槜李等15个李品种S基因型鉴定及其多态性分析Identification of S-genotypes of Japanese plum cultivars by PCR and analysis on polymorphism of S-RNase genes
张树军;黄绍西;张绍铃;吴俊;衡伟;吴华清;宋宏峰;
摘要(Abstract):
利用李属S-RNase基因特异性引物,对15个供试李品种进行PCR扩增,共获得30个目的条带。对这些目的条带进行测序鉴定出15个李品种的S基因型。通过与NCBI中利用BLASTn与GenBank+EMBL+DDBJ+PDB等数据库中的序列比对,结果表明,其中9个为新S-RNases基因,对9个新S-RNases核苷酸序列进行分析发现,位于高变区内的内含子大小为141~1758bp,其同源性为33.9%(S-18~S-19)~81.6%(S-20~S-21),表现出丰富的长度和序列多态性;编码区的核苷酸序列比对结果,其同源性为73.3%(S-16~S-19)~91.7%(S-17~S-22);其推导氨基酸序列相似性为67.3%(S-16~S-19)~89.1%(S-17~S-22);包含李属S-RNase一级结构所共有的C2、C3保守区和高变区(RHV)。系统进化分析表明,9个新S-RNases与李属其它树种S-RNases聚类在一起,归属为李亚科(Prunoideae)。
关键词(KeyWords): 李;自交不亲和性;S-RNase基因
基金项目(Foundation): 国家自然科学基金(30671437)
作者(Author): 张树军;黄绍西;张绍铃;吴俊;衡伟;吴华清;宋宏峰;
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DOI:
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