生物技术在西瓜遗传育种研究中的应用Advances of Biotechnology Utilization in Genetics and Breeding of Watermelon (Citrullus lanatus)
柳李旺,陈崇顺,陈锋,赵丽萍
摘要(Abstract)：
离体培养、基因工程与分子标记分析等生物技术应用于西瓜性状遗传改良研究取得重要进展。目前已建立了成熟的西瓜高效离体培养再生体系,主要应用于优良种苗快繁与重要性状遗传转化研究;染色体组水平的倍性操作已成功用于西瓜育种实践;在西瓜中已鉴定克隆多个重要性状基因;采用农杆菌介导或直接转化法,已将一些目的基因导入西瓜中,进行抗病、耐盐等重要性状改良;RAPD、RFLP、SSR、ISSR、AFLP等多种分子标记开始广泛应用于西瓜种质资源分析与系统演化、品种鉴定与倍性分析、遗传图谱构建、性状基因连锁标记与标记辅助育种等方面。
关键词(KeyWords)： 西瓜;离体培养;基因工程;遗传标记
基金项目(Foundation): 国家自然科学基金资助(39670515)
作者(Author): 柳李旺,陈崇顺,陈锋,赵丽萍
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