张睿佳;李瑛;虞秀明;娄玉穗;许文平;张才喜;赵丽萍;王世平;

• 1:上海交通大学农业与生物学院

摘要(Abstract)：

【目的】探讨高温胁迫和外源油菜素内酯对鲜食葡萄光合和果实品质的影响。【方法】以3 a(年)生盆栽‘巨峰’葡萄为试材,连续高温胁迫20 d并喷施外源油菜素内酯(BR),测定葡萄叶片光合速率、叶绿素荧光参数、叶绿素含量及果实品质指标的变化。【结果】高温处理3 d即可显著降低叶片的净光合速率(Pn)、叶绿素含量、PSII实际光化学效率(ΦPSII)和光化学猝灭系数(q P),而初始荧光(Fo)显著高于对照。连续高温处理6 d,PSII最大光化学效率(Fv/Fm)开始下降。高温胁迫显著降低了果实的单粒质量、可滴定酸含量和果皮花色苷含量。高温处理20 d后,喷施BR的处理分别比未喷施处理的光合最大速率Amax、Fv/Fm、ΦPSII、q P和叶绿素含量均有提高,果皮花色苷含量为未喷施BR处理组的2.1倍。【结论】连续的高温胁迫会降低PSII反应中心活性,阻碍PSII的正常功能,抑制葡萄叶片的光合作用,影响果实品质。喷施外源BR可以有效缓解高温对葡萄叶片的光合伤害并促进果实着色。

关键词(KeyWords)： ‘巨峰’葡萄;高温胁迫;油菜素内酯;光合作用;果实品质

Abstract:

Keywords:

基金项目(Foundation): 国家葡萄产业技术体系资助(CARS-30-zp-07);; 上海果业产业技术体系(沪农科(2014)第7号)

作者(Author): 张睿佳;李瑛;虞秀明;娄玉穗;许文平;张才喜;赵丽萍;王世平;

