高婷;刘欣颖;张新忠;王忆;李天红;韩振海;

• 1:中国农业大学园艺植物研究所

摘要(Abstract)：

【目的】为分析苹果实生树对苹果腐烂病抗病性的遗传规律,【方法】2010—2011年连续2 a用2个苹果腐烂病菌株对‘紫塞明珠’ב富士’的杂种实生树1 150株进行离体接种鉴定,采用次数分布分析法研究了苹果腐烂病抗病性遗传。【结果】结果表明,连续2 a接种2菌株主基因遗传率均在78.5%～85.5%。该实生群体对菌株03-8的感病性的变异由5个主基因位点分离所致,而对菌株xc56的感病性分离与4个主基因位点有关,且年份间表现稳定。【结论】离体枝条接种病原后,发病/不发病表现为质量性状遗传,发病对不发病为显性。感病的实生树接种后发病的病斑长度表现为多基因数量性状遗传。

关键词(KeyWords)： 苹果;苹果腐烂病;遗传规律

Abstract:

Keywords:

基金项目(Foundation): 国家公益性行业(农业)科研专项(201003021);; 863课题(2011AA001204);; 国家现代苹果产业技术体系(CARS-28)

作者(Authors): 高婷;刘欣颖;张新忠;王忆;李天红;韩振海;

