张佳卉;王力荣;曹珂;朱更瑞;方伟超;陈昌文;王蛟;

• 1:中国农业科学院郑州果树研究所

摘要(Abstract)：

果实糖、酸含量及其比例是评价桃果实品质的重要指标。主要综述桃果实糖、酸代谢关键酶和糖、酸QTLs的定位和候选基因研究进展。桃果实糖、酸代谢基本途径关键酶有蔗糖合成酶、蔗糖酸性转移酶、山梨醇氧化酶、山梨醇脱氢酶、NADP+-苹果酸酶、磷酸烯醇式丙酮酸羧化酶、柠檬酸合酶、磷酸烯醇式丙酮酸羧激酶等;通过遗传连锁图谱构建和连锁分析已定位到多个糖、酸组分QTLs,绝大多数位于第1、3、4、5、6、8连锁群;通过关键酶编码基因与QTLs共座位方法,分离到一系列候选基因。结合桃基因组测序数据,总结调控桃果实糖、酸性状的可能候选基因。针对目前桃糖、酸基因定位研究中存在的问题,展望实现桃果实糖、酸基因定位和单个基因克隆的新方法。

关键词(KeyWords)： 桃;糖;酸;QTLs

Abstract:

Keywords:

基金项目(Foundation): 十二五·863课题“桃分子育种与品质创新”(2011AA10020606);; 中央级公益性科研院所基本科研业务费专项(2012ZL046)

作者(Author): 张佳卉;王力荣;曹珂;朱更瑞;方伟超;陈昌文;王蛟;

Email:

