罗静,张巧莲,武峥,漆巨容,谢永红,王武,王进,刘正富

• 1:中国农业科学院郑州果树研究所
• 2:重庆农业科学院果树研究所
• 3:许昌职业技术学院园艺园林系

摘要(Abstract)：

【目的】桃在冬天为了避免低温伤害而停止生长的发育过程称之为自然休眠。为研究桃花芽休眠解除的分子遗传机制,筛选休眠相关基因。【方法】以中需冷量桃品种‘满天红’为试材,采用cDNA-AFLP技术鉴定花芽内差异表达的基因,并对上调基因进行功能预测。【结果】12对引物共扩增出525条差异TDFs(transcripts-derived fragment),其中与休眠解除相关的上调TDFs 14条,下调的TDFs 18条。对休眠解除后上调的14个差异条带进行克隆测序,经Blast显示其中10个基因分别与泛素连接酶E3、植物组蛋白H3、核糖体蛋白L34、Prohibitin-2蛋白以及半乳糖基转移酶等基因同源性较高。【结论】筛选到的休眠相关基因功能涉及代谢、逆境响应、细胞分化及信号转导,这些基因有助于我们更好的解析桃花芽休眠的机制。

关键词(KeyWords)： 桃;休眠;差异表达基因;cDNA-AFLP

Abstract:

Keywords:

基金项目(Foundation): 重庆市自然科学基金计划项目(CSTC,2009BB1247)

作者(Author): 罗静,张巧莲,武峥,漆巨容,谢永红,王武,王进,刘正富

DOI: 10.13925/j.cnki.gsxb.2012.04.014

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