姚全胜;雷新涛;黄忠兴;朱敏;王松标;黄丽芳;马小卫;詹儒林;武红霞;

• 1:中国热带农业科学院南亚热带作物研究所
• 2:中国热带农业科学院
• 3:广州甘蔗糖业研究所海南甘蔗育种场
• 4:中国热带农业科学院热带作物品种资源研究所

摘要(Abstract)：

以对炭疽病高抗的金煌品种为母本,高感的爱文品种为父本构建杧果炭疽病抗性杂交群体,共获得杂交果实219个,培育杂交实生种苗184株。按花朵统计结实率为2.18%。通过对184株杂交种苗进行SSR分子标记鉴定,结果表明,16对引物中15对能够有效扩增产物;12对具有丰富的多态性;8对在父母本之间具有多态性,184株杂交种苗可以确认是真实杂种,应用SSR分子标记技术可以对杧果杂种的真实性进行快速验证。

关键词(KeyWords)： 杧果;炭疽病;杂交群体构建;SSR;杂种鉴定

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30760147);; 广东省自然科学基金(5011818);; 广东省农业攻关计划项目(2005B20901026);; 农业科技成果转化资金项目(2008GB48B06);; 林业科技支撑计划项目(2006BAD01A1705);; 公益性行业科研专项经费(3-44)

作者(Author): 姚全胜;雷新涛;黄忠兴;朱敏;王松标;黄丽芳;马小卫;詹儒林;武红霞;

Email:

参考文献(References)：

• [1]WANG Wan-dong,LIU Guang-hua,NI Zhang-guang,LUO Xin-ping,YU Yan-chun.Pathogenesis and comprehensive treatment ofmango anthracnose[J].Guangdong Agricultural Sciences,2008(6):67-69.王万东,刘光华,尼章光,罗心平,俞艳春.杧果炭疽病的发生规律及综合防治[J].广东农业科学,2008(6):67-69.
• [2]LEI Xin-tao,ZHAO Yan-long,YAO Quan-sheng,HE Yan-biao,SUN Guang-ming,MA Wei-hong,WANG Song-biao,WANG Yi-cheng,LUO Wen-yang,ZHAN Ru-lin.Identification and analysis ofthe resistance of different mango cultivars to anthracnose(col-letotrichum gloeosporioides)[J].Journal of Fruit Science,2006,23(6):838-842.雷新涛,赵艳龙,姚全胜,何衍标,孙光明,马蔚红,王松标,王一承,罗文杨,詹儒林.杧果抗炭疽病种质资源的鉴定与分析[J].果树学报,2006,23(6):838-842.
• [3]YAO Quan-sheng,LEI Xin-tao,HUANG Zhong-xing,HUANG Li-fang,SU Jun-bo.Artificial pollination of mango[J].Chinese Journalof Tropical Agriculture,2009,29(1):17-19.姚全胜,雷新涛,黄忠兴,黄丽芳,苏俊波.杧果人工杂交授粉试验初报[J].热带农业科学,2009,29(1):17-19.
• [4]DUVAL M F,BUNEL J,SITBON C,RISTERUCCI A M.Develop-ment of microsatellite markers for mango(Mangifera indica L.)[J].Molecular Ecology Notes,2005,5(4):824-826.
• [5]EIADTHONG W,YONEMORI K,SUGIURA A,UTSUNOMIYAN,SUBHADRABANDHU S.Identification of mango cultivars ofThailand and evaluation of their genetic variation using the amplifiedfragments by simple sequence repeat-(SSR-)anchored primers[J].Scientia Horticulturae,1999,82(1-2):57-66.
• [6]SCHNELL R J,BROWN J S,OLANO C T,MEEROW A W,CAMPBELL R J,KUHN D N.Mango genetic diversity analysis andpedigree inferences for Florida cultivars using microsatellite markers[J].Journal of the American Society for Horticultural Science,2006,131(2):214-224.
• [7]VIRUEL M A,ESCRIBANO P,BARBIERI M,FERRI M,HOR-MAZA J I.Fingerprinting,embryo type and geographic differentia-tion in mango(Mangifera indica L.,Anacardiaceae)with mi-crosatellites[J].Molecular Breeding,2005,15(4):383-393.
• [8]WANG Jia-bao,WANG Ling-xia,LIU Zhi-yuan,YI Xiao-ping,DU Zhong-jun,XU Bi-yu.Comparison of genomic DNA extractionmMethods and optimization of ISSR-PCR reaction systems for mango(Mangifera indica L.)[J].Biotechnology,2005,15(5):37-41.王家保,王令霞,刘志媛,易小平,杜中军,徐碧玉.杧果DNA提取方法比较及ISSR反应体系的优化[J].生物技术,2005,15(5):37-41.
• [9]LEI Xin-tao,WANG Jia-bao,XU Xue-rong,LIN Shun-quan.Studies on gentic polymorphism of main mango cultivars via AFLPmarkers[J].Acta Horticulturae Sinica,2006,33(4):725-730.雷新涛,王家保,徐雪荣,林顺权.杧果主要品种遗传多态性的AFLP标记研究[J].园艺学报,2006,33(4):725-730.
• [10]TIAN Lei,GUAN Rong-xia,LIU Zhang-xiong,CHANG Ru-zhen,QIU Li-juan.Verity identification of soybean hybrids using SSRmarkers[J].Journal of Plant Genetic Resources,2008,9(4):443-447.田蕾,关荣霞,刘章雄,常汝镇,邱丽娟.用SSR标记鉴定大豆杂交组合F1的方法研究[J].植物遗传资源学报,2008,9(4):443-447.
• [11]LI Xin-lei,CHEN Fa-di,CUI Na-xin.Identification of interspecifichybrids in Dendranthema[J].Journal of Nanjing Agricultural Univer-sity,2005,28(1):24-28.李辛雷,陈发棣,崔娜欣.菊属种间杂种的鉴定[J].南京农业大学学报,2005,28(1):24-28.
• [12]WU Yu-xiang,GAO Yan-hui,ZHU Shui-jin,JI Dao-fan.Identifi-cation of SSR molecular marker on the interspecific hybrid F1 amongcultivated species in Gossypium[J].Journal of Zhejiang University:Agric.&Life Sci.,2007,33(1):56-60.吴玉香,高燕会,祝水金,季道藩.4个栽培棉种间四元杂种的SSR分子标记鉴定[J].浙江大学学报:农业与生命科学版,2007,33(1):56-60.
• [13]LIU Huan-fang,CHEN Xue-sen,DUAN Cheng-guo,YANG Hong-hua,FENG Bao-chun.Embryo rescue and identification of hybridsbetween sweet cherry and Chinese cherry[J].Acta HorticulturaeSinica,2004,31(3):303-308.刘焕芳,陈学森,段成国,杨红花,冯宝春.甜樱桃与中国樱桃杂种的胚抢救及杂种鉴定[J].园艺学报,2004,31(3):303-308.
• [14]DAI Lin-jian,LI Xun,GUAN Chun-yun,CHEN She-yuan,ZHONGJun.Regeneration and identification of intergeneric hybrid betweenbrassica napus and Eruca sativa[J].Chinese Journal of Oil Crop Sci-ences,2005,27(4):7-12.戴林建,李讯,官春云,陈社员,钟军.甘蓝型油菜与芸芥属间杂种F1的获得及鉴定[J].中国油料作物学报,2005,27(4):7-12.