张树军;黄绍西;张绍铃;吴俊;衡伟;吴华清;宋宏峰;

• 1:南京农业大学园艺学院
• 2:南京农业大学园艺学院
• 3:南京农业大学园艺学院
• 4:南京农业大学园艺学院

摘要(Abstract)：

利用李属S-RNase基因特异性引物,对15个供试李品种进行PCR扩增,共获得30个目的条带。对这些目的条带进行测序鉴定出15个李品种的S基因型。通过与NCBI中利用BLASTn与GenBank+EMBL+DDBJ+PDB等数据库中的序列比对,结果表明,其中9个为新S-RNases基因,对9个新S-RNases核苷酸序列进行分析发现,位于高变区内的内含子大小为141～1758bp,其同源性为33.9%(S-18～S-19)～81.6%(S-20～S-21),表现出丰富的长度和序列多态性;编码区的核苷酸序列比对结果,其同源性为73.3%(S-16～S-19)～91.7%(S-17～S-22);其推导氨基酸序列相似性为67.3%(S-16～S-19)～89.1%(S-17～S-22);包含李属S-RNase一级结构所共有的C2、C3保守区和高变区(RHV)。系统进化分析表明,9个新S-RNases与李属其它树种S-RNases聚类在一起,归属为李亚科(Prunoideae)。

关键词(KeyWords)： 李;自交不亲和性;S-RNase基因

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30671437)

作者(Author): 张树军;黄绍西;张绍铃;吴俊;衡伟;吴华清;宋宏峰;

Email:

DOI:

参考文献(References)：

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