周文杰;张鹏;秦嗣军;吕德国;

• 1:沈阳农业大学园艺学院·北方果树栽培与生理生态重点实验室

摘要(Abstract)：

【目的】研究外源添加纤维素对樱桃根域土壤微生物功能多样性等指标的影响,为外源添加有机物料调控樱桃根域环境、促进植株生长发育提供理论依据。【方法】以盆栽甜樱桃幼苗为试材,品种为‘美早’,砧木为东北山樱。土壤中外源添加纤维素量为4 g·kg-1干土,检测处理后7~60 d根域土壤纤维素酶活性、微生物量碳含量、有机质含量及微生物功能多样性指标的变化。【结果】外源纤维素处理前期显著提高了土壤纤维素酶活性,处理后7 d,纤维素酶活性与对照相比增加了145.3%,但随着时间的延长最后均恢复到原来的水平。外源纤维素处理后15 d,土壤微生物量碳含量达到最大,与对照相比增加了34.8%。外源纤维素处理明显改变了土壤微生物群落组成,显著提高了30 d土壤微生物的Shannon指数、Simpson指数与Mc Intosh指数及土壤微生物对碳水化合物、羧酸、酚酸类及胺类碳源的代谢能力,在7 d及15 d时土壤微生物表现出较高水平的群落代谢特征。外源纤维素处理后7~30 d,土壤有机质含量显著高于对照,60 d与对照无显著差异,土壤有机质含量在处理后7 d达到最大值,与对照相比增加了30.2%。【结论】外源纤维素处理可显著改变樱桃根域土壤微生物结构与活性,但处理效应随着时间的延长而逐渐减弱。

关键词(KeyWords)： 樱桃;纤维素;微生物;土壤微生物功能多样性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30871688、30900967);; 高等学校博士学科点专项科研基金(20122103110012)

作者(Author): 周文杰;张鹏;秦嗣军;吕德国;

Email:

DOI:

