与桃果实非酸/酸性状连锁AFLP特异片段的克隆及序列分析Cloning and Sequence Analysis of AFLP Specific Fragment Related to Fruit Non-acid/acid Trait of Peach
吴俊,束怀瑞,张学宁,张开春,姜立杰
摘要(Abstract):
利用AFLP技术从京玉、美味正反交F1代69株群体中筛选到3个特异扩增片段,与桃果实非酸/酸性状紧密连锁。回收特异片段并将其克隆、测序,结果表明特异片段A、B、C均来自于EcoRI与MseI两内切酶酶切片段,大小依次为140、199、408bp,利用序列分析软件推测片段B、C中可能含有与糖水平调控作用相关的因子。上述结果对AFLP标记向SCAR标记的转换具有重要意义。
关键词(KeyWords): 桃;非酸/酸;AFLP;克隆;序列
基金项目(Foundation): 北京科技合同项目(954830300);; 北京市新星项目(953812200)。
作者(Author): 吴俊,束怀瑞,张学宁,张开春,姜立杰
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