刘艳丽;何绍兰;吕强;易时来;谢让金;郑永强;刘雪峰;邓烈;

• 1:西南大学园艺园林学院
• 2:西南大学
• 3:中国农业科学院柑桔研究所
• 4:国家柑桔工程技术研究中心

摘要(Abstract)：

【目的】试验以枳橙砧‘卡拉卡拉红肉脐橙’花器为试材,利用高光谱技术对柑橘花钾素营养进行表征,以期为柑橘植株营养的信息化管理提供理论依据。【方法】探索通过单波长阈值分割法提取柑橘花高光谱图像信息,利用PLS建模筛选对柑橘花钾素含量响应最优光谱数据,以此数据建立i PLS和si PLS的钾素含量估测模型,筛选出特征波段及组合,建立柑橘花钾营养的高光谱估测技术。【结果】采用花朵高光谱扫描信息,设置670.66 nm光谱0.5~0.6灰度区间可以较好提取到柑橘花组织有效光谱信息,筛选出558.17~612.16 nm特征波长和558.17~612.16 nm、612.16~666.69nm、666.69~721.69 nm特征波段区间组合,柑橘花钾素含量估测si PLS模型的预测效果最佳,其校正集相关系数和均方根误差分别为0.954 7和0.089 0,预测集相关系数和均方根误差也分别达0.885 5和0.161 9。【结论】建立的柑橘花钾素含量估测有效信息提取方法、特征光谱以及最优预测模型,为柑橘花钾素营养实时检测奠定了基础。

关键词(KeyWords)： 柑橘花;钾素含量;高光谱图像;特征波段;联合区间偏最小二乘法

Abstract:

Keywords:

基金项目(Foundation): 国家863计划课题(2012AA101904);; 国家国际科技合作专项(2013DFA11470);; 重庆市国际科技合作项目(cstc2011gjhz80001);; 重庆市科技攻关项目(cstc2012gg-yyjs80002);; 国家星火计划项目(2012GA811001)

作者(Author): 刘艳丽;何绍兰;吕强;易时来;谢让金;郑永强;刘雪峰;邓烈;

