徐小彪;廖娇;黄春辉;辜青青;曲雪艳;刘善军;陈金印;

• 1:江西农业大学农学院

摘要(Abstract)：

利用开发的11对EST-SSR引物分析了33份猕猴桃种质资源的遗传多样性及其遗传关系。结果表明,11对EST-SSR引物在所有供试材料中均可扩增出清晰条带,其中有8对引物呈现多态性,多态性扩增率为72.7%,对33份种质材料的区分率达100%。8对多态性引物共检测到61个等位基因,每对引物可检测到的等位基因数为2-17,平均为7.6个。利用NTSYS-pc软件,以不加权成对算术平均法(UPGMA)对扩增结果进行聚类,谱系图显示,33份种质材料之间的相似系数在0.60～0.97,表明猕猴桃种质之间遗传关系相对来说不是很远。在相似系数0.73的水平上可将供试猕猴桃材料分为7个类群,其结果与传统的形态分类大体一致。从分子角度揭示了猕猴桃种质资源的遗传多态性及其遗传关系。可为猕猴桃种质改良提供理论依据。EST-SSR是非常有效和可靠的分子标记,可为猕猴桃分子育种及遗传连锁图构建奠定基础。

关键词(KeyWords)： 猕猴桃;EST-SSR;种质资源;遗传分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(No.30860167);; 科技部国际科技合作项目(2011DFA31050)

作者(Author): 徐小彪;廖娇;黄春辉;辜青青;曲雪艳;刘善军;陈金印;

Email:

