王文文;宗晓娟;魏海蓉;王甲威;朱东姿;谭钺;刘庆忠;

• 1:山东省果树研究所·山东省果树生物技术育种重点实验室

摘要(Abstract)：

【目的】初步探明患有皱叶病的甜樱桃树体内是否感染病毒,为皱叶病的分子生物学鉴定及综合防治提供可靠的科学依据。【方法】以嫁接在3种不同砧木上的甜樱桃品种‘红灯’叶片为试验材料,提取植物样本总RNA,采用随机六聚体引物反转录,选取10种甜樱桃病毒作为检测对象,根据各病毒基因组序列设计特异引物,进行RT-PCR检测。【结果】10种甜樱桃待检病毒中,5种病毒检测结果呈阳性,分别为PNRSV、PDV、CVA、CGRMV、LCh V-2。各皱叶病甜樱桃样品均至少同时感染2种病毒,多病毒复合感染比例较高。【结论】甜樱桃皱叶病树体内确有病毒存在,但关于病毒是否是引起皱叶病的原因还有待进一步验证。

关键词(KeyWords)： 甜樱桃;皱叶病;砧木;病毒;检测

Abstract:

Keywords:

基金项目(Foundation): 农业部公益性行业(农业)科研专项(No.201203075);; “十二五"国家科技支撑计划”主要果树新品种选育项目;; “樱桃新品种选育”子课题(2013BAD02B03-3-2);; 山东省现代农业产业技术体系水果创新团队专项基金;; 山东省农业良种工程项目(2013)

作者(Author): 王文文;宗晓娟;魏海蓉;王甲威;朱东姿;谭钺;刘庆忠;

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