植物生长调节剂对‘长富—2’苹果离体繁殖的效应EFFECTS OF PLANT GROWTH REGULATOR ON IN VITRO PROPAGATION OF APPLE CULTIVAR'FUJI'
马锋旺,王居仓,雍文
摘要(Abstract)：
植物生长调节剂对‘长富—2’苹果离体繁殖的效果表明:(1)增殖和生长以BA 1.0—1.5mg/L、IBA 0.5mg/L 较为适宜,高浓度的 BA 和 IAB 易产生玻璃苗和其它异常现象;赤霉素促进新梢的生长.浓度为3.0mg/L 时对增殖有促进作用.(2)三十烷醇对增殖和生长具有显著的效应.(3)不定根的诱导以附加1.0—3.0mg/L IAA 或0.5mg/L IBA 较好.(4)培养基中附加 PP333可以促进根的形成。提高试管苗的移栽成活率.(5)转移培养对生根率和生根数目无显著影响,但有利于试管苗的移栽.
关键词(KeyWords)： 苹果;‘长富—2’;生长调节剂;离体繁殖
基金项目(Foundation):
作者(Author): 马锋旺,王居仓,雍文
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参考文献(References)：
- (1) 陈善坤,1984． 三十烷醇专题考察报告。植物生理学通讯,(1) :73-75。
- (2) 费开伟,薛光荣:1980,苹果金冠品种的茎尖培养。中国农科院果树研究所研究年报,105-106。
- (3) 李颖章(译):1984,作为植物生长调节剂的三十烷醇.植物生理学通讯,(4) :5(?)-68。
- (4) 刘世峰等:1978． 苹果矮化砧木和苹果实生苗的茎尖培养.植物学报,20(4) :337-340．
- (5) Lundergan,C.A.et al(周清桂译):1981,苹果幼茎离体增殖和生长的调节.国外农学-果树,(2) ,8-11．
- (6) Brainerd,K.E.and Fuchigami,L.H.:1981,Acclimatization of aseptically cultured apple plants to low relative humidity.J.Amer.Soc.Hort.Sci,106(4) ,515-518．
- (7) James,D.J.and Thurbon,I.J.:1979． Rapid in vutro rooting of the apple rootstock M9． Journal of Horticultural Science,54(4) ,309-311．
- (8) James,D.J.:1983． Adventitious root formation in vitro in apple rootstock:Ⅰ.Factors affecting the length of the auxin-sensitive phase in M9． Physiol.Plant,57,149-153．
- (9) Lane,W.D.:1978． In vitro propagation of apple using shoot tip culture.Plant Science Letters,13:281-285．
- (10) Pua,E.C.et al:1983． In vitro propagation of Ottawa 3 apple rootstock.Can.J.Plant Sci,63,183-188．
- (11) Swietlik,D.and Miller.S.S.:1983． The effect of paclobutrazol on growth and response to water stress of apple seedlings.J.Amer.Soc Hort.Sci,108(6) :1076-1080．
- (12) Welander,M.:1985． in vitro shoot and root formation in the apple cultivar AKero.Annal of Botany,55:249-261．
- (13) Zimmerman,R.H.:1984． Rooting apple cultivars in vitro,Interactions among light,temperatu(?).phloroglucinol and auxin.Plant Cell Tissue Organ Culture,3:301-311．