刘萍;范七君;牛英;娄兵海;刘冰浩;邓崇岭;

• 1:广西柑橘生物学重点实验室·广西特色作物研究院

摘要(Abstract)：

【目的】探讨次氯酸钙在金柑果实贮藏保鲜上的应用效果。【方法】以‘金弹’(Fortunella crassifolia Swingle)为材料,分别使用质量浓度为0.15、0.6 g·L~(-1)的次氯酸钙水溶液浸泡金柑果实2 min(温度10~12℃),然后在室温为(10±2)℃、湿度为85%~90%的条件下贮藏30 d,每隔5~7 d取样1次,研究次氯酸钙处理对金柑果实腐烂率、品质以及抗氧化酶活性的影响。【结果】经0.6 g·L~(-1)次氯酸钙处理的果实总酸含量较低,在0.3%左右;贮藏第30天失重率达到4.2%,与对照相比提高1.4%;各处理间可溶性固形物含量在17%上下波动且差异不显著;次氯酸钙处理可有效降低果实腐烂率,减小果实青霉病病斑直径,提高贮藏过程中金柑果实过氧化物酶活性,减缓超氧化物歧化酶活性的降低速率,降低过氧化氢酶活性,减少过氧化氢含量,从而延缓果实衰老。【结论】次氯酸钙可有效延缓金柑果实衰老,在金柑贮藏保鲜中,不同采收期的金柑所需的次氯酸钙浓度不同,对于采收较晚的金柑,0.6 g·L~(-1)次氯酸钙浸泡2 min可有效降低果实腐烂率。

关键词(KeyWords)： 金柑;次氯酸钙;腐烂率;品质;抗氧化物酶

Abstract:

Keywords:

基金项目(Foundation): 广西柑橘生物学重点实验室项目(201301X007);; 广西自然科学基金(2014GXNSFBA118130;2013GXNSFBA019112;桂科合1347004-11);; 桂北柑橘综合试验站项目;优质变异单株“滑皮金柑”的砧木筛选及综合评价(2011GXNSFA018112)

作者(Author): 刘萍;范七君;牛英;娄兵海;刘冰浩;邓崇岭;

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DOI:

