曹珂;王力荣;朱更瑞;方伟超;陈昌文;赵佩;

• 1:中国农业科学院郑州果树研究所

摘要(Abstract)：

桃果肉颜色是与果品加工密切相关的一种重要经济性状。以桃栽培种瑞光19(白肉)×Summergrand(黄肉)杂交的104株F1后代分离群体为试材,对桃白肉/黄肉的SSR分子标记进行研究,以期为该性状的分子标记辅助育种奠定理论基础。结果表明,筛选的135对引物中有4对引物,CPDCT024、pchgms3、CPPCT026和BPPCT011,在白肉和黄肉单株以及混合基因池间扩增出差异。分析性状与标记的连锁情况表明,CPDCT024与白/黄肉性状遗传距离最近,为7.8cM。试验构建了桃白/黄肉性状的连锁群,根据4个SSR标记与参考图谱T×E和P2175×GN比对,将控制白/黄肉性状的基因定位在参考图谱第1连锁群,对应于桃的第1条染色体。以CPDCT024和参考图谱上另一个与白/黄肉性状连锁距离较近的标记CPPCT026在20个白肉和黄肉品种上验证,CPDCT024检测的准确率为70%,优于CPPCT026。

关键词(KeyWords)： 桃;果肉颜色;SSR;分子标记

Abstract:

Keywords:

基金项目(Foundation): 国家863项目(2006AA100108-4-12);; 国家桃产业技术体系(nycytx-31-1-2)

作者(Author): 曹珂;王力荣;朱更瑞;方伟超;陈昌文;赵佩;

Email:

DOI: 10.13925/j.cnki.gsxb.2010.06.025

参考文献(References)：

• [1]BAILEY J S,FRENCH A P.The inheritance of certain fruit and fo-liage characters in the peach[J].Mass Agric Expt Stn Bull,1949,452,2-31.
• [2]WANG Zu-hua,ZHUANG En-ji.Fruit monograph for peach in China[M].Beijing:China Forest Press,2001.汪祖华,庄恩及.中国果树志·桃卷[M].北京:中国林业出版社,2001.
• [3]WARBURTON M L,BECERRA V L,GOFFREDA J C,BLISS F A.Utility of RAPD markers in indentifying genetic linkages to genes of economic interest in peach[J].Theor Appl Genet,1996,93:920-925.
• [4]SOSINSKI B,SOSSEY A K,LU Z X,RAJAPAKSE S,GLASS-MOYER K,BALLARD R E,ABBOTT A G,BAIRD W V,REIGHARD G,TABB A,SCORZA R.Use of AFLP and RFLP markers to create a combined linkage map in peach[Prunus peasica(L.)Batsch]for use in marker assisted selection[J].Acta Horticulture,1998,465:61-89.
• [5]ABBOTT A G,RAJAPAKSE S,SOSINSKI B.Construction of satu-rated linkage maps of peach crosses segregating for characters con-trolling fruit quality,tree architecture and pest resistance[J].Acta Horticulturae,1998,465:141-150.
• [6]YANG Ying-jun,ZHANG Kai-chun,LI Rong-qi,LIN Ke,YIN Shu-ping.JIANG Quan,ZHANG Xiao-ming.RAPD markers linked to nectarine gene and flesh color gene of peach fruit[J].Acta Agri-culturae Boreali-Sinica,2000,15(3):6-9.杨英军,张开春,李荣旗,林珂,尹淑萍,姜全,张晓明.桃果实有毛/无毛、白肉/黄肉性状的RAPD分子标记[J].华北农学报,2000,15(3):6-9.
• [7]GAO Hui-min.Translating of F gene and Y gene of Peaeh(Prunus persica(L.)Batseh)to SCAR markers[D].Baoding:Hebei agriculture university,2002.高慧敏.桃(Prunus persica(L.)Batseh)白肉基因(Y)和离核基因(F)的SCAR标记转化[D].保定:河北农业大学硕士论文,2002.
• [8]BLISS F A,ARULSEKAR S,FOOLAD M R.An expanded genetic linkage map of Prunus based on an interspecific cross between al-mond and peach[J].Genome,2002,45:520-529.
• [9]ELISABETH D,ENRIQUE G,TAREK J,FRANCESC G C,PATRICK C,WERNER H,PERE A.Comparative mapping and marker-assisted selection in Rosaceae fruit crops[J].PNAS,2004,101(26):9891-9896.
• [10]WANG Zhi-gang,HAN Ming-yu,ZHAO Cai-ping,ZHANG Gui-fen.RAPD Markers of flesh color of nectarine[J].Acta Bot Boreal.-Occident Sin,2006,26(2):0300-0304.王志刚,韩明玉,赵彩平,张桂粉.油桃果肉颜色性状的RAPD分子标记研究[J].西北植物学报,2006,26(2):0300-0304.
• [11]YU Ming-liang,MA Rui-juan,SHEN Zhi-jun,ZHANG Zhen.Molecular markers linked to specific characteristics of Prunus persi-ca(L.)Batsch.[J].Acta Horticulturae Sinica,2006,33(3):511-517.俞明亮,马瑞娟,沈志军,章镇.桃果肉颜色、果皮茸毛和花粉育性性状的分子标记[J].园艺学报,2006,33(3):511-517.
• [12]GAO Yan,HAN Ming-yu,ZHAO Cai-ping,SONG Jian.Construc-tion of AFLP molecular markers linkage map in nectarine[J].Journal of Fruit Science,2008,25(4):478-484.高妍,韩明玉,赵彩平,宋健.桃分子连锁图谱的构建[J].果树学报,2008,25(4):478-484.
• [13]SONG Jian,GAO Yan,HAN Ming-yu,ZHAO Cai-ping.SSR Markers linked to flesh color and flesh adhesion characteristics of Peach[J].Acta Agriculturae Boreali occidentalis Sinica,2008,17(4):234-237.宋健,高妍,韩明玉,赵彩平.桃果肉颜色、离粘核性状的SSR标记[J].西北农业学报,2008,17(4):234-237.
• [14]CAO Ke,WANG Li-rong,ZHU Geng-rui,FANG Wei-chao,CHEN Chang-wen.Genetic linkage maps monstructing and markers linked with pistil development and double petal gene in Peach[J].Acta Horticulturae Sinica,2009,36(2):179-186.曹珂,王力荣,朱更瑞,方伟超,陈昌文.桃遗传图谱的构建及两个花性状的分子标记[J].园艺学报,2009,36(2):179-186.
• [15]MNEJJA M,GARCIA M J,HOWAD W,ARUS P.Development and transportability across Prunus species of42polymorphic almond mi-crosatellites[J].Molecular ecology notes,2005,5(3):531-535.
• [16]ARANZANA M J,GARCIA M J,CARBO J,ARUS P.Development and variability analysis of microsatellite markers in peach[J].Plant breed,2002,121(1):87-92.
• [17]YUN Bi-xu,JONATHAN H C.Marker-assisted selection in plant breeding:from publications to practice[J].Crop Science,2008,48:391-407.