王建辉;刘建军;陈克玲;李洪雯;何建;关斌;

• 1:四川省农业科学院园艺研究所
• 2:农业部西南地区园艺作物生物学与种质创制重点实验室
• 3:四川省农业科学院

摘要(Abstract)：

【目的】为了研究不同葡萄病毒病快速检测方法和四川分离株系统进化,【方法】比较3种葡萄总RNA提取方法,还优化了葡萄病毒病的RT-PCR检测方法。分别克隆了3种病毒四川分离株的部分基因组区域,并开展了系统进化分析。【结果】结果表明,改进硅胶颗粒吸附法快速从葡萄枝条的韧皮组织中提取了总RNA,并以葡萄18SRNA为内参基因,RT-PCR分别成功扩增出3种病毒的目的片段。把16个地理起源的GLRaV-2分离株的外壳蛋白基因(Coat protein,CP)分成5个系统进化组。而7个地理起源的GVB分离株CP同源性为79.1%～98.7%。此外,5个GVA四川分离株分别位于系统进化树的4个聚类群。【结论】GLRaV-2、GVB和GVA不同地理起源的分离株在核酸水平上具有变异性,其中GVA四川分离株之间具有显著的遗传差异,可能包括4种株系。

关键词(KeyWords)： 葡萄卷叶伴随2型病毒;葡萄B病毒;葡萄A病毒;病毒外壳蛋白基因;病毒沉默抑制子基因

Abstract:

Keywords:

基金项目(Foundation): “十二五”四川省财政基因工程:果树生物技术研究与创新(2011JYGC05-018)

作者(Authors): 王建辉;刘建军;陈克玲;李洪雯;何建;关斌;

DOI: 10.13925/j.cnki.gsxb.2013.02.002

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