冯超;朱长青;徐昌杰;陈昆松;

• 1:浙江大学果实品质生物学实验室·农业部园艺作物生长发育与品质生物学实验室

摘要(Abstract)：

RNA-Seq为植物功能基因组学研究的重要手段,近年来在果树上也有不少成功应用。应用这一技术,可获得高通量的转录本序列信息及表达量数据。本文首先简要介绍RNA-Seq研究背景及其在果实发育与品质形成、芽发育及其调控、果树胁迫响应等方面的应用概况,然后着重分物种追踪了柑橘、葡萄、香蕉、草莓、苹果、梨、桃、樱桃、杨梅、蓝莓这10个常见果树的30余个RNA-Seq实例,介绍了具体应用进展。最后就存在的测序成本和数据处理技术等问题以及测序平台和文库制备等方面的发展趋势进行了讨论。

关键词(KeyWords)： 果树;第2代测序技术;RNA-Seq;转录组学;果实发育;果实成熟;胁迫

Abstract:

Keywords:

基金项目(Foundation): 863项目(2013AA102606);; 国家自然科学基金(31030052,31071781);; 国家公益性行业(农业)科研专项(200903044,201203089)

作者(Author): 冯超;朱长青;徐昌杰;陈昆松;

Email:

DOI: 10.13925/j.cnki.gsxb.2014.01.019

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