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参考文献(References)：

• [1]MITTLER R.Abiotic stress,the field environment and stress combination[J].Trends in Plant Science,2006,11(1):15-19.
• [2]RIZHSKY L,LIANG H,SHUMAN J,SHULAEV V,DAVLETOVA S,MITTLER R.When defense pathways collide:The response of Arabidopsis to a combination of drought and heat stress[J].Plant Physiology,2004,134:1683-1696.
• [3]KRISHNA P S.Plant responses to heat stress[M]//Plant Responses to Abiotic Stress.Springer Berlin Heidelberg,2004:73-101.
• [4]BANOWETZ G M,AZEVEDO M D,EL-NASHAAR H M,MARTIN R C,STOUT R G.Temperature-induced increase in cellular chelating potential associated with reduced thermotolerance[J].Journal of Thermal Biology,2007,32:12-19.
• [5]GONG Zhiqiang,WANG Yanjiao,WANG Zunya,MA Lijuan,SUN Chenghu,ZHANG Siqi.Briefly analysis on climate anomalies and causations in summer 2013[J].Meteorological Monthly,2014,40(1):119-125.龚志强,王艳娇,王遵娅,马丽娟,孙丞虎,张思齐.2013年夏季气候异常特征及成因简析[J].气象,2014,40(1):119-125.
• [6]SUN Yongjiang,FU Yandong,DU Yuanpeng,ZHAI Heng.Effects of different light intensity on PSII activity and recovery of Vitis vinifera cv.Cabernet Sauvignon leaves under high temperature stress[J].Plant Physiology Journal,2014,50(8):1209-1215.孙永江,付艳东,杜远鹏,翟衡.不同温度/光照组合对‘赤霞珠’葡萄叶片光系统II功能的影响[J].中国农业科学,2014,50(8):1209-1215.
• [7]LUO H B,MA L,XI H F,DUAN W,LI S H,LOESCHER W,WANG J F,WANG L J.Photosynthetic responses to heat treatments at different temperatures and following recovery in grapevine(Vitis amurensis L.)leaves[J].Plos One,2011,6(8):e23033.
• [8]LU Xiaomin,YANG Wei.Alleviation effects of brassinolide on cucumber seedlings under Na Cl stress[J].Chinese Journal of Applied Eeology,2013,24(5):1409-1414.陆晓民,杨威.油菜素内酯对氯化钠胁迫下黄瓜幼苗的缓解效应[J].应用生态学报,2013,24(5):1409-1414.
• [9]WU Xuexia,ZHA Dingshi,ZHU Zongwen,XU Shuang.Effects of exogenous 24-Epibrassinolide on plant growth and antioxidant system in eggplant seedlings under high temperature stress[J].Plant Physiology Journal,2013,49(9):929-934.吴雪霞,查丁石,朱宗文,许爽.外源24-表油菜素内酯对高温胁迫下茄子幼苗生长和抗氧化系统的影响[J].植物生理学报,2013,49(9):929-934.
• [10]LI Jing,WANG Qiaoqiao,XU Nianjun,SUN Xue,FAN Meihua.Effects of 24-epibrassinolide on the resistance of Gracilaria lemaneiformis to high temperature[J].Acta Oceanologica Sinica,2014,36(8):82-90.李静,王俏俏,徐年军,孙雪,范美华.2、4-表油菜素内酯对龙须菜抗高温胁迫的研究[J].海洋学报,2014,36(8):82-90.
• [11]WANG S,OKAMOTO G,HIRANO K,LU J,ZHANG C.Effects of restricted rooting volume on vine growth and berry development of‘Kyoho’grapevines[J].American Journal of Enology and Viticulture 2001,52(3):250-255.
• [12]ZHENG Qiuling,TAN Wei,MA Ning,ZHAI Heng.Effects of calcium on photosynthesis and chlorophyll fluorescence of‘Kyoho’grape under high temperature stress[J].Scientia Agricultura Sinica,2010,43(9):1963-1968.郑秋玲,谭伟,马宁,翟衡.高温下巨峰葡萄叶片光合作用和叶绿素荧光的影响[J].中国农业科学,2010,43(9):1963-1968.
• [13]YANG Fuchen,WU Jiang,CHENG Jianhui,XU Kai,CHEN Junwei.Studies on extraction and physical-chemical properties of anthocyanin from Red Globe grape peel[J].Journal of Fruit Science,2007,24(3):287-292.杨夫臣,吴江,程建徽,徐凯,陈俊伟.葡萄果皮色素的提取及其理化性质[J].果树学报,2007,24(3):287-292.
• [14]SOLOMON S.Climate change 2007-the physical science basis:working Group I Contribution to the Fourth Assessment Report of the IPCC[R].Cambridge University Press:Cambridge,UK,2007:851.
• [15]GREER D H,WEEDON M M.Modelling photosynthetic responses to temperature of grapevine(Vitis vinifera cv.Semillon)leaves on vines grown in a hot climate[J].Plant,Cell and Environment,2012,35:1050-1064.
