陈春丽,邓于洋,郭文武

• 1:华中农业大学园艺植物生物学教育部重点实验室

摘要(Abstract)：

【目的】为了改进以往人工方式观测染色体精准度的不足,【方法】采用MicroMeasure软件和Image-Pro Plus图像分析软件对4,6-联脒-2-苯基吲哚(DAPI)荧光染色的四季橘(Citrus madurensis Lour.Tseng)体细胞中期染色体分别进行核型分析和三维重建。【结果】得到四季橘的核型公式2n=18=16 m(2SAT)+2 sm,其核型类型为1B型,属于进化中较原始类型;获得四季橘染色体立体图,并依据每条前中期染色体(从短臂到长臂)DAPI荧光强度变化,构建出染色体带型模式图。【结论】该研究利用染色体图像分析软件对染色体进行识别,获得了较丰富的染色体核型数据信息,为柑橘细胞遗传学研究提供了新方法。

关键词(KeyWords)： 四季橘;图像分析;DAPI;核型

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31125024);; 国家863计划课题(2011AA100205);; 湖北省自然科学基金(2009CDB071)

作者(Author): 陈春丽,邓于洋,郭文武

DOI: 10.13925/j.cnki.gsxb.2012.04.031

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