参考文献(References)：

• [1]JULIA B,SONJA W,PETER F,ROBER P.Efficacy of chlorine for inactivation of Escherichia coli on vegetables[J].Postharvest Biology and Technology,2000,19(2):187-192.
• [2]王玲利,刘超,黄艳花,李兴发,曹明.黄冠梨采后热处理和钙处理对其钙形态及细胞壁物质代谢的影响[J].园艺学报,2014,41(2):249-258.WANG Lingli,LI Chao,HUANG Yanhua,LI Xingfa,CAO Ming.Effects of postharvest heat and calcium treatments on calcium fractions and cell wall metabolism of huangguan pear fruit[J].Acta Horticulturae Sinica,2014,41(2):249-258.
• [3]梁庆沙,张东亚,李芳,冷平,胡建芳.钙处理对白凤桃果实冰温贮藏后细胞壁代谢物质的影响[J].果树学报,2009,26(5):714-718.LIANG Qingsha,ZHANG Dongya,LI Fang,LENG Ping,HU Jianfang.Effetcs of Ca treatment on metabolite of cell wall o f baifeng peach fruit stroed at ice point[J].Journal of Fruit Science,2009,26(5):714-718.
• [4]陈继群,刘丽贞,陈永忠,张海岚.不同钙处理对脐橙裂果及其细胞壁酶活性的影响[J].华南农业大学学报,2014,35(6):29-32.CHEN Jiqun,LIU Lizhen,CHEN Yongzhong,ZHANG Hailan.Effects of various calcium treatments on fruit cracking and cell wall enzyme activities in navel orange[J].Journal of South China Agricultural University,2014,35(6):29-32.
• [5]陈丽娟,王赵改,杨慧,张乐,王晓敏,史冠莹.真空与乳酸钙处理对双孢蘑菇采后贮藏品质的影响[J].中国农业科学,2015,49(14):2818-2826.CHEN Lijuan,WANG Zhaogai,YANG Hui,ZHANG Le,WANG Xiaomin,SHI Guanying.Effects of calcium salts based on vacuum infiltration on quality of postharvest Agaricus bisporus[J].Scientia Agricultura Sinica,2015,49(14):2818-2826.
• [6]宋虎卫,郑永华,袁卫明,张瑞越,杨立明.过氧乙酸结合钙处理对枇杷贮藏品质的影响[J].农业科学与技术,2013(10):1476-1481.SONG Huwei,ZHENG Yonghua,YUAN Weiming,ZHANG Ruiyue,YANG Liming,Effects of peracetic acid(PAA)combined with calcium treatments on storage quality of loquat fruit[J].Agricultural Science&Technology,2013(10):1476-1481.
• [7]李明娟,何新华,刘根华,李德安,周祥杰.采前喷钙结合低温处理对金柑果实贮藏品质的影响[J].西南农业学报,2012,25(2):630-634.LI Mingjuan,HE Xinhua,LIU Genhua,LI Dean,ZHOU Xiangjie.Effect of perharvest calcium sprays and low temperature treatment on storage quality of Fortunella crassifolia fruits[J].Southwest China Journal of Agricultural Sciences,2012,25(2):630-634.
• [8]王艳颖,刘程惠,田密霞,李婷婷,胡文忠.氯化钙处理对鲜切芹菜生理与品质的影响[J].食品安全质量检测学报,2015,6(7):2458-2463.WANG Yanying,LIU Chenghui,TIAN Mixia,LI Tingting,HU Wenzhong.Effects of calcium chloride treatment on the physiologic quality of fresh-cut celery[J].Journal of Food Safety and Quality,2015,6(7):2458-2463.
• [9]HERNANDEZ M P,ALMENAR E,DEL V V,VELEZ D,GAVARA R.Effect of chitosan coating aombined with postharvest calcium treatment on strawberry quality during refrigerated storage[J].Food Chemistry,2008,110(2):428-435.
• [10]曹永庆,曹艳平,李壮,任杰,冷平.采前喷钙对中华寿桃采后贮藏品质及褐变的影响[J].果树学报,2008,25(6):811-815.CAO Yongqing,CAO Yanping,LI Zhuang,REN Jie,LENG Ping.Effect of perharvest calcium sprays on quality attributes and flesh browning of Zhong hua shou tao peach fruit[J].Journal of Fruit Science,2008,25(6):811-815.
• [11]王玉玲,路贵龙,张新富,王然,杨绍兰.采前钙处理对黄金梨果顶硬化果实贮藏特性及Pp EXPA2基因表达的影响[J].现代食品科技,2016(2):1-11.WANG Yuling,LU Guilong,ZHANG Xinfu,WANG Ran,YANG Shaolan.Effects of perharvest calcium treatment on the storage characters of hard and whangkeumbae pear and the expressionanalysis of Pp EXPA2[J].Modern Foot Science and Technology,2016(2):1-11.
