张云飞;李晓娇;岳建苏;江高飞;冉春;

• 1:西南大学园艺园林学院·南方山地园艺学教育部重点实验室
• 2:中国农业科学院柑桔研究所·国家柑桔工程技术研究中心
• 3:西南大学植物保护学院

摘要(Abstract)：

【目的】为明确γ氨基丁酸受体基因与橘全爪螨抗性的关系,【方法】通过BLAST搜索,对GABA受体基因进行鉴定;进一步通过序列比对和Sanger测序,对橘全爪螨敏感品系和噻螨酮抗性品系GABA受体基因SNPs进行分析和真实性验证;采用RPKM法对橘全爪螨敏感品系和噻螨酮抗性品系GABA受体基因进行表达差异分析。【结果】从橘全爪螨转录组中获得了19条GABA受体基因。通过敏感品系和抗性品系GABA受体基因序列比较和sanger测序发现Unigene11199_All有2个SNP位点。对GABA受体基因表达差异分析发现,相对于敏感品系,抗性品系中部分GABA受体基因表达量发生了不同程度的变化,抗性品系中有3条GABA受体基因表达上调,13条GABA受体基因表达下调,Unigene24440_All下调倍数最高[log2 Ratio(RS/SS)=-10.452479]。【结论】由此推断,橘全爪螨抗性产生可能是一个比较复杂的过程,而Unigene11199_All和Unigene24440_All可能是橘全爪螨对噻螨酮产生抗性的重要原因。

关键词(KeyWords)： 橘全爪螨;抗性;GABA受体基因;差异分析;噻螨酮

Abstract:

Keywords:

基金项目(Foundation): 国家科技支撑计划课题(2012BAD19B06);; 长江学者和创新团队发展计划项目(IRT0976);; 柑桔学重庆市市级重点实验室开放基金计划资助项目

作者(Author): 张云飞;李晓娇;岳建苏;江高飞;冉春;

Email:

DOI: 10.13925/j.cnki.gsxb.2012.05.004

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