参考文献(References)：

• [1]ZUO Qin-yuan,ZHU Geng-rui,WANG Li-rong.Several problems which should be paid attention during nectarine production development[J].Journal of Fruit Science,1996,13(3):206-207.左覃元,朱更瑞,王力荣.油桃生产发展中应注意的几个问题[J].果树科学,1996,13(3):206-207.
• [2]DIRLEWANGER E,MOING A,ROTHAN C,SVANELLA L,PRONIER V,GUYE A,PLOMION C,MONET R.Mapping QTLs controlling fruit quality in peach[Prunus persica(L.)Batsch][J].Theor Appl Genet,1999,98(1):18-31.
• [3]ETIENNE C,ROTHAN C,MOING A,PLOMION C,BODENES C,SVANELLA-DUMAS L,COSSON P,PRONIER V,MONET R,DIRLEWANGER E.Candidate genes and QTLs for sugar and or-ganic acid content in peach[Prunus persica(L.)Batsch][J].Theor Appl Genet,2002,105(1):145-159.
• [4]MARCO E,MARIA C M,FIORELLA S,ANTONINO N,LUIGI C,ENNIO L N,GIUSEPPE P.Quality evaluation of peaches and nec-tarines by electrochemical and multivariate analyses:relationship between analytical measurements and sensory attributes[J].Food Chemistry,1997,60(4):659-666.
• [5]PANGBOM R M.Relative taste intensitives of selected sugars and organic acids[J].J Food Sci,1963,28:726-733.
• [6]BASSI D,SELLE R.Evalution of fruit quality in peach and apricot[J].Adv Hort Sci,1990,4:107-112.
• [7]MOING A,SCANELLE L,ROLIN D,GAUDILLERE M,GAUDILLERE J P,MONET R.Compositional changes during the fruit development of two peach cultivars differing in juice acidity[J].J Am Soc Hortic Sci,2000,123:770-775.
• [8]WEBB K L,J W A BURLY.Sorbitol translocation in apple[J].Sci-ence,1962,137:766.
• [9]ZHOU Rui,SHU Huai-rui.Sorbitol and its metabolism in higher plants[J].Plant Physiology Communications,1993,29(5):384-390.周瑞,束怀瑞.高等植物中的山梨醇及其代谢[J].植物生理学通讯,1993,29(5):384-390.
• [10]BYME D H,NIKOLIC A N,BUMS E E.Variability in sugars,acids,firmness,and colour characteristics of12peach genotypes[J].J AmSoc Hort Sci,1991,116:1004-1006.
• [11]WU Ben-hong.Preliminany QTLs mapping for sugars and acids,and impact factors on sugars and acids in peach(P.persica(L.)Betsch)furit[D].Beijing:China Agricultural University,2003.吴本宏.桃糖酸品质的影响因素及糖酸分子标记的初步定位[D].北京:中国农业大学,2003.
• [12]WU Xiang-niu,DAI Yao-ren.Plant physiology[M].Beijing:Science Press,1982:149-151.吴相钮,戴尧仁译.植物生理学[M].北京:科学出版社1982:149-151.
• [13]COLARIC M,VEBERIC R,STAMPAR F,HUDINA M.Evaluation of peach and nectarine fruit quality and correlations between sensory and chemical attributes[J].J Sci Food Agric,2005,85:2611-2616.
• [14]NIU Jing,ZHAO Jian-bo,WU Ben-hong,LI Shao-hua,LIU Guo-jie,JAING Quan.Sugar and acid contents in peach and nectarine derived from different countries and species[J].Acta Horticulturae Sinica,2006,33(1):6-11.牛景,赵剑波,吴本宏,李绍华,刘国杰,姜全.不同来源桃种质果实糖酸组分含量特点的研究[J].园艺学报,2006,33(1):6-11.
• [15]DEJONG T M,GOUDRIAAN J.Modeling peach fruit growth and carbohydrate requirements:reevaluation of the double-sigmoid growth patten[J].J Am Soc Hortic Sci,1989,114(5):800-805.
• [16]BIANCO R L,RIEGER M,SUNG S S.Activities of sucrose and sor-bitol metabolizing enzymes in vegetative sinks of peach and correla-tion with sink growth rate[J].J Amer Soc Hort Sci,1999,124(4):381-388.
• [17]MORIGUCHI T,SANADA T,YAMAKI S.Seasonal fluctuation of some enzymes relating to sucrose and sorbitol metabolism in peach fruit[J].J Am Soc Hortic Sci,1990,115:278-281.
