赵瑞娟;薛华柏;王磊;温晶晶;杨健;王龙;王苏珂;苏艳丽;李秀根;

• 1:中国农业科学院郑州果树研究所

摘要(Abstract)：

【目的】筛选适合梨种质资源遗传多样性研究、品种鉴定分析的SSR核心引物。【方法】利用来源于不同系统、遗传背景差异较大的12份梨种质资源对已报道的800对梨和苹果的SSR引物和本课题组开发的534对SSR引物进行初步筛选,再利用48份不同地理来源的梨种质资源对初筛引物进行复筛,筛选一套适合梨种质资源遗传多样性研究、系统发育和品种鉴定分析的SSR核心引物,并通过遗传多样性分析和聚类分析验证引物的有效性。【结果】初筛出SSR引物131对,进一步复筛筛选出25对清晰稳定、多态性高的核心引物。25对核心引物对48份梨种质资源进行遗传多样性分析,共得到387个等位基因,平均每个位点等位基因数15.48个。各位点杂合度变幅为0.44~0.92,均值0.76;多态性信息含量变幅为0.70~0.92,均值0.85;基因多样性变幅为0.73~0.92,均值为0.87,说明引物的多态性高,能够有效揭示48份梨种质资源的遗传多样性。48份梨种质资源的聚类分析结果显示,西洋梨和东方梨分别聚成了2个类群,东方梨类群中的栽培种和野生种分别聚成了2个亚群;栽培种中的秋子梨品种聚在了一起,白梨和砂梨聚在了一起。【结论】筛选出的25对梨SSR引物,带型清晰、稳定,多态性信息含量均在0.70以上,可作为核心引物用于梨种质资源鉴定和遗传多样性分析等研究。

关键词(KeyWords)： 梨;SSR核心引物;遗传多样性;聚类分析

Abstract:

Keywords:

基金项目(Foundation): 中国农业科学院科技创新工程(CAAS—ASTIP);; 中央级科研院所基本科研业务费专项(1610192016612、1610192017709);; 现代农业(梨)产业技术体系(CARS-29)

作者(Author): 赵瑞娟;薛华柏;王磊;温晶晶;杨健;王龙;王苏珂;苏艳丽;李秀根;

Email:

DOI: 10.13925/j.cnki.gsxb.20170099

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