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参考文献(References)：

• [1]ZHENG Huan,REN Jun-peng,JIA Yue,ZHANG Zhen,TAO Jian-min.Changes of phytohormone contents during anther abortion of male sterile new grapevine selection‘2004-6-12’[J].Journal of Fruit Science,2012,29(3):350-354.郑焕,任俊鹏,贾玥,章镇,陶建敏.葡萄雌能花新种质(‘2004-6-12’)花蕾发育过程中植物内源激素的变化[J].果树学报,2012,29(3):350-354.
• [2]ZHU Xi-cun,ZHAO Geng-xing,WANG Ling,DONG Fang,LEI Tong,ZHAN Bing.Hyperspectrum based prediction model for nitrogen content of apple flowers[J].Spectroscopy and Spectral Analys,2010,30(2):416-420.朱西存,赵庚星,王凌,董芳,雷彤,战兵.基于光谱的苹果花氮素含量预测模型研究[J].光谱学与光谱分析,2010,30(2):416-420.
• [3]ZHOU Li-li,FENG Han-yu,YAN Zhong-min,LIU Ke,ZHOU Shun-li.Hyperspectral diagnosis of leaf N concentration of maize and variety difference[J].Transactions of the Chinese Society of Agricultural Engineering,2010,26(8):195-199.周丽丽,冯汉宇,阎忠敏,刘克,周顺利.玉米叶片氮含量的高光谱估算及其品种差异[J].农业工程学报,2010,26(8):195-199.
• [4]WANG Lei,BAI You-lu.Correlation between corn leaf spectral reflectance and leaf total nitrogen and chlorophyll content under different nitrogen level[J].Scientia Agricultura Sinica,2005,38(11):2268-2276.王磊,白由路.不同氮处理春玉米叶片光谱反射率与叶片全氮和叶绿素含量的相关研究[J].中国农业科学,2005,38(11):2268-2276.
• [5]XIE Rui-zhi,ZHOU Shun-li,WANG Ji-hua,JIANG Hai-rong.Comparison of the leaf hyperspectral reflectance and absorptance in relation to the leaf piament contents in corn plants[J].Journal of Maize Science,2006,14(3):70-73.谢瑞芝,周顺利,王纪华,蒋海荣.玉米叶片高光谱反射率、吸收率与色素含量的关系比较分析[J].玉米科学,2006,14(3):70-73.
• [6]MUHAMMAD H H.Hyperspectral crop reflectance data for characterising and estimating fungal disease severity in wheat[J].Biosystems Engineering,2005,91(1):9-20.
• [7]YANG X H,HUANG J F,WANG F M,WANG X Z,YI Q Q,WANG Y.A modified chlorophyll absorption continuum index for chlorophyll estimation[J].Journal of Zhejiang University SCIENCE A,2006,7(12):2002-2006.
• [8]CHENG Q,HUANG J F,WANG X Z,WANG R C.Insitu hyperspectral data analysis for pigment content estimation of rice leaves[J].Journal of Zhejiang University Science,2003,4(6):727-733.
• [9]LIN Fen-fang,CHEN Zhu-lu,WANG Ke,DENG Jin-song,XU Hong-wei.Determination of nitrogen in rice leaf based on FTIR spectra and si PLS-GA-PLS algorithm[J]Journal of Infrared andMillimeter Waves,2009,28(4):277-280.林芬芳,陈祝炉,王珂,邓劲松,许红卫.基于傅里叶变换红外光谱和si PLS-GA-PLS的水稻叶片氮素含量预测研究[J].红外与毫米波学报,2009,28(4):277-280.
• [10]LIU Liang-yun,JIN Zhi-wei,WANG Ji-hua,YANG Tie-zhao,HUANG Wen-jiang,ZHAO Chun-jiang.Prediction of nicotine,potassium and nitrogen in Tobacco with spectrometry[J].Tobacco Science&Technology/Tobacco Chemistry,2005,49(6):26-29.刘良云,靳志伟,王纪华,杨铁钊,黄文江,赵春江.光谱法预测烟叶中的烟碱、钾和氮素[J].烟草科技,2005,49(6):26-29.
• [11]QIAO Xin,MA Xu,ZHANG Xiao-chao,LIANG Liu-suo.Response of coronary spectrum on chlorophyll and K information of soy[J].Transactions of the Chinese Society for Agricultural Machinery,2008,39(4):108-111.乔欣,马旭,张小超,梁留锁.大豆叶绿素和钾素信息的冠层光谱响应[J].农业机械学报,2008,39(4):108-111.
• [12]YI Shi-lai,DENG Lie,HE Shao-lan,ZHENG Yong-qiang,ZHANG Xuan.Research on nitrogen content of leaf of Jincheng orange cultivap using visble nwar infrared spectroscopy model[J].Journal of Fruit Science,2010,27(1):13-17.易时来,邓烈,何绍兰,郑永强,张璇.锦橙叶片氮含量可见近红外光谱模型研究[J].果树学报,2010,27(1):13-17.
• [13]XING Dong-xing,CHANG Qing-rui.Research on predicting the TN,TP,TK contents of fresh fruit tree leaves by spectral analysis with red Fuji apple tree as an example[J].Journal of northwest A&F University:Natural Science Edition,2009,37(2):141-147.邢东兴,常庆瑞.基于光谱分析的果树叶片全氮、全磷、全钾含量估测研究—以红富士苹果树为例[J].西北农林科技大学学报:自然科学版,2009,37(2):141-147.
• [14]XING Dong-xing,CHANG Qing-rui.Ientification of species of fruit trees based on the spectral reflectance of canopies of fruit trees during flowering period[J].Journal of Infrared and Millimeter Waves,2009,28(3):207-211.邢东兴,常庆瑞.基于花期果树冠层光谱反射率的果树树种辨识研究[J].红外与毫米波学报,2009,28(3):207-211.
