葡萄斑点病毒的血清学检测技术Serological testing technique of the grapevine fleck virus
刘永清,洪霓,王国平
摘要(Abstract)：
应用A蛋白酶联免疫吸附法(ProteinASandwichEnzymeLinkImmunoSorbantAssay,PAS-ELISA)、三抗体夹心酶联免疫吸附法(TripleAntibodySandwich,TAS-ELISA)、酶联板直接捕获抗原的酶联免疫吸附法(PlateTrappedAntigen-Indirect-ELISA,PTA-I-ELISA)、直接组织印迹法(DirectTissueBlotImmunoassay,DTBIA)和圆点免疫印迹分析法(Dot-ImmunoBindingAssay,DIBA)等5种方法检测葡萄斑点病毒(GrapevineFleckVirus,GFkV)感染的葡萄枝条、叶柄和叶片。结果表明:TAS-ELISA法灵敏度最高,其粗汁液的最大稀释度为1:5120,DTBIA和DIBA法均为1:1280,PAS-ELISA法为1:640,PTA-I-ELISA法检测的灵敏度最低,为1:320。针对不同季节和葡萄不同的取样部位进行的血清学检测结果表明,以秋末冬初和葡萄中下部枝条为GFkV最佳的检测时机和器官。另外,用健康汁液吸附抗血清,可以明显降低DTBIA和DIBA的非特异性反应,便于得到直观、准确可靠的检测结果。
关键词(KeyWords)： 葡萄斑点病毒;血清学检测
基金项目(Foundation):
作者(Author): 刘永清,洪霓,王国平
Email:
DOI: 10.13925/j.cnki.gsxb.2005.03.025
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