• [18]WU Guo-liang,CAI Hua-cheng,ZHANG Hong-ping,ZHU Chao,TIAN Jian-bao,ZHANG Yan,YANG Yan-jun.Changes of sugar content and related enzyme activities in the flesh of developing al-mond fruit[J].Journal of Fruit Science,2012,29(4):605-610.吴国良,蔡华成,张宏平,朱超,田建保,张燕,杨燕君.扁桃果实发育期果肉组织糖含量及相关酶活性的变化[J].果树学报,2012,29(4):605-610.
• [19]BIANCO R L,RIEGER M.Partitioning of sorbitol and sucrose catabolism within peach fruit[J].J Amer Soc Hort Sci,2002,127(1):115-122.
• [20]YAMADA X,NIWA N,SHIRATAKE K.YAMAKI S.cDNA cloning of NAD-dependent sorbitol dehydrogenase from peach fruit and its expression during fruit development[J].J Hortic Sci Biotechnol,2001,76:581-587.
• [21]WESTWOOD,M.N.Fruit growth and thinning[BOOK]
• [22]VERNICA A L,SONIA O,JULIA B,MARTIN A L,CLAUDIA A B,CLAUDIO O B,CARLOS S A,MARA V L,ALISDAIR R F,MARIA F D.Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each de-velopmental stage[J].Plant Physiology,2011,157:1696-1710.
• [23]WANG Li-rong.Heretable pleiotropic effects of glabrous and saucer shape mutant from peach[D].Tai’an:Doctor Dissertation of Shan-dong Agricultural University,2007.王力荣.桃果实无毛和扁平基因的遗传多效性研究[D].泰安:山东农业大学,2007.
• [24]WU Ben-hong,LI Shao-hua,QUILOT B,GENARD M,KERVEL-LA J.Influence of hairless of fruit epidermis and flesh color on con-tents of sugars and acids and their relationship in peach[J].Scientia Agricultura Sinica,2003,36(12):1540-1544.吴本宏,李绍华,Qoilot B,Genard M,Kervella J.桃果皮毛、果肉颜色对果实糖与酸含量的影响及相关性研究[J].中国农业科学,2003,36(12):1540-1544.
• [25]WEN Ien-chi,KOCH K E,SHERMAN W B.Comparing fruit and tree characteristics of two peaches and their nectarine mutants[J].J Amer Soc Hort Sci,1995,120(1):101-106.
• [26]CELIA M,CANTN,YOLANDA G,MARA A.Analysis of pheno-typic variation of sugar profile in different peach and nectarine[Prunus persica(L.)Batsch]breeding progenies[J].Journal of the Science of Food and Agriculture,2009,89(11):1909-1917.
• [27]GONZALEZ A R,MAUROMOUSTAKOS A,PROKAKIS G,ASE-LAGE J.Influence of year,cultivar and fruit maturity on quality of peach puree[J].Journal of Food Quality,1992,15(2):97-109.
• [28]MARINI R P,SOWERS D,MARINI M C.Peach fruit quality is af-fected by shade during final swell of fruit growth[J].J Am Soc Hortic Sci,1991,116:383-389.
• [29]KOBADHI K,GEMMA H,IWAHORI S.Abscisic acid content and sugar metabolism of peaches grown under water stress[J].J Am Soc Hortic Sci,2000,125:425-428.
• [30]CAI Cheng-lin,LI Shao-hua,XU Ying-chun.Water stress during and after the termination of the stress of the peach leaf carbohydrate metabolism[J].Plant Physiology Communications,2001,37(6):495-498.柴成林,李绍华,徐迎春.水分胁迫期间及胁迫解除后桃树叶片中的碳水化合物代谢[J].植物生理学通讯,2001,37(6):495-498.
• [31]ZHOU L,CHRISTOPHER DA,PAUL RE.Defoliation and fruit re-moval effects on papaya fruit production,sugar accumulation,and sucrose metabolism[J].Am Soc Hortic Sci,2000,125:644-652.
• [32]HAO Yan-yan,LI Miao-ling,ZHANG Hui-rong,NIU Tie-shuan.Influences on fruit quality under the micro environment of bagging and analysis about its mechanism[J].J Shanxi Agric Univ,2003,(3):238-242.郝燕燕,李妙玲,张惠荣,牛铁荃.套袋微环境对果实品质的影响及其机理分析[J].山西农业大学学报,2003(3):238-242.
• [33]JOOBEUR T,VIRUEL M A,VICENTE M C,JAUREGUI B,BALLESTER J,DETTORI M T,VERDE I,TRUCO M J,MESSEGUER R,BATLLE I,QUARTA R.DIRLEWANGER E,ARUS P.Construction of a saturated linkage map for Prunus using an almond×peach F2progeny[J].Theor Appl Genet,1998,97:1034-1041.