• [15]ZOU X B,ZHAO J W,LI Y X.Selection of the efficient wavelength regions in FT-NIR spectroscopy for determination of SSC of‘Fuji’apple based on Bi PLS and Fi PLS models[J].Vibrational Spectroscopy,2007,44(2):220-227.
• [16]CHAI Zhong-ping,WANG Xue-mei,SUN Xia,JIANG Ping-an,ZHANG Qian.Influence of N,P,K with drip irrigation on yield and fruit quality of Huizao jujube[J].Journal of Fruit Science,2011,28(2):229-233.柴仲平,王雪梅,孙霞,蒋平安,张谦.不同氮磷钾配比滴灌对灰枣产量与品质的影响[J].果树学报,2011,28(2):229-233.
• [17]XU Ye-ting,ZHANG Wen,YANG Bo,YANG Lei,GONG Peng,ZHANG Ping.Establishment and application of nutrient diagnosis system of‘Zhipi’almond leaf mineral elements in Shache County,Xinjiang[J].Journal of Fruit Science,2014,31(1):143-149.徐叶挺,张雯,杨波,杨磊,龚鹏,张平.新疆莎车‘纸皮’扁桃叶片营养诊断体系的建立与应用[J].果树学报,2014,31(1):143-149.
• [18]YI Shi-lai,DENG Lie,HE Shao-lan,ZHENG Yong-qiang,MAO Sha-sha.A spectrum based models for monitoring leaf potassium content of Citrus sinensis(L)cv.Jincheng Orange[J].Scientia Agricultura Sinica,2010,43(4):780-786.易时来,邓烈,何绍兰,郑永强,毛莎莎.锦橙叶片钾含量光谱监测模型研究[J].中国农业科学,2010,43(4):780-786.
• [19]JACKSON R D,JONES C A,UEHARA G,SANTO LT.Remotedetection of nutrient and water deficiencies in sugarcane under variable cloudiness[J].Remote Sensing of Environment,1980,11:327-331.
• [20]ZHAI Y F,CUI L J,ZHOU X,GAO Y,FEI T,GAO W X.Estimation of nitrogen,phosphorus and potassium contents in the leaves of different plants using laboratory-based visible and nearinfrared reflectance spectroscopy:comparison of partial leastsquare regression and support vector machine regression methods[J].International Journal of Remote Sensing,2013,34(7):2502-2518.
• [21]PONZONI F J,DE GONCALES J L M.Spectral features associated with nitrogen,phosphorusand kalium deficiencies in Eucalyptus saligna seedling leaves[J].International Journal of Remote Sensing,1999,20(11):2249-2264.
• [22]ZHU Xi-cun,ZHAO Geng-xing,LEI Tong.Reflective spectral characteristics of apple florescence canopy[J].Transactions of the CSAE,2009,25(12):180-186.朱西存,赵庚星,雷彤.苹果花期冠层反射光谱特征[J].农业工程学报,2009,25(12):180-186.
• [23]ZHU Xi-cun,ZHAO Geng-xing,SUI Xue-yan,LEI Tong,SUN Ding-guo.Estimation of kalium content in apple flowers based on spectral analysis technique[J].Infrared,2010,31(8):19-23.朱西存,赵庚星,隋学艳,雷彤,孙顶国.基于光谱分析技术的苹果花钾素含量估测研究[J].红外,2010,31(8):19-23.
• [24]TANG Yan-lin,WANG Xiu-zhen,HUANG Jing-feng,KONG Wei-shu,WANG Ren-chao.The Hyperspectra and their red edge characteristics of cotton(I)[J].Cotton Science,2003,15(3):l46-150.唐延林,王秀珍,黄敬峰,孔维姝,王人潮.棉花高光谱及其红边特征(I)[J].棉花学报,15(3):146-150.
• [25]LüQiang,HE Shao-lan,LIU Bin,TIAN Xi,YI Shi-lai,DENG Lie.Selection of NIR efficient regions for total soluble solids of Bamfild Navel Orange[J].Transactions of the Chinese Society for Agricultural Machinery,2012,43(z1):211-214.吕强,何绍兰,刘斌,田喜,易时来,邓烈.班菲尔脐橙可溶性固形物近红外光谱特征谱区选择[J].农业机械学报,2012,43(z1):211-214.
• [26]DELWICHE S R,BEAN M M,MILLER R E,WEBB B D,WILLIAMS P C.Apparent amylose content of milled rice by near-infrared reflectance spectrophotometry[J].Cereal Chemistry,1995,72:182-187.
• [27]ZHANG Hai-liang,LUI Xue-mei,HE Yong.Measurement of soil organic matter and available K based on SPA-LS-SVM[J].Spectroscopy and Spectral Analysis,2014,34(5):1348-1351.章海亮,刘雪梅,何勇.SPA-LA-SVM检测土壤有机质和速效钾研究[J].光谱与光谱学分析,2014,34(5):1348-1351.
• [28]WANG Jia-hua,LI Peng-fei,CAO Nan-ning,HAN Dong-hai.Study on the combination weight PLS model for determing SSC of peach based on the optimal information regions obtained from i PLS methods[J].Journal of Infrared and Millimeter Waves,2009,28(5):386-391.王加华,李鹏飞,曹楠宁,韩东海.基于i PLS原理最优化信息区间的桃糖度组合权重PLS模型研究[J].红外与毫米波学报,2009,28(5):386-391.