高宁宁;李晓慧;康利允;常高正;梁慎;徐小利;李海伦;王慧颖;赵卫星;

• 1:河南省农业科学院园艺研究所

摘要(Abstract)：

【目的】对厚皮甜瓜未受精子房诱导单倍体形成的影响因素进行研究,以期为植物遗传理论研究及实际育种提供新材料和技术支持。【方法】以3个厚皮甜瓜品种为试材,对甜瓜未受精子房进行离体培养,比较不同基因型、不同栽培季节、不同热激时间、不同的接种方式和不同激素对胚状体诱导的影响,以及不同激素对胚状体成苗的影响。【结果】3种不同基因型甜瓜中,‘将军玉’的胚状体发生频率最高,为38.3%,夏播材料的诱导频率高于春播材料,35℃黑暗热激处理接种材料3 d出胚率最高,子房横切或纵切对胚状体诱导影响不大,MS培养基在含0.03 mg·L~(-1)TDZ基础上添加0.2 mg·L~(-1)KT、0.01 mg·L~(-1)IAA和15%椰汁出胚率最高,为46.7%,在添加0.2 mg·L~(-1)KT的MS培养基上,胚芽伸长较快。研究所获得的再生植株群体较为复杂,包括单倍体、二倍体、三倍体和混倍体等多种类型。【结论】不同基因型甜瓜材料和不同栽培季节对胚状体的诱导率有很大影响,35℃黑暗热激处理离体子房3 d出胚效果最好,最佳的胚状体诱导培养基为MS+0.03 mg·L~(-1)TDZ+0.2 mg·L~(-1)KT+0.01 mg·L~(-1)IAA+15%椰汁。

关键词(KeyWords)： 甜瓜;未受精子房;离体培养;单倍体

Abstract:

Keywords:

基金项目(Foundation): 国家西甜瓜产业技术体系建设（CARS-25）;; 河南省农业科学院优秀青年科技基金（2018YQ12）;河南省农业科学院自主创新

作者(Author): 高宁宁;李晓慧;康利允;常高正;梁慎;徐小利;李海伦;王慧颖;赵卫星;

Email:

DOI: 10.13925/j.cnki.gsxb.20200117

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