• [16]FAN Ling,DUAN Wei,CHENG Jieshan,LI Shaohua,WANG Lijun,LIU Guojie.Effects of salicylic acid on photosynthetic apparatus PSII in leaves of young grape plants under heat stress and during the recovery period from stress[J].Journal of Fruit Science,2009,26(5):623-627.范苓,段伟,程杰山,李绍华,王利军,刘国杰.水杨酸对高温胁迫下及恢复期间葡萄幼苗叶片光合机构PSII的影响[J].果树学报,2009,26(5):623-627.
• [17]LIU Chao,YUAN Ye,GAI Shupeng,ZHANG Yuxi,LIU Chunying,ZHENG Guosheng.Effects of strong light coupled with high temperature treatment on energy transfer between PSII and PSI in tree peony leaves[J].Acta Horticulturae Sinica,2014,41(2):311-318.刘超,袁野,盖树鹏,张玉喜,刘春英,郑国生.强光高温交叉胁迫对牡丹叶片PSⅡ和PSⅠ之间能量传递的影响[J].园艺学报,2014,41(2):311-318.
• [18]ZHANG Jie,LI Tianlai,XU Jing.Effects of daytime sub-high temperature on greenhouse tomato growth,development,yield and quality[J].Chinese Journal of Applied Ecology,2005,16(6):1051-1055.张洁,李天来,徐晶.昼间亚高温对日光温室番茄生长发育、产量及品质的影响[J].应用生态学报,2005,16(6):1051-1055.
• [19]XIE Zhaosen,WANG Shiping,XU Wenping.Accumulation of sugars and their regulation in grape berries[J].Plant Physiology Communications,2008,44(4):785-790.谢兆森,王世平,许文平.葡萄果实中的糖分积累和调控[J].植物生理学通讯,2008,44(4):785-790.
• [20]CARBONELL-BEJERANO P,SANTA MARíA E,TORRESPéREZ R,ROYO C,LIJAVETZKY D,BRAVO G,AGUIRREOLEA J,SáNCHEZ-DíAZ M,ANTOLíN M C,MARTíNEZZAPATER J M.Thermotolerance responses in ripening berries of Vitis vinifera L.cv Muscat Hamburg[J].Plant and Cell Physiology,2013,54(7):1200-1216.
• [21]COHEN S D,TARARA J M,KENNEDY J A.Assessing the impact of temperature on grape phenolic metabolism[J].Analytica Chinica Acta,2008,621(1):57-67.
• [22]HUANG Jinghan,WEN Kerui,PAN Qiuhong,DUAN Changqing,WANG Jun.The effects of environmental conditions and cultivation techniques on grape anthocyanins biosynthesis[J].SinoOverseas Grapevine&Wine,2011(5):71-76.黄敬寒,温可睿,潘秋红,段长青,王军.环境条件和栽培技术对葡萄花色苷生物合成的影响(上)[J].中外葡萄与葡萄酒,2011(5):71-76
• [23]MORI K,GOTO-YAMAMOTO N,KITAYAMA M,HASHIZUME K.Loss of anthocyanins in red-wine grape under high temperature[J].Journal of Experimental Botany,2007,58:1935-1945.
• [24]AZUMA A,YAKUSHIJI H,KOSHITA Y,KOBAYASHI S.Flavonoid biosynthesis-related genes in grape skin are differentially regulated by temperature and light conditions[J].Planta,2012,236:1067-1080.
• [25]WAHID A,GELANI S,ASHRAF M,FOOLAD M R.Heat tolerance in plants:An overview[J].Environmental and Experimental Botany,2007,61:199-223.
• [26]LIU Haixia,LI Xiaoyan,LI Lianguo,WANG Lijun,LI Shaohua.Research on Grape’s physical response in high temperature stress[J].Inner Mongolia Agricultural Science and Technology,2007(2):68-70.刘海霞,李晓燕,李连国,王利军,李绍华.葡萄对高温胁迫的生理学响应研究进展[J].内蒙古农业科技,2007(2):68-70.
• [27]MA Lina,XI Zhumei,HUO Shanshan,LUAN Liying,GAO Xiang,ZHAO Xiaolin.Studies on the regulation of anthocyanin biosynthesis in grape berry by brassinosteroid and abscisic acid[J].Journal of Fruit Science,2012,29(5):830-836.马立娜,惠竹梅,霍珊珊,栾丽英,高翔,赵晓琳.油菜素内酯和脱落酸调控葡萄果实花色苷合成的研究[J].果树学报,2012,29(5):830-836.
• [28]SYNONS G M,DACIES C,SHAVRUKOV Y,DRY I B,REID J B,THONAS M R.Grapes on steroids.Brassinosteroids are involved in grape berry ripening[J].Plant Physiology,2006,140(1):150-158.
• [29]DE VLEESSCHAUWER D,VAN BUYTEN E,SATOH K,BALIDION J,MAULEON R,CHOI I,VERA-CRUZ C,KIKUCHI S,HFTE M.Brassinosteroids antagonize gibberellin-and salicylate-mediated root immunity in rice[J].Plant Physiology,2012,158(4):1833-1846.