陈晶晶;段雅婕;莫亿伟;胡玉林;胡会刚;谢江辉;

• 1:中国热带农业科学院南亚热带作物研究所·农业部热带果树生物学重点实验室
• 2:绍兴文理学院生命科学学院
• 3:中国热带农业科学院南亚热带作物研究所·国家热带果树种质资源圃

摘要(Abstract)：

【目的】探讨细胞壁代谢相关基因在裂果品种(AP番荔枝)和不裂果品种(PO番荔枝)采后贮藏期间的表达差异。【方法】对番荔枝2个品种采后后熟不同阶段以及对AP番荔枝进行喷施乙烯利处理后对果皮进行取样,测定果实硬度和可溶性固形物含量,利用实时荧光定量PCR技术检测8个细胞壁代谢相关基因在2个品种室温贮藏期间和乙烯利处理后,基因在不同阶段的表达变化,并应用CLUSTER软件对基因的差异表达进行双向层次聚类分析。【结果】AP番荔枝和PO番荔枝采后后熟期间的可溶性固形物含量和硬度值变化基本一致,2者之间无明显差异,而乙烯利处理能加速AP番荔枝的成熟与开裂。定量PCR分析表明,贮藏期间8个基因在PO番荔枝中的表达量均低于AP番荔枝,其中PPO在PO番荔枝中无表达,EXP2、EXP3和PE在PO番荔枝贮藏期前3 d表达量很低。EXP1在2个番荔枝品种贮藏期间一直处于高水平表达,但不受外源乙烯诱导,而EXP2、EXP3、XET1、XET3、PE和PPO均不同程度受到乙烯诱导。大多数基因在第1天,果实开裂前表达量已增加,随着裂果的加重表达量下降。聚类分析表明在PO番荔枝后熟过程中发生剧烈变化的有EXP1、EXP2、EXP3和PE基因,在AP-正常和AP-乙烯裂果前后表达量变化比较剧烈的基因基本一致,包括EXP1、EXP2、EXP3、PPO和PE。【结论】EXP1更多和果实成熟软化有关。EXP2、EXP3、PE和PPO与裂果关系较为密切,而XET1、XET2和XET3可能与果实成熟软化有一定关系,但不是果实开裂的关键基因。

关键词(KeyWords)： 番荔枝;裂果;细胞壁代谢;基因表达

Abstract:

Keywords:

基金项目(Foundation): 中央级公益性科研院所基本科研业务专项(No.1630062013005);; 海南省自然科学基金项目(No.314109);; 国家香蕉产业技术体系湛江试验站(CARS-32-12)

作者(Authors): 陈晶晶;段雅婕;莫亿伟;胡玉林;胡会刚;谢江辉;

DOI: 10.13925/j.cnki.gsxb.20150234

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