梨成熟花药中生长调节物质及矿质元素含量的分析Analysis of growth regulative substances and mineral nutrients in mature anther of pears
李六林;张绍铃;
摘要(Abstract):
以丰水和新高梨为试材,分析了花药内花粉量和花粉的萌发特性,并在即将开花的花蕾期,对其花药中不同种类和形态的多胺、生长素、脱落酸以及矿质元素进行分析,以探讨蕾期花粉生活力高的原因。结果表明:丰水梨每个花药中有大量花粉,并且花粉的萌发率高,达到84.8%,而新高中花粉非常少,并且生活力非常低;丰水梨花药中腐胺、亚精胺和精胺的总量均极显著地高于新高;而且丰水中不同形态(游离态、高氯酸可溶共价结合态高氯酸不溶共价结合态)的3种多胺也都显著或极显著地高于新高;生长素、脱落酸与多胺表现出同样的规律;丰水矿质营养中的钾、钙、镁、铁、铜和锌含量也高于新高,在这些元素中以钾含量为最高,微量元素中以锌含量为最高。
关键词(KeyWords): 梨;花药;多胺;激素;矿质营养
基金项目(Foundation): 国家自然科学基金项目(30170651;2011030712)
作者(Author): 李六林;张绍铃;
Email:
DOI:
参考文献(References):
- [1]XU J Z,CHEN H J,SHAO J Z,et al(徐继忠,陈海江,邵建柱,等).Effects of exogenous polyamines on flower bud formation of Red Fuji apple variety[J].Journal of Fruit Science(果树科学),1998,15(1):10 ̄12.(in Chinese)
- [2]ZHANGSL,GAOF Y,CHENDX,et al(张绍铃,高付永,陈迪新,等).The effects of plant growth regulating substances on pollen germination and tube gowth in Housui pear[J].Acta Bot Boreal-Oc-cident Sin(西北植物学报),2003,23(4):586 ̄591.(in Chinese)
- [3]ZENGX(曾骧).Fruit tree physiology(果树生理学)[M].Beijing:Bei-jingAgriculture universityPress,1990:189 ̄192.(in Chinese)
- [4]ZHANG S L,XIE W N,CHEN D X,et al(张绍铃,谢文暖,陈迪新,等).The differences ofpollen content and pollen germination and tube growth among eight species of fruit trees[J].Acta Agriculturea Shanghai(上海农业科学报),2003,19(3):67 ̄69.(in Chinese)
- [5]LIUJ,JI X J,LIU Y L(刘俊,吉晓佳,刘友良).HPLC method for the identification and quantization of polyamines[J].Plant Physiology Communication(植物生理学通讯),2002,38(6):596 ̄598.(in Chinese)
- [6]KOTZAHASIS K,CHRISTAKIS-HAMPSAS MD,RONBELAKIS-ANGELAKISK A.A narrow-bore HPLC method for the identifica-tion and quantization of free conjugated and bound polyamines[J].Analytical Biochem,1993,214:484 ̄489.
- [7]WUSR,CHENW F,ZHOU X(吴颂如,陈婉芬,周燮).Enzyme linked immuno-sorbent assay for endogenous plant hormones[J].Plant PhysiologyCommunication(植物生理学通讯),1988(5):53 ̄57.(in Chinese)
- [8]TONGYA,ZHOUH J(仝月奥,周厚基).Fruit tree nutrient diag-nose(果树营养诊断)[M].Beijing:Agricultural press,1982:77 ̄102.(in Chinese)
- [9]KAKKAR R K,RAI V K.Plant polyamines in flowering and fruit ripening[J].Phytochem,1993,33(6):1281 ̄1288.
- [10]WANGSP,SONGCB,LI LC,et al(王世平,宋长冰,李连朝,等).Physiological function of polyamines during blossoming and fruit setting in apple[J].Acta Horticulturae Sinica(园艺学报),1996,23(4):319~325.(in Chinese)
- [11]FLORES H E,FILNER P.Polyamine catabolism in higher plants:characterization of pyrroline dehyrogenase[J].Plant Growth Regul,1985(3):277 ̄291.
- [12]RABITIAL,BETTIL,BORTOLOTTIG,etal.Shout-termpolyamine response in TMV-Inoculated hypersensitive and susceptible tobacco plants[J].NewPhytol,1998,139:549 ̄553.
- [13]HOHELFELDH,SCHURMUMW.Partlypurification and character-izationofhydroxycinnamic-coenzymeA[J].Plant Physiol,1995,107:545 ̄560.
- [14]SUN D Y,CHENG G.Extra cellular CaMaccelerates the prolifera-tion of suspension cultured cells of Angelica dahurica[J].Plant Sci-ence,1994,99:l ̄8.
- [15]BUSH D S.Calciumregulation in plant cells and its role in signaling[J].Annu RevPlant Mol Biol,1995,46:95 ̄112.
- [16]VALLEE B L,AULD D S.Zinc coordination,function and structure ofzinc enzymes and other Proteins[J].Biochemistry,1990,29:5647 ̄5659.
- [17]FALCHUK K H,HARDY C,ULPINO L,et al.RNA metabolism,manganese,and RNA Polymerases of zinc sufficient and zinc defi-cient Euglena gracili[J].Proc Nat Acad Sci.USA,1978,75:4175 ̄4179.
- [18]KOBAYASHI A,SAKAMOTO A,KUBO K,et al.Seven zinc-fin-ger transcription factors are expressed sequentially during the devel-opment ofanthers in Petunia[J].Plant,1998,13:571 ̄576.
- [19]BALTZ R,DOMON C,DATTA T,et al.Characterization of a pollen-specific cDNA from Sunflower encoding a zinc finger protein[J].Plant,1992,2(5):713~721.