• [34]ARANZANA M J,PINEDA A,COSSON P A,DIRLEWANGER E,ASCASIBAR J,CIPRIANI G,RYDER C D,TESTOLIN R,ABBOTT A G,KING G J,IEZZONI A F,ARUS P.A set of simple-sequence repeat(SSR)markers covering the Prunusgenome[J].Theoretical and Applied Genetics,2003,106(5):819-825.
• [35]DIRLEWANGER E E,GRAZIANO T,JOOBEEU R F,GARRIGA-CALDERE,COSSONETAL P.Comparative mapping and marker assisted selection in Rosaceae fruit crops[J].Proc Natl Acad Sci US-A,2004,101:9891-9896.
• [36]JAMES W O,AUDREY M S,ANTONIO C,SUNETH S S,SUE H,GLORIA I,WANG D,CHEN C Y,ESTHER K,AMY F I.Construc-tion of an intra specific sweet cherry(Prunus avium L.)genetic linkage map and synteny analysis with the Prunusreference map[J].Tree Genetics Genomes,2008,4:897-910.
• [37]CHAPARRO J X,WERNER D J,O'MALLEY D,SEDEROFF R R.Targeted mapping and linkage analysis of morphological isozyme and RAPD markers in peach[J].Theor Appl Genet,1994,87:805-815.
• [38]DIRLEWANGER E,BODO C.Molecular genetic mapping of peach[J].Euphytica,1994,77:101-103.
• [39]RAJAPAKSE S,BETHOFF L E,HE G,ESTAGER A E,SCORZA R,VERDE I,BALLARD R E,BAIRD W V,CALLAHAN A,MONET R,ABBOTT A G.Genetic linkage mapping in peach using morphological,RFLP and RAPD markers[J].Theor Appl Genet,1995,90:503-510.
• [40]DIRLEWANGER E,PRONIER V,PARVERY R,ROTHAN C,GUYE A,MONET R.Genetic linkage map of peach[Prunus persica(L.)Batsch]using morphological and molecular markers[J].Theor Appl Genet,1998,97:888-895.
• [41]EBENZER A O,CAMERON P P,THOMAS M G,DAN E P,FREDRICK A B,CARLOS H C.A fruit quality gene map of Prunus[J].BMC Genomics,2009,10:587-599.
• [42]QIAO Fei,WANG Li-rong,FAN Chong-hui,ZHU Geng-rui,FANG Wei-chao.A general genetic linkage map for peach estab-lished by using RAPD and AFLP markers[J].Journal of Fruit Sci-ence,2006,23(5):766-769.乔飞,王力荣,范崇辉,朱更瑞,方伟超.利用AFLP和RAPD标记构建桃的遗传连锁图谱[J].果树学报,2006,23(5):766-769.
• [43]CAO Ke,WANG Li-rong,ZHU Geng-rui,FANG Wei-chao,CHEN Chang-wen.Genetic linkage maps constructing and markers linked with pistil development and double petal gene in peach[J].Ac-ta Horticulturae Sinica,2009,36(2):179-186.曹珂,王力荣,朱更瑞,方伟超,陈昌文.桃遗传图谱构建及两个花性状的分子标记[J].园艺学报,2009,36(2):179-186.
• [44]SHEN Zhi-jun,MA Rui-juan,YU Ming-liang,CAI Zhi-xiang,XU Jian-lan.Mapping QTLs controlling flavor quality of peach using a cross population between nectarine and flat nectarine[J].Acta Hor-ticulturae Sinica,2010,37(11):1735-1744.沈志军,马瑞娟,俞明亮,蔡志祥,许建兰.油蟠桃组合遗传连锁图谱构建及糖酸性状QTL分析[J].园艺学报,2010,37(11):1735-1744.
• [45]MARTNEZ-GARCA P J,PARFITT D E,OGUNDIWIN E A,FASS J,CHAN H M,AHMA R,LURIE S,DANDEKAR A,GRA-ZIEL T M,CRISOSTO C H.High density SNP mapping and QTL analysis for fruit quality characteristics in peach(Prunus persica L.)[J].Tree Genetic Genomic,2013,9(1):19-36.DOI:10.1007/s11295-012-0522-7.
• [46]ZHANG Li-yang,ZHANG Feng-lan,WANG Mei,LIU Xiu-cun,ZHAO You-yun,XUE Lin-bao.A linkagemap construction for Chi-nese cabbage based on AFLP,SSR,RAPD and isozyme markers using DH population[J].Acta Horticulturae Sinica,2005,32(2):249-255.张立阳,张凤兰,王美,刘秀村,赵岫云,薛林宝.大白菜永久高密度分子遗传图谱的构建[J].园艺学报,2005,32(2):249-255.
