract):
将酿酒葡萄品种白诗南、梅郁的花丝接种在含6BA2.0mg·1-1、2,4-D0.5mg·1-1的B5诱导培养基上,诱导产生胚性愈伤组织。胚性愈伤组织经液体悬浮培养形成含大量胚性细胞团的悬浮培养物。用Cellulase Rs2%、Pectolyase Y230.3%,5mmol·1-1CaCl2·2H2O、0.6mol·1-1甘露醇、0.3%葡聚糖硫酸钾、pH5.6~5.8的酶混合液从胚性细胞团分离得到原生质体。原生质体培养到第5天出现第一次分裂,40d形成上百个细胞的大细胞团,50d形成0.5~1.0mm大小的小愈伤组织。这些小愈伤组织在含6BA0.5mg·1-1的B5分化培养基上分化出胚状体进而形成幼苗,在生根培养基上生根形成再生植株。
关键词(KeyWords): 酿酒葡萄;原生质体;再生植株
基金项目(Foundation):
作者(Author): 于向荣,李佩芬,卢炳芝,王敏,郑旭琴,刘延松
DOI: 10.13925/j.cnki.gsxb.1999.02.007
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