采用RT—PCR技术检测苹果病毒
张开春,侯义龙,胡文玉,吴禄平,林珂,张晓明
摘要(Abstract):
研究建立了危害仁果类果树的苹果褪绿叶斑病毒(ACLSV)、苹果茎沟病毒(ASGV)和苹果茎痘病毒(ASPV)的RT—PCR检测体系。其检测灵敏度高,专一性强,为仁果类果树的病毒检测提供了一种分子生物学检测方法。应用该体系对田间的苹果树携带上述三种病毒的情况进行了调查,发现单独感染率和复合率均很高,达84.2%~100%。
关键词(KeyWords): 逆转录;聚合酶链式反应;苹果;病毒;检测
基金项目(Foundation):
作者(Author): 张开春,侯义龙,胡文玉,吴禄平,林珂,张晓明
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