• [47]SI Jun,LI Cheng-qiong,REN Xue-song,SONG Hong-yuan,SONG Ming,WANG Xiao-jia.Construction of a molecular linkage map for clubroot resistance in cabbage using SSR markers(Brassica oleracea L.var.capitata)[J].Acta Phytopathologica Sinica,2011,41(3):305-309.司军,李成琼,任雪松,宋洪元,宋明,王小佳.结球甘蓝抗根肿病SSR分子连锁图谱的构建[J].植物病理学报,2011,41(3):305-309.
• [48]DIRLEWANGER E,COSSON P,RENAUD C,MONET R,MOING A,POESSEL J L.\.New detection of QTLs controlling major fruit quality components in peach[J].Acta Horticulturae,2006,713:65-72.
• [49]EDUARDO I,IGOR P,GIORGIANA C,DANIELE B,CARLO P,ALBERTO V,LAURA R.QTL analysis of fruit quality traits in two peach intraspecific populations and importance of maturity data pleiotropic effect[J].Tree Genetics Genomes,2011,7:323-335.
• [50]QUILOT B,WU B H,KERVELLA J,GENARD M,FOULONGNE M,MOREAU K.QTL analysis of quality traits in an advanced back-cross between Prunus persica cultivars and the wild relative species P.davidian[J].Theor Appl Gene,2004,109:884-897.
• [51]ETIENNE C,MORING A,DIRLEWANGER E,RAYMOND P,MONET R,ROTHAN C.Isolation and characterization of six peach cDNAs encoding key proteins in organic acid metabolism and solute accumulation:involvement in peach fruit acidity[J].Physiol Plant,2002,114:259-270.
• [52]YOSHIDA M.Genetical studies on the fruit quality of peach varieties[M].Bull Hort Res Sta A9Japon,1970:1-15.
• [53]MONET R.Genetic transmission of the“non-acid”character.Inci-dence on selection for quality[M]//In:InRA(ed),Eucarpia Sym-posium on Tree Fruit Breeding Angers,1979:273-276.
• [54]BOUDEHRI K,BENDAHMANE A,CARHINET G,TROADEC C,MOING A,DIRLEWANGER E.Phenotypic and fine genetic charac-terization of the D locus controlling fruit acidity in peach[J].BMC Plant Bio,2009,doi:10.1186/1471-2229-9-59.
• [55]DIRLEWANGER E,MOING A,PRONIER V,SVANELLA L,GUYE A,MONET R,ROTHAN C.Detection of QTLs controlling peach fruit acidity and sweetness[J].ISHS Acta Horticulturae465:IV International Peach Symposium,1997.
• [56]MNEJJA M,GARCIA-M J,AUDERGON JM,ARUS P.Prunus mi-crosatellite marker transferability across rosaceous crops[J].Tree Genet Genome,2010,6:689-700.
• [57]FAN C C,YU S B,WANG C G,XING Y Z.A causal C-A mutation in the second exon of GS3highly associated with rice grain length and validated as a functional marker[J].Theor Appl Genet,2009,118(3):465-472.
• [58]The International Peach Genome Initiative,VERDE I,Albert G AB-BOTT A G,SCALABRIN S.The high-quality draft genome of peach(Prunus-persica)identifies unique patterns of genetic diversity,do-mestication and genome evolution[J].Nature Genetics,2013,doi:10.1038/ng.2586.
• [59]ALBERT V S.Genetic Analysis:moving between linkage and associa-tion[J].Cold Spring Harb Protoc,2012,10:173-182.
• [60]PRICE A H.Believe it or not:QTLs are accurate[J].Trends Plant Sci,2006,11:213-216.
• [61]YANG Xiao-hong,YAN Jian-bing,ZHENG Yan-ping,YU Jian-ming,LI Jian-sheng.Reviews of association analysis for quantitative traits in plants[J].Acta Agronomica Sinica,2007,33(4):523-530.杨小红,严建兵,郑艳萍,余建明,李建生.植物数量性状关联分析研究进展[J].作物学报,2007,33(4):523-530.
• [62]HUANG X,WEI X,SANG T.Genome-wide association studies of14 agronomic traits in rice landraces[J].Nat Genet,2010,42(11):961-966.
• [63]KE C,LIRONG W,GENGRUI Z,WEICHAO F.Genetics diversity,linkage disequilibrium,and association mapping analyses of peach(Prunus persica)landrace in China[J].Tree Genetics Genomes,2012,8(5):975-990.