查倩;奚晓军;蒋爱丽;田益华;王世平;

• 1:上海农业科学院林木果树研究所
• 2:上海交通大学农学与生物技术学院

摘要(Abstract)：

【目的】通过ge Norm软件筛选实时荧光定量PCR稳定内参基因,基于多内参基因评价体系进行目的基因相对表达量的计算。【方法】获得不同葡萄品种叶片和果皮中6个候选内参基因的循环阈值(Ct),利用ge Norm软件计算内参基因平均表达稳定性数值M和基因配对差异值V,从而判断内参基因最适组合。【结果】发现候选内参基因表达稳定性由高到低的排列顺序为Vv EF1r=Vv EF1-α>Vv GAPDH>Vv ACTIN>Vv ACT1>Vv UBQ,不同组织分别分析均发现Vv EF1-α和Vv EF1r的稳定性最高,且基因配对差异值V2/3为0.104,所以内参基因的最适组合为Vv EF1-α和Vv EF1r。利用ge Norm软件计算得到的多内参基因评价体系的标准化因子可应用于目的基因的相对表达量分析。【结论】ge Norm软件筛选实时荧光定量PCR稳定内参基因的方法可以应用到多条件和多组织的不同植物样本中,并用于目的基因表达谱的研究,具有重要的广泛应用价值。

关键词(KeyWords)： 葡萄;ge Norm;内参基因;标准化因子;实时荧光定量PCR

Abstract:

Keywords:

基金项目(Foundation): 国家葡萄产业技术体系项目(CARS-30-9);; 上海市农委科技兴农推广项目[沪农科推字(2012)第1-2号]

作者(Author): 查倩;奚晓军;蒋爱丽;田益华;王世平;

Email:

DOI: 10.13925/j.cnki.gsxb.20150327

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