DOI: 10.13925/j.cnki.gsxb.2012.02.012

参考文献(References)：

• [1]WARRINGTON I J,WESTON G C.Kiwifruits:Science an d Man-agement[M].Ray Richards Publisher,1990:183-204.
• [2]LI Qi,LI Zuo-zhou,HUANG Hong-wen.Preliminary study on SSRanalysis in natural populations of Actinidia[J].Journal of WuhanBotanical Research,2004,22(2):175-188.栗琪,李作洲,黄宏文.猕猴桃野生居群的SSR分析初报[J].武汉植物学研究,2004,22(2):175-188.
• [3]WEISING K,FUNG R W M,KEELING D J,ATKINSON R G,GARDNER R C.Characterisation of microsatellites from Actinidiachinensis[J].Molecular Breeding,1996,2(2):117-131.
• [4]HUANG W G,CIPRIANI G,MORGANTE M,TESTOLIN R.Mi-crosatellite DNA in Actinidia chinensis:Isolation,characterizationand homology in related species[J].Theoretical and Applied Genet-ics,1998,97(8):1269-1278.
• [5]JIA Bing,ZHU Li-wu,YU Xing,LU Li-juan,WANG Yan-fang.RAPD analysis on germplasm resources in genera Actinidia[J].Jour-nal of Anhui Agricultural University,2005,32(3):381-384.贾兵,朱立武,余兴,陆丽娟,王艳芳.猕猴桃种质资源RAPD分析[J].安徽农业大学学报,2005,32(3):381-384.
• [6]ZOU You,DING Jian,SHEN Ying,ZHAO Jian,YANG Zhi-rong,WU Cheng.Analysis of random amplified polymorphic DNA in 11Actinidia varieties[J].Chinese Journal of Applied&EnvironmentalBiology,2007,13(2):172-175.邹游,丁建,申瑛,赵建,杨志荣,吴成.11个猕猴桃品种间的遗传多样性分析[J].应用与环境生物学报,2007,13(2):172-175.
• [7]NING Yun-ye,XIONG Qing-e,ZENG Wei-guang,ZENG Guang-rong.Studies on red flesh sport from Red Sun Kiwifruit using RAPDmarker[J].Acta Horticulturae Sinica,2003,30(5):511-513.宁允叶,熊庆娥,曾伟光,曾光荣.红阳猕猴桃全红芽变系的RAPD分析[J].园艺学报,2003,30(5):511-513.
• [8]DONG Xiao-li,TANG Hao-ru,DING Jian,LI Ming-zhang,ZHENGXiao-qin,YANG Zu-jun.Analysis of the genetic diversity betweenActinidia deliciosa var.coloris and A.deliciosa var.deliciosa[J].Journal of Fruit Science,2006,23(5):676-680.董晓莉,汤浩茹,丁建,李明章,郑晓琴,杨足君.彩色猕猴桃与美昧猕猴桃的遗传差异分析[J].果树学报,2006,23(5):676-680.
• [9]CHEN Hua,YI Gan-iun,XU Xiao-biao.Identification of the ki-wifruit germplasms in Jiangxi province by AFLP and its classificationsignificance[J].Chinese Journal of Biochemistry and Molecular Biol-ogy,2007,23(2):122-129.
• [10]VARSHNEY R K,GRNAER A,SORRELLS M E.Genetic mi-crosatellite markers in plants:Features and applications[J].Trends inBiotechnology,2005,23:48-55.
• [11]YAO Li-hua,TENG Yuan-wen.A brief introduction on EST-SSRmarker and its application in the research of fruit trees[J].Journal ofFruit Science,2008,25(2):219-224.姚利华,滕元文.EST-SSR标记及其在果树研究中的应用[J].果树学报,2008,25(2):219-224.
• [12]MURRAY M G,THOMSPON W F.Rapid isolation of high molecularweight plant DNA[J].Nucleic Acids Research,1980,8:4321-4326.
• [13]XU Xiao-biao,CHEN Hua,ZHANG Qiu-ming.Effects of differentstoring condition on total DNA extraction of kiwifruit[J].Acta Agri-culturae Universitatis Jiangxiensis,2004,26(3):321-323.徐小彪,陈华,张秋明.样品不同保存方法对猕猴桃总DNA提取效果的影响[J].江西农业大学学报,2004,26(3):321-323.
• [14]SCOTT K D,EGGLER P,SEATON G,ROSSETTO M,ABLETT EM,LEE L S,HENRY R J.Analysis of SSRs derived from grape ESTs[J].Theoretical and Applied Genetics,2000,1:723-726.
• [15]YAMAMOTO T,KIMURA T,SAWAMURA Y,KOTOBUKY K,BAN Y,HAYASHI T,MATSUTA N.SSRs isolated from apple canidentify polymorphism and genetic diversity in pear[J].Theoreticaland Applied Genetics,2001,102:865-870.
• [16]CHEN C X,ZHOU P,CHOI Y A,HUANG S,GMITTER F G.Miningand characterizing microsatellites from citrus ESTs[J].Theoreticaland Applied Genetics,2006,112:1248-1257.
• [17]XIE H,SUI Y,CHANG F Q,XU Y,MA R C.SSR allelic variationin almond(Prunus dulcis Mill.)[J].Theoretical and Applied Genet-ics,2006,112:366-372.
• [18]WANG J Y,CHEN Y Y,LIU W L,WU Y T.Development and ap-plication of EST derived SSR markers for bananas(Musa nana Lour.)[J].Hereditas,2008,30(7):933-940.
• [19]XU Xiao-biao,JIANG Chun-ya,LIAO Jiao,GU Qing-qing,LIUShan-jun,CHEN Jin-yin.Screening of EST-SSR marker linked todwarf character in Actinidia chinensis Planch[J].Acta HorticulturaeSinica,2010,37(4):553-558.徐小彪,姜春芽,廖娇,辜青青,刘善军,陈金印.中华猕猴桃矮型性状EST-SSR连锁标记的筛选[J].园艺学报,2010,37(4):553-558.
• [20]SILFVERBERG D E,MATASCI C L,VANDE W E,VAV KAAUWENM P W,WALSER M,KODDE L P,SOGLIO V,GIANFRANCESHIL,DUREL C E,COSTA,F.Microsatellite markers spanning theapple(Malus×domestica Borkh.)genome[J].Tree Genetics andGenomes,2006,2:202-224.
• [21]SARGENT D J,CLARKE J,SIMPSON D W,TOBUTT K R,ARS P,MONFORT A,VILANOVA S,DENOYES-ROTHAN B,ROUSSEAU M,FOLTA K M,BASSIL N V,BATTEY N H.An en-hanced microsatellite map of diploid Fragaria[J].Theoretical andApplied Genetics,2006,112:1349-1359.
• [22]CHEN C X,BOWMAN K D,CHOI Y A,DANG P M,RAO M N,HUANG S,SONEJI J R,MCCOLLUM T G,GMITTER F G.EST-SSR genetic maps for Citrus sinensis and Poncirus trifoliate[J].TreeGenetics&Genomes,2008,4:1-10.
• [23]FRASER L G,HAVREY C F,CROWHURST R N,SILVA H N.EST-derived microsatellites from Actinidia species and their poten-tial for mapping[J].Theoretical and Applied Genetics,2004,108(6):1010-1016.
• [24]HUANG Hong-wen,GONG Jun-jie,WANG Sheng-mei,HE Zi-can,ZHANG Zhong-hui,LI Jian-qiang.Genetic diversity in thegenus Actinidia[J].Biodiversity Science,2000,8:1-12.黄宏文,龚俊杰,王圣梅,何子灿,张忠慧,李建强.猕猴桃属(Actinidia)植物的遗传多样性[J].生物多样性,2000,8:1-12.
• [25]HUANG H,FERGUSON A R.Genetic resources of kiwifruit:domes-tication and breeding[J].Horticultural Reviews,2007.
• [26]LIANG Chou-fen.On the distribution of Actinidias[J].Guihaia,1983,3(4):229-248.梁畴芬.论猕猴桃属植物的分布[J].广西植物,1983,3(4):229-248.