张玲,林顺权,杨向晖

• 1:华南农业大学园艺学院

摘要(Abstract)：

【目的】为从枇杷果实中提取高质量的RNA,【方法】以枇杷果实为材料,对6种总RNA提取方法进行分析和比较。【结果】结果表明,在裂解前先用70%丙酮和80%乙醇分别对枇杷果皮和果肉进行预处理,再结合CTAB-LiCl法,得到的总RNA质量较好,OD260/OD280在1.8～2.2,OD260/OD230超过2.0,得率分别为幼果果皮56.78μg.g-1,幼果果肉40.62μg.g-1,成熟果果皮40.86μg.g-1,成熟果果肉9.24μg.g-1;并根据其他植物的八氢番茄红素合成酶基因(PSY)保守区设计引物,用以上方法提取的RNA为模板进行RT-PCR,得到453 bp的基因片段,其推导的氨基酸序列与其他植物的同源性高达95%,推测该片段为PSY基因。【结论】先用70%丙酮和80%乙醇分别对枇杷果皮和果肉进行预处理,再结合CTAB-LiCl法,得到的RNA质量好,可用于后续的分子生物学研究。

关键词(KeyWords)： 枇杷果实;总RNA提取;PSY基因

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30971836)

作者(Author): 张玲,林顺权,杨向晖

DOI: 10.13925/j.cnki.gsxb.2012.04.017

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