王海波;王帅;王孝娣;史祥宾;王宝亮;郑晓翠;王志强;冀晓昊;刘凤之;

• 1:中国农业科学院果树研究所·农业部园艺作物种质资源利用重点实验室

摘要(Abstract)：

【目的】明确6-BA及氨基酸硒在激素水平上对葡萄叶片衰老的调控,为设施葡萄叶片衰老延缓技术的建立提供理论依据。【方法】在设施葡萄延迟栽培条件下,以叶片衰老速度不同的‘意大利’和‘无核白鸡心’2个葡萄品种为试材,分别进行叶面喷施6-BA和氨基酸硒处理,以清水为对照,测定不同处理和对照叶片衰老期间功能叶片的叶绿素含量和净光合速率(Pn)及内源激素含量与比值的变化。【结果】外源6-BA和氨基酸硒处理显著延缓了叶片叶绿素含量和净光合速率的下降,明显提高了玉米素核苷(ZR)和赤霉素(GA3)含量和ZR/ABA(脱落酸)、GA3/ABA、(ZR+GA3)/ABA比值,显著降低了ABA含量。生长素(IAA)具有前期保持叶片生长发育和后期促进衰老的双重作用。2个葡萄品种间比较,‘意大利’叶片衰老缓慢。【结论】6-BA和氨基酸硒通过维持较高的GA3/ABA、ZR/ABA和(GA3+ZR)/ABA比值,提高了葡萄叶片的叶绿素含量和净光合速率,延长了功能期,因此,外源喷施6-BA和氨基酸硒是延缓葡萄叶片衰老的重要技术措施。

关键词(KeyWords)： 葡萄;叶片衰老;6-BA;氨基酸硒;内源激素

Abstract:

Keywords:

基金项目(Foundation): 中国农业科学院科技创新工程(CAAS-ASTIP-2015-RIP-04);; 国家现代农业产业技术体系建设专项(nycytx-30-zp);; 948重点项目“鲜食葡萄新品种及设施化生产技术引进与创新应用”(2011-G28)

作者(Author): 王海波;王帅;王孝娣;史祥宾;王宝亮;郑晓翠;王志强;冀晓昊;刘凤之;

