徐小彪;廖娇;黄春辉;辜青青;曲雪艳;刘善军;陈金印;

• 1:江西农业大学农学院

摘要(Abstract)：

利用开发的11对EST-SSR引物分析了33份猕猴桃种质资源的遗传多样性及其遗传关系。结果表明,11对EST-SSR引物在所有供试材料中均可扩增出清晰条带,其中有8对引物呈现多态性,多态性扩增率为72.7%,对33份种质材料的区分率达100%。8对多态性引物共检测到61个等位基因,每对引物可检测到的等位基因数为2-17,平均为7.6个。利用NTSYS-pc软件,以不加权成对算术平均法(UPGMA)对扩增结果进行聚类,谱系图显示,33份种质材料之间的相似系数在0.60～0.97,表明猕猴桃种质之间遗传关系相对来说不是很远。在相似系数0.73的水平上可将供试猕猴桃材料分为7个类群,其结果与传统的形态分类大体一致。从分子角度揭示了猕猴桃种质资源的遗传多态性及其遗传关系。可为猕猴桃种质改良提供理论依据。EST-SSR是非常有效和可靠的分子标记,可为猕猴桃分子育种及遗传连锁图构建奠定基础。

关键词(KeyWords)： 猕猴桃;EST-SSR;种质资源;遗传分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(No.30860167);; 科技部国际科技合作项目(2011DFA31050)

作者(Author): 徐小彪;廖娇;黄春辉;辜青青;曲雪艳;刘善军;陈金印;

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DOI:

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