赵婷;杨建宁;吴玉霞;张瑞;刘兵;张夏燚;高立杨;王延秀;

• 1:甘肃农业大学园艺学院
• 2:天水市麦积区果品产业局

摘要(Abstract)：

【目的】研究外源喷施硫化氢(H_2S)对盐碱胁迫下垂丝海棠幼苗光合及生理特性的影响。【方法】采用盆栽试验法,对8叶龄垂丝海棠幼苗共设6个处理,即清水浇灌(CK_1)、盐碱胁迫处理(CK_2)、盐碱胁迫+0.1 mmol·L~(-1)NaHS喷施处理(T_1)、盐碱胁迫+0.2 mmol·L~(-1)NaHS喷施处理(T_2)、盐碱胁迫+0.3mmol·L~(-1)NaHS喷施处理(T_3)、盐碱胁迫+0.4mmol·L~(-1)NaHS喷施处理(T_4),测定各处理的叶绿素含量、光合特性、荧光参数、抗氧化酶活性、相对电导率及渗透调节物质含量,并进行相关与主成分分析。【结果】随盐碱胁迫时间的延长,叶绿素a(Chl a)、叶绿素b(Chl b)、叶绿素a+b(Chl a+b)、净光合速率(P_n)、气孔导度(G_s)、蒸腾速率(T_r)、初始荧光(F_0)、光化学淬灭系数(q P)均呈下降趋势;胞间CO_2浓度(C_i)、调节性能量耗散(Y(NPQ))、非调节性能量耗散(Y(NO))、相对电导率(REC)均呈上升趋势;脯氨酸(Pro)、超氧化物歧化酶(SOD)、过氧化物酶(POD)均呈先升后降的趋势;相比CK_2,外源H_2S处理下各指标的上升和下降均有所减小,并具有浓度效应,呈现出随浓度升高先上升后下降的趋势;胁迫30 d时,各处理组的REC相比对照分别下降了22.16%、37.92%、20.92%、13.54%;Pro分别降低了17.99%、24.48%、16.58%、8.39%,表明H_2S处理具有浓度效应。T_2处理下Chl a、Chl b、Chl a+b、P_n、G_s、T_r、F_0及q P下降最少,且显著高于CK_2,低于CK_1;Y(NPQ)、Y(NO)、REC上升幅度最大;Pro、SOD、POD变化最明显。相关性分析结果表明,P_n与G_s、T_r、F_0、q P呈正相关,P_n与REC、C_i、Y(NO)、Y(NPQ)呈负相关。对上述指标标准化进行主成分分析,提取特征值>1的2个主成分,其特征值分别为14.783和2.781,方差贡献率分别为82.128%和15.452%,累计方差贡献率达到97.58%。依据主成分得分排序,外源H_2S对垂丝海棠盐碱胁迫缓解能力由高到低为:CK_1>T_2>T_1>T_3>T_4>CK_2。【结论】0.2 mmol·L~(-1)的外源H_2S可更好改善盐碱胁迫下垂丝海棠的光合能力、提高抗氧化酶活性、增加生物膜的稳定性,从而起到缓解盐碱胁迫的作用。

关键词(KeyWords)： 苹果砧木;垂丝海棠;H_2S;盐碱胁迫;光合特性;叶绿素荧光;主成分分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31960581)

作者(Author): 赵婷;杨建宁;吴玉霞;张瑞;刘兵;张夏燚;高立杨;王延秀;

Email:

DOI: 10.13925/j.cnki.gsxb.20200066

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