苹果优良矮砧S_(18)、s_(20)微茎尖脱毒组织培养技术研究Techniques for Eliminating the Virus by Microstem Tissue Culture for Superior Apple Dwarf Rootstocks S_(18) and S_(20)
朱文勇,赵玉军,郭黄平,杜学梅,贺小红
摘要(Abstract)：
通过对武乡海棠(Malus honanensis Rehd.)优良矮砧S_(18)、S_(20)两个单系的0.2mm微茎尖脱毒组织培养技术研究,解决了微茎尖切割、接种、分化及生根移栽等一系列技术难题,获得了S_(20)、S_(18)一批脱毒试管苗及移栽壮苗.分化数分别达2.0和3.6,生根率达76.1%和90.0%,平均根长2cm以上,平均根数5条,移栽成活率达78%.
关键词(KeyWords)： 武乡海棠;微茎尖;脱毒;分化;生根;移栽
基金项目(Foundation):
作者(Author): 朱文勇,赵玉军,郭黄平,杜学梅,贺小红
DOI: 10.13925/j.cnki.gsxb.1995.04.003
参考文献(References)：
- [1]王际轩.苹果砧木组织培养繁殖研究.见:张子明编.果树砧木论文集.西安:陕西科学技术出版社,1985.128—132
- [2]山西省农科院果树研究所、S系苹果矮化砧选育研究.内部资料,1991
- [3]韩礼星,王继世,何水涛等.苹果病毒病的脱毒研究.果树科学,1991,8(2):7—12
- [4]Faust M.Physiology of Temprate Zone Fruit Trees.New york:A Wiley-Interscience Publication,1989.20—40,112—206
- [5]Mantell SH,Smith H.Plant Biotechnology.The Press Syndicate of the Universiy of Cambridge,1983.5—15
- [6]walkey DG.Prodcction of Virus-iree Plants by Tissue Culture.Blackwell Scientific Publications,1980.109—117
- [7]Quak F.Meristem culture and virus free plants.In.Appied and Fundamental Aspects of plant Cell,Tissue and Organ Culture.ed.J.Reinert ＆Y.P.S. Bajaj.New York:Springer—Verlag,598—615
- [8]Mengel K.Principles of Plant Nutrition.Int.Potash lnst,1978.22—25
- [9]裘维蕃.植物病毒学.北京:科学技术出版社,1985.5—18,67—100
- [10]Dodds John H.Tissue Culture of Trees.the AVi Publishing Company,Inc,1983.56—80