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参考文献(References)：

• [1]王帅,王海波,王孝娣,史祥宾,王宝亮,郑晓翠,刘凤之.施硒和6-BA对葡萄叶片衰老与活性氧代谢的影响[J].果树学报,2015,32(2):206-214.WANG Shuai,WANG Haibo,WANG Xiaodi,SHI Xiangbin,WANG Baoliang,ZHENG Xiaocui,LIU Fengzhi.Effects of selenium and 6-BA on leaf senescence and active oxygen metabolism in grape[J].Journal of Fruit Science,2015,32(2):206-214.
• [2]ZIMMERMANN P,ZENTGRAF U.The correlation between oxidative stress and leaf senescence during plant development[J].Cellular and Molecular Biology Letters,2005,10(3):515.
• [3]LARA M E B,GARCIA M C G,FATIMA T,EHNE B R,LEE T K,PROELS R,TANNER W,ROITSCH T.Extracellular invertase is an essential component of cytokinin-mediated delay of senescence[J].The Plant Cell Online,2004,16(5):1276-1287.
• [4]ZAVALETA-MANCERA H A,LOPEZ-DELGADO H,LOZATAVERA H,MORA-HERRERA M,TREVILLA-GARCIA C,VARGAS-SUAREZ M,OUGHAM H.Cytokinin promotes catalase and ascorbate peroxidase activities and preserves the chloroplast integrity during dark-senescence[J].Journal of Plant Physiology,2007,164(12):1572-1582.
• [5]CHEN J G,ZHAO H Y,ZHOU X,MAO L S,CHEN X X.Fluctuation in levels of endogenous hormones after decapitation and 6-benzyl amino purine treatment in azalea,and their relationship to apical dominance[J].Scientia Horticulturae,1997,71(1):49-58.
• [6]AGAR G,TURKER M,BATTAL P,EREZ M E.Phytohormone levels in germinating seeds of Zea mays L.exposed to selenium and aflatoxines[J].Ecotoxicology,2006,15(5):443-450.
• [7]DJANAGUIRAMAN M,DEVI D D,SHANKER A K,SHEEBA J A,BANGARUSAMY U.Selenium-an antioxidative protectant in soybean during senescence[J].Plant and Soil,2005,272(1/2):77-86.
• [8]LEHOTAI N,KOLBERT Z,PETO A,FEIGL G,ORDOG A,UMAR D,TARI I,ERDEI L.Selenite-induced hormonal and signalling mechanisms during root growth of Arabidopsis thaliana L.[J].Journal of Experimental Botany,2012,63(15):5677-5687.
• [9]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000:72-75.ZOU Qi.Experimental guidance of plant physiology[M].Beijing:China Agriculture Press,2000:72-75.
• [10]PROCHAZKOVA D,WILHELMOVA N.Leaf senescence and activities of the antioxidant enzymes[J].Biologia Plantarum,2007,51(3):401-406.
• [11]FORD D M,SHIBLES R.Photosynthesis and other traits in relation to chloroplast number during soybean leaf senescence[J].Plant Physiology,1988,86(1):108-111.
• [12]陆定志,潘裕才,马跃芳,林宗达,鲍为群,金逸民,游树鹏.杂交水稻抽穗结实期间叶片衰老的生理生化研究[J].中国农业科学,1988,21(3):21-26.LU Dingzhi,PAN Yucai,MA Yuefang,LIN Zongda,BAO Weiqun,JIN Yimin,YOU Shupeng.Physiological and biochemical studies on leaf senescence at heading and grain formation stage in hybrid rice[J].Scientia Agricultura Sinica,1988,21(3):21-26.
• [13]DOWNES B P,CROWELL D N.Cytokinin regulates the expression of a soybeanβ-expansin gene by a post-transcriptional mechanism[J].Plant Molecular Biology,1998,37(3):437-444.
• [14]GERM M,KREFT I,OSVALD J.Influence of UV-B exclusion and selenium treatment on photochemical efficiency of photosystem II,yield and respiratory potential in pumpkins(Cucurbita pepo L.)[J].Plant Physiology and Biochemistry,2005,43(5):445-448.
• [15]ANANIEVA K,MALBECK J,KAMINEK M,STADEN J V.Changes in endogenous cytokinin levels in cotyledons of Cucurbita pepo(zucchini)during natural and dark-induced senescence[J].Physiologia Plantarum,2004,122(1):133-142.
• [16]ZHU Y X,DAVIES P J.The control of apical bud growth and senescence by auxin and gibberellin in genetic lines of peas[J].Plant Physiology,1997,113(2):631-637.
• [17]JACKSON D,YAMAGUCHI J,BANOWETZ G M,HAKE S,JUAREZ M T.Leaf senescence is delayed in tobacco plants expressing the maize homeobox gene knotted1 under the control of a senescence-activated promoter[J].The Plant Cell,1999,11(6):1073-1080.
