邓红红,汪卫星,李晔,向素琼,郭启高,何桥,李晓林,梁国鲁

• 1:西南大学园艺园林学院

摘要(Abstract)：

【目的】为了初步探讨宜昌橙(Citrus ichangensis Swingle)体细胞染色体联会发生的机制,【方法】以中国农业科学院国家种质重庆柑橘圃(CRICAAS)提供的宜昌橙为试验材料,利用荧光原位杂交技术(fluorescence in situ hy-bridization,FISH)研究了其体细胞有丝分裂中期染色体上45S rDNA的分布情况,并结合联会区域比较分析。【结果】结果表明,不同类型宜昌橙细胞分裂中期染色体45SrDNA的分布位点无明显差异,均存在4个杂交位点,分别位于第2对同源染色体的短臂端部或者近端部,及第9对染色体的次缢痕区域。【结论】宜昌橙体细胞染色体联会现象并非随机生物现象,而是宜昌橙固有的细胞学特征;其体细胞染色体联会以同源染色体联会为主,推测可能与异染色质集中区域或者结构形成有关。

关键词(KeyWords)： 宜昌橙;体细胞;染色体联会;荧光原位杂交;异染色质

Abstract:

Keywords:

基金项目(Foundation): 国家大学生创新性实验计划项目(101063511);; 中央高校基本科研业务费专项基金项目(XDJK2011D011);; 农业部948项目(2011-G21(2))

作者(Author): 邓红红,汪卫星,李晔,向素琼,郭启高,何桥,李晓林,梁国鲁

DOI: 10.13925/j.cnki.gsxb.2012.06.011

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