柑橘全爪螨对4种杀螨剂的抗性监测及增效作用Resistance monitoring and synergism on four acaricides against Panonychus citri
刘永华;蒋红波;袁明龙;樊钰虎;杨丽红;陈静;王进军;
摘要(Abstract):
为探明近年来柑橘全爪螨田间种群对常用杀螨剂抗性的变化趋势,采用玻片浸渍法于2008—2009年连续2a监测了柑橘全爪螨重庆种群对甲氰菊酯、哒螨酮、阿维菌素和甲维盐4种常用杀螨剂的抗性,同时测定了磷酸三苯酯(TPP)、顺丁烯二酸二乙酯(DEM)和增效醚(PBO)的增效作用。结果表明,柑橘全爪螨对甲氰菊酯的抗性上升速度较快,抗性增长倍数达5.1倍,哒螨酮次之,抗性增长倍数为2.2倍。柑橘全爪螨对阿维菌素的抗性两年间变化很小,相反对甲维盐的敏感性有所升高。增效作用研究表明,TPP对甲氰菊酯、阿维菌素和甲维盐的增效倍数分别为3.2倍、2.0倍和3.8倍。DEM对哒螨酮和甲维盐的增效倍数分别为3.3倍和2.6倍。PBO对甲氰菊酯、阿维菌素、哒螨酮和甲维盐的增效倍数分别为2.7倍、1.7倍、2.4倍和7.2倍。而TPP对哒螨酮以及DEM对甲氰菊酯和阿维菌素均未表现出明显的增效作用。
关键词(KeyWords): 柑橘全爪螨;杀螨剂;增效剂;抗性
基金项目(Foundation): 公益性行业(农业)科研专项(nyhyzx07-057);; 重庆市杰出青年科学基金(CSTC,2009BA1042);; “十一五”科技支撑计划(2007BAD47B03);; 重庆市重大科技攻关计划(CSTC,2007AA1024)
作者(Author): 刘永华;蒋红波;袁明龙;樊钰虎;杨丽红;陈静;王进军;
Email:
DOI: 10.13925/j.cnki.gsxb.2010.04.018
参考文献(References):
- [1]TAKEYAMA K,MORI N,OSAKABE M.Effect of cytochrome P450inhibitor,piperonyl butoxide,on survival of Panonychus citri(Mc-Gregor)(Acari:Tetranychidae)on citrus leaves[J].Applied Entomol-ogy and Zoology,2006,41(3):487-491.
- [2]ASHIHARA W.Infestation and reproduction of the citrus red mite,Panonychus citri(McGregor)(Acari:Tetranychidae)on leguminous plants[J].Applied Entomology and Zoology,1987,22(4):512-518.
- [3]CHEN Da-rong,ZHONG Jie-ying,CHEN Xi-sheng.Monitoring on resistance of isocarbophos to Panonychus citri in Fuzhou[J].Plant Pro-tection,1990,16(3):12-13.陈达荣,钟捷英,陈细生.福州地区桔全爪螨对水胺硫磷抗性测定[J].植物保护,1990,16(3):12-13.
- [4]HUANG Han-jie,HUANG Yuan-yun.Monitoring on resistance of fenbutatin oxide to Panonychus citri in Guangdong province[J].Plant Protection,1994(1):17-18.黄汉杰,黄媛云.广东省桔全爪螨对托尔克的抗药性监测[J].植物保护,1994(1):17-18.
- [5]YU Xiao,XU Jia-li,FAN Qing-hai.A review of the resistance of spider mites(acari:Tetranychidae)to pesticides in China[J].Wu Yi Science Journal,2001,17:91-96.于晓,徐加利,范青海.中国叶螨抗药性研究进展[J].武夷科学,2001,17:91-96.
- [6]FURUHASHI K.Development of acaricide resistance in the citrus red mite,Panonychus citri(McGregor),in Shizuoka Prefecture[J].Proceedings of the Kanto-Tosan Plant Protection Society,1994,41:267-269.
- [7]MASUI S,OHISHI T,KASUYA K.Tatara present status of resis-tance of the citrus red mite,Panonychus citri(McGregor),to acari-cides in Shizuoka Prefecture[C].Proceedings of the Kanto-Tosan Plant Protection Society,1995,42:245-246.
- [8]CHEN Nian-chun,HUANG Hua-zhang,PENG Yun-liang.Moni-toring of pesticide-resistance and preliminary studies on main resis-tant mechanism of citric spider mite(Panonychus citri McGregor)[J].Pesticide,1996,35(9):15-19.陈年春,黄华章,彭云良.柑桔全爪螨抗药性监测及主导抗性机制的初步研究[J].农药,1996,35(9):15-19.
- [9]TANG Hui-lian,YI Zong-yun,LUO Cheng.Monitoring on resis-tance of dicofol and pyridaben to Panonychus citri[J].Pesticide,2000,39(11):42.唐会联,易宗云,罗成.桔全爪螨对三氯杀螨醇、哒螨灵的抗药性监测[J].农药,2000,39(11):42.
- [10]MENG He-sheng,WANG Kai-yun,JIANG Xing-yin,YI Mei-qin.Studies on the Resistance of Panonychus citri to Several Acaricides[J].Pesticide,2000,39(2):26-28.孟和生,王开运,姜兴印,仪美芹.桔全爪螨对常用杀螨剂的抗药性测定[J].农药,2000,39(2):26-28.
