草莓果实蔗糖和己糖的代谢特性及其与糖积累的关系(英文)Characteristics of sucrose and hexose metabolism in relation to sugar accumulation in developing strawberry fruit
陈俊伟;秦巧平;谢鸣;蒋桂华;徐红霞;程建徽;吴江;孙崇波;
摘要(Abstract):
以设施栽培的枥乙女草莓为试材,研究蔗糖代谢和己糖代谢关键酶在草莓果实糖积累进程中的作用。采集花后1~8周的果实,分别测定果实中葡萄糖、果糖、蔗糖的含量和蔗糖磷酸合酶(SPS)、蔗糖合酶(SS)、可溶性酸性转化酶(AI)、中性转化酶(NI)、己糖激酶(HXK)及果糖激酶(FRK)等蔗糖和己糖代谢酶的活性。结果表明,草莓果实的总糖含量随果实发育持续积累,但蔗糖的快速积累对果实后期糖的积累贡献最大。在果实发育前期,草莓AI活性随果实发育而下降,但在果实发育后期,AI活力随果实成熟而急剧上升,这使果实最终以积累己糖为主。果实蔗糖快速积累期,合成蔗糖的酶SPS和SS(合成方向)的活性下降到较低水平,表明这2种酶对草莓后期果实蔗糖的快速积累贡献较少。果实发育后期较低的HXK和FRK活力有利于果实糖的快速积累。
关键词(KeyWords): 草莓;蔗糖代谢;己糖代谢;糖积累
基金项目(Foundation): 国家自然科学基金(30370998);; 浙江省自然科学基金(302391)。
作者(Author): 陈俊伟;秦巧平;谢鸣;蒋桂华;徐红霞;程建徽;吴江;孙崇波;
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