刘丽锋;古勤生;

• 1:中国农业科学院郑州果树研究所

摘要(Abstract)：

西瓜是我国重要的经济作物,其面积和产量居世界水果前列。由于西瓜资源的遗传背景相对狭窄,对于一些重要病害缺乏可利用的抗性材料,采用转基因手段有助于创制新种质。转基因技术是生物遗传改良和基因功能验证的重要方法,而组织培养和遗传转化是植物转基因成功与否的2个前提条件。西瓜遗传转化采用的方法有多种,而农杆菌介导法应用最广泛。大量的研究表明,西瓜是被公认比较难于转化的作物,目前为止转化效率低仍然是阻碍西瓜转基因的主要瓶颈。本文分析了西瓜组织培养的影响因素,如西瓜的种子贮存时间、基因型、苗龄、外植体类型、激素组合、培养条件;同时分析了农杆菌介导法影响西瓜遗传转化效率的主要因素,包括筛选标记基因、农杆菌菌株类型、预培养时间、农杆菌侵染时间和浓度、共培养时间;探讨了目前西瓜组织培养和遗传转化体系所存在的问题,提出了今后的研究方向。

关键词(KeyWords)： 西瓜;组织培养;遗传转化

Abstract:

Keywords:

基金项目(Foundation): 国家现代产业技术体系(CARS-26-13);; 中国农业科学院创新工程(CAAS-ASTIP-2016-ZFRI);; 国家自然科学基金(31572147);; 中央级科研院所基本科研业务费专项(1610192017502)

作者(Author): 刘丽锋;古勤生;

Email:

DOI: 10.13925/j.cnki.gsxb.20160362

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