可可球二孢(Botryodiplodia theobromae)细胞壁降解酶对杧果果实致病作用Pathogenicity of cell wall degrading enzymes produced by Botryodiplodia theobromae against mango fruit
李敏;高兆银;胡美姣;周铄;杨冬平;杨波;易建学;杨凤珍;
摘要(Abstract)：
为明确可可球二孢(Botryodiplodia theobromae Pat.)产细胞壁降解酶在侵染杧果果实过程中的作用,研究了可可球二孢在离体培养和接种条件下分泌的5种细胞壁降解酶活性变化特点,以及可可球二孢(B.theobromae)、胶孢炭疽菌(Colletotrichum gloeosporioides Penz.)、杧果拟茎点霉(Phomopsis mangiferae Ahmad)和小穴壳菌(Dothiorella do-minicana Pet.et Cif.)4种杧果采后主要蒂腐病菌分泌细胞壁降解酶的能力。结果表明,可可球二孢在离体培养条件下或接种杧果果实后均可产生多聚半乳糖醛酸酶(PG)、果胶甲基半乳糖醛酸酶(PMG)、多聚半乳糖醛酸反式消除酶(PGTE)、果胶甲基反式消除酶(PMTE),以及纤维素酶(Cx)。其中PG、PMG和Cx活性较高,PGTE和PMTE活性极低。在大量分泌的3种细胞壁降解酶中,PG和Cx活性高峰出现较早,PMG活性高峰出现较迟。可可球二孢的致病力显著高于胶孢炭疽菌、杧果拟茎点霉和小穴壳菌。其可可球二孢分泌果胶酶的能力也显著高于其他3种病菌。
关键词(KeyWords)： 杧果;可可球二孢;细胞壁降解酶;致病力
基金项目(Foundation): 行业(农业)科研专项(3-44-2);; 中央级公益性科研院所基本科研业务费专项(N0.2009hzs1J011、NO.2011hzs1J027、NO.2011hzs1J004)
作者(Author): 李敏;高兆银;胡美姣;周铄;杨冬平;杨波;易建学;杨凤珍;
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