代红艳;闫玉娇;李晓明;李贺;张志宏;

• 1:沈阳农业大学园艺学院
• 2:沈阳农业大学生物科学技术学院

摘要(Abstract)：

【目的】从山楂(Crataegus pinnatifida)中克隆过氧化物酶(POD)编码基因,通过转基因验证其在木质素合成中的作用,为揭示山楂内果皮形成分子机制奠定基础。【方法】利用RT-PCR技术从山楂克隆基因,以p RI 101-AN为构建基因过量表达载体的基础载体,通过农杆菌介导的遗传转化方法将目的基因导入烟草。【结果】从山楂中克隆出编码序列长度为996 bp的POD72基因,命名为Cp POD72。山楂POD72与木本和草本植物POD72的氨基酸序列一致性均较高。构建了Cp POD72基因的过量表达载体,通过农杆菌介导的转化获得了66个烟草转化植株。RT-PCR检测结果显示Cp POD72基因在烟草转基因植物中异位表达,组织化学染色分析表明转Cp POD72基因烟草植株中木质素含量增加。【结论】Cp POD72基因可能参与木质素合成。

关键词(KeyWords)： 山楂;过氧化物酶基因;木质素;转基因烟草;异位表达

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31170635)

作者(Author): 代红艳;闫玉娇;李晓明;李贺;张志宏;

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DOI:

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