外源纤维素对樱桃根域土壤微生物的影响Effects of exogenous cellulose on soil microbia in cherry rooting zone
周文杰;张鹏;秦嗣军;吕德国;
摘要(Abstract):
【目的】研究外源添加纤维素对樱桃根域土壤微生物功能多样性等指标的影响,为外源添加有机物料调控樱桃根域环境、促进植株生长发育提供理论依据。【方法】以盆栽甜樱桃幼苗为试材,品种为‘美早’,砧木为东北山樱。土壤中外源添加纤维素量为4 g·kg-1干土,检测处理后7~60 d根域土壤纤维素酶活性、微生物量碳含量、有机质含量及微生物功能多样性指标的变化。【结果】外源纤维素处理前期显著提高了土壤纤维素酶活性,处理后7 d,纤维素酶活性与对照相比增加了145.3%,但随着时间的延长最后均恢复到原来的水平。外源纤维素处理后15 d,土壤微生物量碳含量达到最大,与对照相比增加了34.8%。外源纤维素处理明显改变了土壤微生物群落组成,显著提高了30 d土壤微生物的Shannon指数、Simpson指数与Mc Intosh指数及土壤微生物对碳水化合物、羧酸、酚酸类及胺类碳源的代谢能力,在7 d及15 d时土壤微生物表现出较高水平的群落代谢特征。外源纤维素处理后7~30 d,土壤有机质含量显著高于对照,60 d与对照无显著差异,土壤有机质含量在处理后7 d达到最大值,与对照相比增加了30.2%。【结论】外源纤维素处理可显著改变樱桃根域土壤微生物结构与活性,但处理效应随着时间的延长而逐渐减弱。
关键词(KeyWords): 樱桃;纤维素;微生物;土壤微生物功能多样性
基金项目(Foundation): 国家自然科学基金(30871688、30900967);; 高等学校博士学科点专项科研基金(20122103110012)
作者(Author): 周文杰;张鹏;秦嗣军;吕德国;
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