柳凤,韦继光,詹儒林,常金梅

• 1:广西大学农学院
• 2:中国热带农业科学院南亚热带作物研究所·海南省热带园艺产品采后生理与保鲜重点实验室

摘要(Abstract)：

【目的】为了解我国杧果畸形病病原菌Fusarium mangiferae的营养亲和性及营养亲和群类型,【方法】以采自四川攀枝花市和云南华坪县等不同地区和不同杧果品种的38个畸形病病原菌为材料,在含KClO3培养基上诱导筛选。【结果】共获得抗氯酸盐、不能还原利用硝酸盐营养突变体(nit)455株,通过MM、NM、HM等3种不同氮源培养基划分出nit A、nit B、nit C、nit D 4种突变类型,其中nitA出现频率最高,占总体的78.02%;nit B和nit C其次,分别占7.91%和13.63%;nitD最少,仅占0.44%。采用nit突变体互补型配对技术,将获得的突变菌株进行配对培养,测得不同的营养体亲合群(VCGs)数为5个,其中VCG1内包含30个菌株,3个菌株分布在VCG2内,VCG3和VCG4内各含有2个菌株,菌株MG33单独形成VCG5。【结论】我国杧果畸形病病原菌存在丰富的营养亲和群类型,分析发现F.mangiferae的营养亲和群种类和杧果品种、地理来源间无明显相关性。

关键词(KeyWords)： 杧果畸形病;nit突变体;抗氯酸盐突变体;营养亲和群

Abstract:

Keywords:

基金项目(Foundation): 中央级非盈利性研究机构基本科研业务费研究专项(1630062012008);; 海南省自然科学基金(808183)

作者(Author): 柳凤,韦继光,詹儒林,常金梅

DOI: 10.13925/j.cnki.gsxb.2012.06.004

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