马丽;侯乐峰;郝兆祥;马耀华;周玉亮;明东风;

• 1:枣庄学院生命科学学院
• 2:枣庄市石榴研究中心

摘要(Abstract)：

【目的】研究不同性状石榴品种的遗传关系和遗传多样性,为石榴种质资源分类、品种鉴定和有效利用提供参考依据。【方法】以具有不同性状的82个石榴品种为材料,用ISSR标记分析石榴种质资源的遗传多样性和品种亲缘关系。【结果】用筛选出的10条扩增清晰、多态性较好的引物对82个品种进行PCR扩增,共扩增出186个位点,其中多态性位点178个,多态性位点百分率(PPB)为95.69%,平均等位基因数(Na)为1.957 0,平均有效等位基因数(Ne)为1.302 4,平均Nei's的基因多样性指数(He)为0.214 0,平均Shannon信息指数(I)为0.356 7,品种间遗传相似系数(Gs)为0.478 4⁰.994 6,UPGMA聚类结果在Gs为0.735时,将82个品种分为4大类。遗传相似系数矩阵和Cophenetic值之间的相关系数为0.985,说明聚类结果较准确。【结论】利用ISSR标记技术可有效揭示石榴种质资源的遗传多样性和品种间亲缘关系,82个石榴品种遗传多样性丰富。ISSR聚类结果与根据花瓣类型、结实性、籽粒硬度性状的分类结果吻合度较高,与地理生态环境也有一定的相关性。研究结果对石榴种质资源的保存、分类鉴定及杂交育种有重要参考意义。

关键词(KeyWords)： 石榴;ISSR;遗传多样性;亲缘关系

Abstract:

Keywords:

基金项目(Foundation): 国家林业公益性行业科研专项(编号:201204402);; 国家林业局林木种苗工程项目:山东省枣庄市峄城区中国石榴种质资源圃建设项目(编号:[2012]1888号);; 山东省高等学校科技计划项目(编号:J14LF02)

作者(Authors): 马丽;侯乐峰;郝兆祥;马耀华;周玉亮;明东风;

DOI: 10.13925/j.cnki.gsxb.20150008

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