香蕉不同器官中microRNA差异表达分析Differential expression analysis of microRNA in banana different organs
王静毅;武耀廷;刘菊华;贾彩红;苗红霞;张建斌;王卓;金志强;徐碧玉;
摘要(Abstract)：
【目的】研究mi RNA在香蕉不同器官中的表达,初步探索mi RNA在香蕉生长发育过程中的可能作用。【方法】利用茎环引物的半定量和实时荧光定量PCR策略,检测香蕉中的保守mi RNA,并进一步研究mi RNA在香蕉根系、叶片、雄花和果实组织中的表达差异。【结果】mi RNA156d和mi RNA162a具有相似的表达模式,其最高表达量均出现在雄花组织中。mi R156d在雄花中的表达量(2.25)明显高于根系(0.17)、果实(0.34)和叶片(1.00);mi R162a在雄花中的表达量最高,为4.36,根系组织次之,为1.72,而在叶(1.00)和果实(0.99)中的表达几乎相同。mi R164e在根系中的表达量最高,为2.37,雄花次之,为1.93,而在叶(1.00)和果实(0.98)中表达几乎相同。mi R169h在4种组织中的表达量差异不明显。【结论】以香蕉各组织总RNA为模板,通过以SYBR Green为染料的Stem-loop q RT-PCR方法,成功地在香蕉根系、叶片、雄花和果实4种器官中均检测到了保守mi RNA的表达,表明本研究所建立的体系是定性或定量检测香蕉植株中保守mi RNA的准确而有效的方法。同时,在香蕉的不同组织中发现了具有显著表达差异的mi RNA,符合mi RNA在不同组织的表达活性具有多样性的规律。
关键词(KeyWords)： 香蕉;MicroRNA;实时荧光定量PCR;表达
基金项目(Foundation): 国家自然科学基金(31501043,31401843);; “十二五农村领域国家科技计划”(2011AA10020605);; 海南省自然科学基金(20153116);; 国家现代农业产业技术体系项目“国家香蕉产业技术体系”(CARS-32)
作者(Author): 王静毅;武耀廷;刘菊华;贾彩红;苗红霞;张建斌;王卓;金志强;徐碧玉;
Email:
DOI: 10.13925/j.cnki.gsxb.20150509
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