• [12]李金龙.采后不同浓度钙处理对贮藏期间李果实硬度及果胶含量的影响[J].中国林副特产,2015(2):20-22.LI Jinlong.Effect of various calcium concentrations dip treatment on firmness and pectin content during post-harvest of plum[J].Forest by-product and Speciality in China,2015(2):20-22.
• [13]徐凌,郝义,郝树池,王岩,吕任强.采前钙和钾处理对红灯樱桃果实采后生理的影响[J].果树学报,2009,26(4):568-571.XU Ling,HAO Yi,HAO Shuchi,WANG Yan,LüRenqiang.Effect of perharvest calcium and potassium treatments on post-harvest physiology of sweet cherry cv.Hongdeng[J].Journal of Fruit Science,2009,26(4):568-571.
• [14]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.LI Hesheng.Principles and technology of plant physiology and biochemistry experiments[M].Beijing:Higher Education Press,2000.
• [15]谭钺,王茂生,吕勐,崔海金.不同破眠处理对油桃休眠芽H2O2含量及相关酶活性的影响[J].中国农学通报,2014,30(28):128-132.TAN Yue,WANG Maosheng,LüMeng,CUI Haijin.Effects of different dormancy-bieaking treatemts on H2O2content and acticity of related enzymes of dormant nectarine buds[J].Chinese Agricultural Science Bulletin,2014,30(28):128-132.
• [16]牛锐敏,饶景萍,韩新花.不同采收期对红富士苹果贮藏品质的影响[J].西北农业学报,2006,15(3):171-174.NIU Ruimin,RAO Jingping,HAN Xinhua.Effect of harvest date on storage quality of fuji apple[J].Acta Agriculturae Boreali-Occidentalis Sinica,2006,15(3):171-174.
• [17]田世平,罗云波,王贵禧.园艺产品采后生物学基础[M].北京:科学出版社,2011.TIAN Shiping,LUO Yunbo,WANG Guixi.Biological basis of horticultural products[M].Beijing:The Science Publishing Company,2011.
• [18]ROGIERS S Y,KUMAR G N,KMOWLES N R.Maturation and ripening of fruit of Amelanchier alnifolia Nutt.are accompanied by increasing oxidative stress[J].Annals of Botany,1998,81:203-211.
• [19]生吉萍,代晓霞,刘灿,孙建军,申琳.H2O2在采后果蔬冷害中的作用机制[J].北京农学院学报,2007,22(3):77-80.SHENG Jiping,DAI Xiaoxia,LIU Can,SUN Jianjun,SHEN Lin.Mechanism of the effect of H2O2on chilling injury of post-harvest fruit and vegetable[J].Journal of Beijing University of Agriculture,2007,22(3):77-80.
• [20]韩晋,田世平.外源茉莉酸甲酯对黄瓜采后冷害及生理生化的影响[J].园艺学报,2006,33(2):289-293.HAN Jin,TIAN Shiping.Effects of exogenous methyi jasmonate on chilling injury and physiology and biochemistry in postharvest cucumber[J].Acta Horticulturae Sinica,2006,33(2):289-293.
• [21]郑小林,田世平,李博强,徐勇.外源草酸延缓采后芒果成熟及其生理基础的研究[J].中国农业科学,2007,40(8):1767-1773.ZHENG Xiaolin,TIAN Shiping,LI Boqiang,XU Yong.Physiological roles of exogenous oxalic acid in delaying ripening of mango fruit during storage[J].Scientia Agricultura Sincca,2007,40(8):1767-1773.
• [22]WANG Q,LAI T F,QIN G Z,TIAN S P.Response of jujube fruits to exogenous oxalic acid treatment based on proteomic analysis[J].Plant Cell Physiology,2009,50:230-242.
• [23]王炜,李鹏霞,黄开红,王毓宁,胡花丽.次氯酸钙处理对红富士苹果防御酶活性的影响[J].江西农业学报,2009,21(1):48-50.WANG Wei,LI Pengxia,HUANG Kaihong,WANG Minning,HU Huali.Effects of cakcium hypochlorite treatment on defense enzyme activity of stored red fuji apple[J].Acta Agriculturae Jiangxi,2009,21(1):48-50.
• [24]胡佳羽,刘亚敏,李鹏霞,王炜,尹克林.次氯酸钙对贮藏脐橙膜脂过氧化和保护酶活性的影响[J].食品科学,2009,30(14):292-295.HU Jiayu,LIU Yamin,LI Pengxia,WANG Wei,YIN Kelin.Effects of calcium hypochlorite on membrane lipid peroxidation and activity of defensive enzymes of“Newhall”Navel oranges(Citrus sinensis Osbeck Newhall)during storage[J].Food Science,2009,30(14):292-295.