- [11]RAN C,CHEN Y,WANG JJ.Susceptibility and carboxylesterase activity of five field populations of Panonychus citri(McGregor)(Acari:Tetranychidae)to four acaricides[J].International Journal of Acarology,2009,35(2):115-121.
- [12]BUSVINE J R.Recommended methods for measurement of resistance to pesticides[M].FAO Plant Production and Protection Paper,1980,21:49-54.
- [13]MENG He-sheng,WANG Kai-yun,JIANG Xing-yin,YI Mei-qin.Selection and biochemical mechanism of the resistance to pyridaben in Panonychus citri[J].Chinese Journal of Pesticide Science,2000,2(3):30-34.孟和生,王开运,姜兴印,仪美芹.桔全爪螨对哒螨灵抗性的选育及其生化机理[J].农药学学报,2000,2(3):30-34.
- [14]PAN Zhi-ping,LI Dun-song.Research advances in monitoring and detecting insect pesticide-resistance[J].Chinese Journal of Applied Ecology,2006,17(8):1539-1543.潘志萍,李敦松.昆虫抗药性监测与检测技术研究进展[J].应用生态学报,2006,17(8):1539-1543.
- [15]MENG He-sheng,WANG Kai-yun,JIANG Xing-yin,YI Mei-qin.Studies on the resistance selection of Panonychus citri to fen-propathrin and its cross resistance[J].Plant Protection,2000,26(6):7-9.孟和生,王开运,姜兴印,仪美芹.桔全爪螨对甲氰菊酯的抗性选育及交互抗性的研究[J].植物保护,2000,26(6):7-9.
- [16]KIM Y J,LEE S W,CHO J R,PARK H M,AHN Y J.Multiple re-sistance and biochemical mechanisms of dicofol resistance in Te-tranychus urticae(Acari:Tetranychidae)[J].Journal of Asia-Pacific Entomology,2007,10(2):165-170.
- [17]RAN Chun,CHEN Yang,YUAN Ming-long,WANG Jin-jun,LIU Hong-qiang,YAO Ting-shan.Susceptibility of Panonychus citri field populations to different acaricides[J].Acta Phytophylagica Sinica,2008,35(6):537-540.冉春,陈洋,袁明龙,王进军,刘浩强,姚廷山.桔全爪螨田间种群对杀螨剂的敏感性[J].植物保护学报,2008,35(6):537-540.
- [18]YAMAMOTO A,YONEDA H,HATANO R,ASADA M.Field se-lection experiments with hexythiazox in the citrus red mite,Panony-chus citri(McGregor)[J].Journal of Pesticide Science,1995,20(3):521-527.
- [19]CHEN Yang,RAN Chun,XIONG Lin,WANG Jin-jun.Influence of citruses germplasm on the carboxylesterase activity and acaricide susceptibility of Panonychus citri McGregor[J].Southwest China Jour-nal of Agricultural Sciences,2006,19(3):434-437.陈洋,冉春,熊琳,王进军.不同柑桔种质资源对桔全爪螨酶活性及其对药剂敏感性的影响[J].西南农业学报,2006,19(3):434-437.
- [20]YUAN Ming-long,RAN Chun,LI Yong,WANG Jin-jun.Effect of different citrus germplasms on acaricide susceptibility and esterase isozymes of Panonychus citri McGregor[J].Acta Phytophylacica Sini-ca,2008,35(2):187-188.袁明龙,冉春,李勇,王进军.取食不同柑桔种质资源对桔全爪螨药剂敏感性及酯酶同工酶的影响[J].植物保护学报,2008,35(2):187-188.
- [21]HE Lin,ZHAO Zhi-mo,DENG Xin-ping,WANG Jin-jun,LIU Huai,LIU Ying-hong.Selection and risk assessment of resistance to fenpropathrin,abamectin and pyridaben in Tetranychus cinnabari-nus[J].Acta Entomologica Sinica,2002,45(5):688-692.何林,赵志模,邓新平,王进军,刘怀,刘映红.朱砂叶螨对三种杀螨剂的抗性选育与抗性风险评估[J].昆虫学报,2002,45(5):688-692.
- [22]AHMAD M,SAYYED A H,SALEEM M A,AHMAD M.Evidence for field evolved resistance to newer insecticides in Spodoptera litura(Lepidoptera:Noctuidae)from Pakistan[J].Crop Protection,2008,27(10):1367-1372.
- [23]PENG Mei,SU Wen-wei,DENG Xin-ping.Synergism of3syner-gists to some insecticides and their inhibiting effects on enzymes[J].Journal of Southwest Agricultural University:Natural Science Edi-tion,2006,28(3):475-477,482.彭梅,苏文伟,邓新平.3种增效剂对药剂的增效作用及酶的抑制作用[J].西南农业大学学报:自然科学版,2006,28(3):475-477,482.
- [24]HE L,XUE C H,WANG J J,LI M,LU W C,ZHAO Z Z.Resis-tance selection and biochemical mechanism of resistance to two Aca-ricides in Tetranychus cinnabarinus(Boiduval)[J].Pesticide Bio-chemistry and Physiology,2009,93(1):47-52.