秦国新;何桥;梁国鲁;雷家军;郭启高;

• 1:西南大学园艺园林学院
• 2:山西农业大学园艺学院
• 3:沈阳农业大学园艺学院

摘要(Abstract)：

为利用SCoT技术开展草莓属(Fragaria)植物种质资源遗传多样性及分子系统学研究,以草莓属植物11个野生种和1个栽培品种为试材,采用L25(56)正交试验设计,建立了草莓属植物的最优SCoT-PCR反应体系,即20μL反应体系中,含Mg2+2.0 mmol·L-1、dNTPs 0.3 mmol·L-1、Taq酶0.75 U、引物0.75μmol·L-1、模板DNA 40 ng,并对退火温度进行了优化,对电泳检测方法及草莓属植物DNA提取方法进行了改进。共筛选出22个适于进行草莓属植物SCoT分析的引物。经验证,该反应体系稳定性好、可重复性强、多态性高、扩增效果好,适于草莓属植物的SCoT分析。

关键词(KeyWords)： 草莓属;SCoT;体系优化

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30971976)

作者(Author): 秦国新;何桥;梁国鲁;雷家军;郭启高;

Email:

DOI: 10.13925/j.cnki.gsxb.2012.03.009

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