齐希梁;李明;刘聪利;宋露露;

• 1:中国农业科学院郑州果树研究所

摘要(Abstract)：

【目的】建立欧洲甜樱桃果实VIGS体系。【方法】利用In-Fusion cloning技术,构建欧洲甜樱桃的烟草脆裂病毒(TRV)介导基因沉默表达载体pTRV2-ANS,转入农杆菌GV3101中,采用注射压迫法侵染欧洲甜樱桃果实,通过分子检测、qRT-PCR和表型观察技术评价TRV介导VIGS沉默体系的效果。【结果】TRV::ANS侵染的欧洲甜樱桃果实ans基因的表达显著低于TRV::00侵染的果实。与TRV::00比较,TRV::ANS侵染的欧洲甜樱桃果实的果皮和果肉颜色都没有发生着色,果皮和果肉颜色呈绿色,而TRV::00侵染的欧洲甜樱桃果实的果皮和果肉正常着色。【结论】研究建立的TRV介导欧洲甜樱桃VIGS体系可有效沉默欧洲甜樱桃果实内源基因表达。该体系为欧洲甜樱桃果实品质基因的功能验证及分子机制研究奠定了技术基础。

关键词(KeyWords)： 欧洲甜樱桃;TRV;VIGS;基因沉默;果实

Abstract:

Keywords:

基金项目(Foundation): 中国农业科学院科技创新工程专项(CAAS-ASTIP-2018-ZFRI);; 中央级科研院所基本科研业务费专项(1610192018201)

作者(Author): 齐希梁;李明;刘聪利;宋露露;

Email:

DOI: 10.13925/j.cnki.gsxb.20180188

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