DOI: 10.13925/j.cnki.gsxb.2012.05.005

参考文献(References)：

• [1]WANG Xu-li,WEI Jie-ling,HUANG Li-li,KANG Zhen-sheng.Re-evaluation of pathogens causing Valsa canker on apple in China[J].Mycologia,2011,103(2):317-324.
• [2]CAO Ke-qiang,GUO Li-yun,LI Bao-hua,CHEN Han-jie.Inves-tigations on the occurrence and control of apple canker in China[J].Plant Protection,2009,35(20):114-116.曹克强,国立耘,李保华,陈汉杰.中国苹果树腐烂病发生和防治情况调查[J].植物保护,2009,35(20):114-116.
• [3]SAWAMURA K,HARADA Y,YUKITA K.Apple valsa cankercaused by Valsa ceratosperma(Tode:Fr.)maire[J].Bulletin of theFaculty of Agriculture,Hirosaki University,1990,53(33):10-32.
• [4]LIU Han-zhong,REN Qing-mian,LIU Li-jun.Research on the re-sistant of apple canker identification traits[J].Journal of Fruit Sci-ence,1990,7(2):65-70.刘捍中,任庆棉,刘立军.苹果属种质资源抗腐烂病性状鉴定研究[J].果树科学,1990,7(2):65-70.
• [5]ABE K,KOTODA N,KATO H,SOEJIMA J.Resistance sources toValsa canker(Valsa ceratosperma)in a germplasm collection of di-verse Malus species[J].Plant Breeding,2007,126(4):449-453.
• [6]BESSHO H,TSUCHIYA S,SOEJIMA J.Screening methods of appletrees for resistance to Valsa canker[J].Euphytica,1994,77:15-18.
• [7]LIU Xin-ying,LV Song,WANG Yi,WANG Kun,LI Tian-hong,HAN Zhen-hai,ZHANG Xin-zhong.Evaluation of resistance ofmalus germplasms to apple canker(Valsa ceratosperma)[J].Journalof Fruit Science,2011,28(5):843-848.刘欣颖,吕松,王忆,王昆,李天红,韩振海,张新忠.苹果种质资源对苹果树腐烂病抗性评价[J].果树学报,2011,28(5):843-848.
• [8]LIU Shu-xiao.Survey on resistance to Valsa canker in different applevarieties[J].Journal of Tianjin Agricultural College,1999,6(4):48-49.刘书晓.苹果不同品种对腐烂病的抗病性调查研究[J].天津农学院学报,1999,6(4):48-49.
• [9]ABE K,KOTODA N,KATO H,SOEJIMA J.Genetic studies on re-sistance to Valsa canker in apple:genetic variance and breeding val-ues estimated from intra-and inter-specific hybrid progeny popula-tions[J].Tree Genetics&Genomes,2011,7:363-372.
• [10]SUN Huan-huan,ZHAO Yong-bo,LI Chun-min,CHEN Dong-mei,WANG Yi,ZHANG Xin-zhong,HAN Zhen-hai.Identifica-tion of markers linked to major gene loci involved in determination offruit shape index of apples(Malus domestica)[J].Euphytica,DOI10.1007/s10681-011-0515-x.
• [11]DUREL C E,PARISI L,LAURENS F,VAN DE WEG W E,LIEB-HARD R,JOURJON M F.Genetic dissection of partical resistanceof Venturia inaequalis in apple[J].Genome,2003,46(2):224-234.
• [12]LIEBHARD R,KOLLER B,PATOCCHI A,KELLERHALS M,PFAMMATTER W,JERMINI M,GESSLER C.Mapping quantita-tive field resistance against apple scab in a‘Fiesta’בDiscovery’progeny[J].Phytopathology,2003,93:493-501.
• [13]CALENGE F,FAURE A,GOERRE M,GEBHARDT C,VAN DEWEG W.E,PARRISI L,DUREL C E.Quantitative trait loci(QTL)analysis reveals both broad-spectrum and isolate-specific QTL forscab resistance in an apple progeny challenged with eight isolates ofVenturia inaequalis[J].Phytopathology,2004,94:370-379.
• [14]QU Shen-chun,LV Dong,ZHANG Zhen.Research advances of re-sistant genes in apple[J].Journal of Agricultural Science and Tech-nology,2009,11(5):36-41.渠慎春,吕东,章镇.苹果抗病虫基因研究进展[J].中国农业科技导报,2009,11(5):36-41.
• [15]YAN Zhen-li,ZHANG Heng-tao,ZHANG Quan-jun,ZHANGShun-ni,GUO Guo-nan,LIU Zhen-zhen.Study on the inheritanceof apple resistance to ring spot disease[J].Journal of Henan Agri-cultural Sciences,2009,8:105-108.阎振立,张恒涛,张全军,张顺妮,过国南,刘珍珍.苹果抗轮纹病遗传的初步研究[J].河南农业科学,2009,8:105-108.
• [16]LIU Hai-tao,LI Cai-li,ZHAO Yong-bo,LI Chun-min,CHENDong-mei,YANG Feng-qiu,WANG Yi,XU Xue-feng,ZHANGXin-zhong,HAN Zhen-hai.Inheritance and hybrid primary selec-tion in breeding of resistance to apple bot canker[J].Jiangsu Agricu-tural Sciences,2011,39(2):241-244.刘海涛,李彩丽,赵永波,李春敏,陈东玫,杨凤秋,王忆,许雪峰,张新忠,韩振海.苹果育种杂种初选轮纹病抗性评价及遗传分析[J].江苏农业科学,2011,39(2):241-244.
• [17]ZHAO Lei,ZHAO Zheng-yang,DANG Zhi-guo,ZHANG Kun,ZHANG Xiao-cai.Heredity analysis on the hybrid of Qingguan andFuji apple resistance to early defoliation disease[J].Acta Agricultur-ae Boreali-occidentalis Sinica,2008,17(2):197-201.赵磊,赵政阳,党志国,张坤,张小猜.秦冠、富士苹果杂交后代早期落叶病的遗传分析[J].西北农业学报,2008,17(2):197-201.
• [18]LIU H T,LI C L,ZHANG Y J,LI C M,ZHAO Y B,CHEN D M,WANG Y,ZHANG X Z,HAN Z H.Inheritance and Molecularmarker of resistance to bot canker in Malus domestica[J].Journal ofIntegrative Agriculture,2011,10(2):175-184.
• [19]ZHUANG Yan,LIU Hai-tao,LI Chun-min,WANG Yi,ZHAOYong-bo,CHEN Dong-mei,HAN Zhen-hai,ZHANG Xin-zhong.Inheritance of and molecular markers for susceptibility of Malus do-mestica to fruit ring rot(Botryosphaeria dothidea)[J].Journal of Phy-topathology,2011,159:782-788.
• [20]SUZAKI K.Population structure of Valsa ceratosperma,causal fun-gus of Valsa canker,in apple and pear orchards[J].Journal of GeneralPlant Pahtology,2008,74:128-132.