王乔春

• 1:四川农业大学园艺系 雅安625014

摘要(Abstract)：

BAP、IBA和GA_3对梨砧木BP10030茎尖外殖体的成活率没有明显影响,但0.1mg·1~(-1)GA_3明显促进外殖体的初期生长。外殖体建立阶段以1mg·1~(-1)BAP+0.1mg·1~(-1)IBA+0.1mg·1~(-1)GA_3的处理为宜。BAP与IBA明显影响茎的增殖。茎数量随BAP浓度(1—4mg·1~(-1))的增加而增加,但茎长度明显下降。在BAP存在条件下,增加0.1mg·1~(-1)IBA对茎数量没有明显影响,但显著促进茎增长。高浓度的BAP(4rug·1~(-1))明显导致玻璃苗产生,增施0.1mg·1~(-1)IBA在一定程度上可抑制玻璃苗形成。茎增殖阶段BAP浓度明显影响试管苗生根,其生根率、平均根数与限长度随BAP浓度的增加(1—4rug·1~(-1))而降低。2mg·1~(-1)BAP+0.1mg·1~(-1)IBA为茎增殖阶段的最适浓度。生根阶段2mg·1~(-1)IBA显著提高生根率、根数与根长度,同时也有利于试管苗移栽,促进试管苗初期生长。

关键词(KeyWords)： 植物生长调节剂;梨;试管苗;繁殖

Abstract:

Keywords:

基金项目(Foundation): 本研究在瑞典农业大学园艺植物育钟研究所完成,受到瑞典SI科学基金会资助

作者(Author): 王乔春

DOI: 10.13925/j.cnki.gsxb.1995.01.004

参考文献(References)：

• 1 Sangwan-Norreel B, Dubois F, Flandre F et al. In vitro culture and plant improvement. Acta Hort, 1991,289:19—32
• 2 George E F and Sherrington P D. Plant propagation by tissue culture. Exegetics Ltd, 1984
• 3 Skoog F and Miller C O. Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp Soc Exptl Biol, 1957,11:118—130
• 4 Dunatan D I. Turner K and Lazaroffr W R. Propagation in vitro of the apple rootstock M4:effect of phytohormones on shoot quality. Plant Cell Tissue Organ Culture, 1985,4:55—60
• 5 Paques M. Vitrification and micropro pagation: causes, remedies and prospects. Acta Hort, 1991, 289:283—290
• 6 Wainwright H. Problems in rooting cultured shoots, in:Micropropagation in Horticulture: Practice and commercial problems (ed P. G. Alderson and W.M. Dullforce). 1986. 161—172
• 7 王乔春．梨试管苗的生根．果树科学,1994,11(3):145—148
• 8 Quoirin M, Lepoivre Ph and Boxus Ph. Unpremierbilan de Ioann'ees de recherches sur les cultures de meristemes et la multiplication in vitro de fruitier ligneux. C. R. des Recherches 1976—1977. Station des cultures fruitieres et maricheres a Gembloux, 1977.93—117
• 9 Bhojwani S. Mullins K and Cohen D. In vitro Propagagtion of Pyrus pyrifolia. Sci Hort, 1984.23:247—254
• 10 Lane D. Regeneration of pear plants from shoot meristem tips. Plant Sci Lett. 1979,13:181-285
• 11 Shes X-Sh and Mullins M G. Propagation in vitro of pear. Pyrus communis L., cuhivars "William's Bon chretien". "Packham's Triumph" and "Beurre Bose". Sci Hort, 1984, 23:51—57
• l2 Viseur J. Micropropagation of pear, Pyrus rommuniS L., in a doubIe-pha.e culture edium. Art
• l3 Maarri K A, Arnaud Y and Miginiac E. In vitro micropropagation of Quince (Cydonin oblonga mill. )
• 28:315-321.
• l4 Masuda T, Yoshida Y, Bessho H et al. Studies on cell, tissue and shoot-tip culture ih apple. I.
• abnormal shoot formation and plant homones. BulI Tree Res Stn, l988, l5:2l--28
• l5 Phan C T and Letouze R. A comparative study of cholorophyll, phanolic and protein contets and of
• CoA ligase activity of normal and vitreous plants (Prunus avium L. ) obtained in vitro. Plan Sci
• - 327
• l6 Rugini E, Tarini P and Rossodivita M E. Control of shoot vitrification of almed and olive grown
• Hort, 1987,212: l7- 183
• 17 Vieitel. A M, Ballester A, san-Jose M C et al. Anatomical and chemical studies of vitrified shoots of
• ated in vito. Physiol Plant, 1985, 65: 177-- l84
• 18 Bornman C H and Vogelrnann T C. Effect of rigidity of gel medium on benzyiadenine-induced adventitiope
• tion and vittification in vitro in Picea abies. Physioi Plant. l984. 61: 505- 512
• 19 Debergh P C. Effects of agar brand and concentralion on the tissue culture medium. Physiol Plant. l9S3, 59
• 20 Etiksen E N. Root formation in pear cuttings, the influence of cytokinin at different deveIopmfntal stagre
• Plant, l974,30: l63--l67
• 2l Staden J V and Harty A R. Cytokinins ard adventitious root formatiun. in Adventitioas root formation in
• T. D. Davis, B. E. Haissig and N. E. Sankhal ). Dioscorides Press, l988. 185-- 201