枇杷属植物基因组DNA提取方法的改进及其应用An improved procedure for nuclear DNA isolation from Eriobotrya plants and its application
刘月学,杨向晖,林顺权,胡桂兵,刘成明
摘要(Abstract)：
以枇杷属的普通枇杷和多种野生枇杷的叶片为材料,对枇杷的DNA提取进行了研究。针对枇杷富含多酚类 等次生物质的特点对枇杷DNA的提取步骤进行了改良处理:采用CTAB-蛋白酶K法加重复抽提,去除枇杷材料中 的相关次生物质,获得了高质量的DNA。所提取的DNA完全能满足PCR、RAPD、AFLP等一些分子生物学实验要 求。
关键词(KeyWords)： 枇杷属;DNA提取
基金项目(Foundation): 教育部骨干教师资助计划 广东省农业攻关计划项目(2004B20901001)。
作者(Author): 刘月学,杨向晖,林顺权,胡桂兵,刘成明
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参考文献(References)：
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