王卓;徐碧玉;贾彩红;李健平;刘菊华;张建斌;苗红霞;金志强;

• 1:中国热带农业科学院热带生物技术研究所农业部热带生物技术重点开放实验室
• 2:中国热带农业科学院海口实验站

摘要(Abstract)：

【目的】获得香蕉抗逆相关转录因子。【方法】通过随机克隆测序的方法从香蕉根系c DNA文库中获得MYB基因,命名为Ma MYB1。【结果】对扩增获得的c DNA序列进行生物信息学分析,表明Ma MYB1是香蕉MYB基因编码框全长c DNA,包含一个951 bp的最大开放阅读框,编码一个长316个氨基酸的蛋白质。蛋白质序列同源比对发现其含有完整的2个保守的MYB结构域R2和R3,属于典型的R2R3-MYB基因。系统进化树比对分析表明,Ma MYB1与海枣(XP_008808341.1)和油棕(XP_010914123.1)的亲缘关系较近。组织特异性研究表明该基因在叶、根和花中的表达量较高。实时荧光定量PCR分析表明Ma MYB1响应干旱、低温、盐和枯萎病菌的侵染等胁迫处理。【结论】Ma MYB1基因在香蕉响应逆境中扮演重要的调控角色。

关键词(KeyWords)： 香蕉;MYB1;基因克隆;表达分析

Abstract:

Keywords:

基金项目(Foundation): 中央级公益性科研院所基本科研业务费专项资金“香蕉抗枯萎病全基因组关联分析”(ITBB2015ZY15);; 国家现代农业产业体系项目“国家香蕉产业技术体系”(CARS-32)

作者(Author): 王卓;徐碧玉;贾彩红;李健平;刘菊华;张建斌;苗红霞;金志强;

Email:

DOI: 10.13925/j.cnki.gsxb.20150095

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