参考文献(References)：

• [1]DINESH K M,SANDEEP K,NARENDRA K M,DHARA P,MEENU S.Nutrient availability and management in the rhizosphere by microorganisms[M].Bacteria in Agrobiology:Stress Management,2012:301-326.
• [2]RUAN H H,ZOU X M,SCATENA F N,ZIMMERMAN J K.Asynchronous fluctuation of soil microbial biomass and plant litterfall in a tropical wet forest[J].Plant and soil,2004,260(1):147-154.
• [3]MA L,HUANG W W,GUO C Y.Soil microbial properties and plant growth responses to carbon and water addition in a temperate steppe:the importance of nutrient availability[J].Plos One,2012,7(4):1-12.
• [4]ZHANG W J,ZHU W,HU S.Soil resource availability impacts microbial response to organic carbo n and inorganic nitrogen inputs[J].Journal of Environmental Sciences,2005,17(5):705-710.
• [5]DRENOVSKY R E,VO D,GRAHAM K J,SCOW K M.Soil water content and organic carbon availability are major determinants of soil microbial community composition[J].Microbial Ecology,2004,48(3):424-430.
• [6]WANG Nian,ZHANG Kai-chun,WANG Jing,ZHANG Xiaoming,YAN Guo-hua.Preliminary study on polymorphism of ACO gene in six varieties of sweet cherry(Prunus avium)[J].Journal of Fruit Science,2012,29(3):359-365.王廿,张开春,王晶,张晓明,闫国华.6个甜樱桃品种ACO基因多态性的检测[J].果树学报,2012,29(3):359-365.
• [7]LIU Bao-you,ZHANG Wei,LUAN Bing-hui,WANG Ying-zi.Identification of pathogen and epidemic dynamics of brown spot of sweet cherry[J].Journal of Fruit Science,2012,29(4):634-637.刘保友,张伟,栾炳辉,王英姿.大樱桃褐斑病病原菌鉴定与田间流行动态研究[J].果树学报,2012,29(4):634-637.
• [8]LIU Zhi-heng,BAI Hai-tao,YANG Hong,TANG Shuang-shuang,WEI Mei-na,HUANG Xin-yang,LI Yu-tao.Biological characteristics of Monilia fructigena as pathogen of brown rot in sweet cherry[J].Journal of Fruit Science,2012,29(3):423-427.刘志恒,白海涛,杨红,唐爽爽,魏美娜,黄欣阳,李俞涛.大樱桃褐腐病菌生物学特性研究[J].果树学报,2012,29(3):423-427.
• [9]LDe-guo,QIN Si-jun,LIU Guo-cheng,DU Guo-dong.Cherr root biology[M].Beijing:Science Press,2011.吕德国,秦嗣军,刘国成,杜国栋.樱桃根系生物学[M].北京:科学出版社,2011.
• [10]VANCE E D,BROOKES P C,JENKINSON D S.An extraction method for measuring microbial biomass C[J].Soil Biology&Biochemistry,1987,19:703-707.
• [11]GUAN Song-yin.Study way of soil enzymes[M].Beijing:Agriculture Press,1986.关松荫.土壤酶及其研究法[M].北京:农业出版社,1986.
• [12]BORRERO C,ORDOVA’S J,TRILLAS M I,AVILS M.Tomato Fusarium wilt suppressiveness:The relationship between the organic plant growth media and their microbial communities as characterised by Biolog[J].Soil Biology&Biochemistry,2006,38:1631-1637.
• [13]ISLAM M R,CHAUHAN P S,KIM Y,KIM M,SA T.Community level functional diversity and enzyme activities in paddy soils under different long-term fertilizer management practices[J].Biol Fertil Soils,2011,47:599-604.
• [14]FONTAINE S,BARDOUX G,BENEST D,VERDIER B,MARIOTTI A,ABBADIC L.Mechanisms of the priming effect in a Savannah soil amended with cellulose[J].Soil Sci Soc Am J,2004,68:125-131.
• [15]BLAGODATSKAYA E V,KUZYAKOV Y.Mechanisms of real and apparent priming effects and their dependence on soil microbial biomass and community structure:critical review[J].Biol Fertil Soils,2008,45:115-131.
• [16]KUZYAKOV Y,BOL R.Sources and mechanisms of priming effect induced in two grassland soils amended with slurry and sugar[J].Soil Biol Biochem,2006,38:747-758.
• [17]BU Hong-zhen,WANG Li-hong,YOU Jin-cheng,XIAO Xiaoping,YANG Guang-li,HU Yue-gao,ZENG Zhao-hai.Impact of Long-Term fertilization on the microbial biomass carbon and soil microbial communities in paddy red soil[J].Scientia Agricultura Sinica,2010,43(16):3340-3347.卜洪震,王丽宏,尤金成,肖小平,杨光立,胡跃高,曾昭海.长期施肥管理对红壤稻田土壤微生物量碳和微生物多样性的影响[J].中国农业科学,2010,43(16):3340-3347.
• [18]BROOKES P C.The use of microbial parameters in monitoring soil pollution by heavy metals[J].Biology and Fertility of Soil,1995,19:269-279.
• [19]SPARLING G P,PANKHURST C,DOUBE B M,GUPTA V V S R.“Soil microbial biomass,activity and nutrient cycling as indicators of soil health.”Biological indicators of soil health[M].Cab International,1997.
• [20]BLAGODATSKAYA E V,BLAGODATSKY S A,ANDERSON T H,KUZYAKOV Y.Priming effects in Chernozem induced by glucose and N in relation to microbial growth strategies[J].Applied soil ecology,2007,37:95-105.
• [21]PANIKOV N S.In:Glasgow,et al.(Eds.),Microbial Growth Kinetics[M].Chapman and Hall,London,1995.
• [22]ZABINSKI C A,GANNON J E.Effects of recreational impacts on soil microbial communities[J].Environmental Management,1997,21(2):233-238.
• [23]GARLAND J L.Analysis and interpretation of community-level physiological profiles in microbial ecology[J].FEMS Microbiology Ecology,1997,24:289-300.
• [24]STADDON W J,DUCHESNE L C,TREVORS J T.Microbial diversity and community structure of postdisturbance forest soils as determined by sole-carbon-source utilization patterns[J].Microbiology Ecology,1997,34:125-130.
• [25]KONG Wei-dong,LIU Ke-xing,LIAO Zong-wen,ZHU Yongguan,WANG Bi-ling.Effects of organic matters on metabolic functional diversity of soil microbial community under pot incubation conditions[J].Acta Ecologica Sinica,2005,9(25):2291-2296.孔维栋,刘可星,廖宗文,朱永官,王碧玲.不同腐熟程度有机物料对土壤微生物群落功能多样性的影响[J].生态学报,2005,9(25):2291-2296.
• [26]LIU Ming,LI Zhong-pei,LU Lei,CHE Yu-ping.Changes in soil respiration and microbial functional diversity of paddy soil under different fertilizer amendments[J].Scientia Agricultura Sinica,2009,42(3):1108-1115.刘明,李忠佩,路磊,车玉萍.添加不同养分培养下水稻土微生物呼吸和群落功能多样性变化[J].中国农业科学,2009,42(3):1108-1115.
• [27]LI Zhong-pei,WU Xiao-chen,CHEN Bi-yun.Changes in transformation of soil organic carbon and functional diversity of soil microbial community under different land use patterns[J].Scientia Agricultura Sinica,2007,40(8):1712-1721.李忠佩,吴晓晨,陈碧云.不同利用方式下土壤有机碳转化及微生物群落功能多样性变化[J].中国农业科学,2007,40(8):1712-1721.
• [28]LI Fang-liang,LI Zhong-pei,LIU Ming,JIANG Chun-yu,CHE Yu-ping.Effects of glucose and ammonium sulfate addition on paddy soil microbial biomass and functional diversity[J].Scientia Agricultura Sinica,2012,45(11):2199-2208.栗方亮,李忠佩,刘明,江春玉,车玉萍.添加葡萄糖及硫酸铵对水稻土微生物生物量和功能多样性的影响[J].中国农业科学,2012,45(11):2199-2208.
• [29]HU J L,LIN X G,WANG J H,DAI J,CHEN R R,ZHANG J B,WONG M H.Microbial functional diversity,metabolic quotient,and invertase activity of a sandy loam soil as affected by long-term application of organic amendment and mineral fertilizer[J].J Soils Sediments,2011,11:271-280.
• [30]GOMEZ E,FERRERAS L,TORESANI S.Soil bacterial functional diversity as influenced by organic amendment application[J].Bioresour Technol,2006,97:1484-1489.