刘菊华;徐碧玉;陈敦忠;张静;张建斌;贾彩红;金志强;

• 1:中国热带农业科学院热带生物技术研究所/农业部热带生物技术重点开放实验室
• 2:中国热带农业科学院海口试验站

摘要(Abstract)：

采用筛选香蕉果实采后cDNA文库的方法,首次获得了一条长为1 341 bp的cDNA序列,命名为MaEF1A。生物信息学分析结果表明,它与麝香百合延长因子1A 3的mRNA有84%的同源性,与烟草延长因子1A的mRNA有83%的同源性,推测它可能为编码香蕉延长因子1A(elongation factor-1 alpha,EF1A)的基因。该基因包含了一个完整的ORF,肽链序列中含有GTP-EFTU、GTP-EFTU-02和GTP-EFTU-03 3个保守结构域。采用RT-PCR的方法对该基因在香蕉不同组织以及果实采后不同时期的表达特征进行了初步的研究。结果表明,该基因在香蕉不同组织以及果实采后各个时期都是差异表达的。该基因在香蕉根和花中的表达量最高,茎叶最低。在香蕉果实采后的表达量开始是呈逐渐升高的趋势,到香蕉果实采后第20天,该基因的表达量达到高峰,随后该基因的表达量就逐渐下降。这一变化过程与香蕉果实采后乙烯释放量的变化进程是一致的。

关键词(KeyWords)： 香蕉;cDNA文库;MaEF1A;RT-PCR

Abstract:

Keywords:

基金项目(Foundation): 现代农业产业技术体系建设专项(nycytx-33);; 公益性行业(农业)科研专项(nyhyzx07-029);; 热带生物技术研究所中央级公益性科研院所基本科研业务费专项(ITBB110202)

作者(Authors): 刘菊华;徐碧玉;陈敦忠;张静;张建斌;贾彩红;金志强;

DOI: 10.13925/j.cnki.gsxb.2011.05.018

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