• [18]HUNG K T,KAO C H.Nitric oxide counteracts the senescence of rice leaves induced by abscisic acid[J].Journal of Plant Physiology,2003,160(8):871-879.
• [19]KIM J I,MURPHY A S,BAEK D,LEE S W,YUN D J,BRESSAN R A,NARASIMHAN M L.YUCCA6 over-expression demonstrates auxin function in delaying leaf senescence in Arabidopsis thaliana[J].Journal of Experimental Botany,2011,62(11):3981.
• [20]DAVIES P J.The plant hormones:their nature,occurrence,and functions[M].Springer Netherlands:Plant Hormones,1995:1-12.
• [21]LIM P O,LEE I C,KIM J,KIM H J,RYU J S,WOO H R,NAM H G.Auxin response factor 2(ARF2)plays a major role in regulating auxin-mediated leaf longevity[J].Journal of Experimental Botany,2010,61(5):1419-1430.
• [22]GHANEM M E,ALBACETE A,MARTINEZ-ANDUJAR C,ACOSTA M,ROMERO-ARANDA R,DODD I C,LUTTS S,PEREZ-ALFOCEA F.Hormonal changes during salinity-induced leaf senescence in tomato(Solanum lycopersicum L.)[J].Journal of Experimental Botany,2008,59(11):3039-3050.
• [23]TONUTTI P,CASSON P,RAMINA A.Ethylene biosynthesis during peach fruit development[J].Journal of the American Society for Horticultural Science,1991,116(2):274-279.
• [24]OHMIYA A,HAHI T.Promotion of ethylene biosynthesis in peach mesocarp discs by auxin[J].Plant Growth Regulation,2002,36(3):209-214.
• [25]ROSENVASSE S,MAYAK S,FRIEDMAN H.Increase in reactive oxygen species(ROS)and in senescence-associated gene transcript(SAG)levels during dark-induced senescence of Pelargonium cuttings,and the effect of gibberellic acid[J].Plant Science,2006,170(4):873-879.
• [26]SAKS Y,VAN S J.Evidence for the involvement of gibberellins in developmental phenomena associated with carnation flower senescence[J].Plant Growth Regulation,1993,12(1/2):105-110.
• [27]SHIBAOKA H,THIMANN K V.Antagonisms between kinetin and amino acids experiments on the mode of action of cytokinins[J].Plant Physiology,1970,46(2):212-220.
• [28]郑莎莎,孙传范,孙红春,刘连涛,赵金峰,李存东.不同外源激素对花铃期棉花主茎叶生理特性的影响[J].中国农业科学,2009,42(12):4383-4389.ZHENG Shasha,SUN Chuanfan,SUN Hongchun,LIU Liantao,ZHAO Jinfeng,LI Cundong.Effects of different exogenous hormones on physiological characteristics of main stem leaves at flower and boll stage in cotton[J].Scientia Agricultura Sinica,2009,42(12):4383-4389.
• [29]DODD I C.Root-to-shoot signalling:assessing the roles of‘up’in the up and down world of long-distance signalling in planta[M].Springer Netherlands:Root Physiology,2005:251-270.
• [30]赵平,林克惠,郑毅.氮钾营养对烟叶衰老过程中内源激素与叶绿素含量的影响[J].植物营养与肥料学报,2005,11(3):379-384.ZHAO Ping,LIN Kehui,ZHENG Yi.Effect of N and K nutrition on chlorophyll content and endogenous hormones in the process of tobacco senescence[J].Journal of Plant Nutrition and Fertilizer,2005,11(3):379-384.
• [31]史国安,郭香凤,张国海,包满珠.芍药花开放与衰老过程中生理指标的变化[J].西北植物学报,2008,28(3):506-511.SHI Guo’an,GUO Xiangfeng,ZHANG Guohai,BAO Manzhu.Physiological changes during florescence and flower senescence of chinese penoy[J].Acta Botanica Boreali-Occidentalia Sinica,2008,28(3):506-511.
• [32]刘敬然,刘佳杰,孟亚利,王友华,陈兵林,张国伟,周治国.外源6-BA和ABA对不同播种期棉花产量和品质及其棉铃对位叶光合产物的影响[J].作物学报,2013,39(6):1078-1088.LIU Jingran,LIU Jiajie,MENG Yali,WANG Youhua,CHEN Binglin,ZHANG Guowei,ZHOU Zhiguo.Effect of 6-BA and ABA applications on yield,quality and photosynthate contents in the subtending leaf of cotton with different planting dates[J].Acta Agronomica Sinica,2013,39(6):1078-1088.
• [33]王宁宁,刘汝,朱亮基,汤兆达.亚硒酸钠对烟草冠瘿组织生长的影响及其与内源激素的关系[J].植物生理学报,1992,18(2):160-166.WANG Ningning,LIU Ru,ZHU Liangji,TANG Zhaoda.The relationship between the effect of sodium selenite on the growth of nicotiana tabacum crown gall tissue and the level of endogenous hormones[J].Plant Physiology Journal,1992,18(2):160-166.