董雅凤;张尊平;刘凤之;王宝亮;张少瑜;

• 1:中国农业科学院果树研究所
• 2:中国农业科学院果树研究所
• 3:中国农业科学院果树研究所
• 4:中国农业科学院果树研究所

摘要(Abstract)：

为建立快速、灵敏、可靠的沙地葡萄茎痘相关病毒(GRSPaV)检测方法,以总RNA为模板,采用2组GRSPaV特异性引物对29个品种52株葡萄样品进行RT-PCR检测,并对扩增产物进行了测序和分析。结果表明,从20个品种25株葡萄样品中检测到GRSPaV,平均带毒株率为48.1%。外壳蛋白基因片段引物F1/R1从25个样品中扩增到905bp的特异片段,复制酶基因片段引物F2/R2从20个样品中扩增到498bp的特异片段,表明GRSPaV外壳蛋白基因比复制酶基因更加保守,RT-PCR检测时采用F1/R1则更为适宜。PCR产物测序结果与GenBank中登录的GRSPaV序列比较,同源性为97.90%～98.11%。

关键词(KeyWords)： 沙地葡萄茎痘相关病毒;RT-PCR;检测

Abstract:

Keywords:

基金项目(Foundation): 辽宁省自然科学基金(20032139);; 人事部留学回国人员科技活动择优资助项目(农办人[2004]121号);; 辽宁省葫芦岛市科技攻关项目(04105B1601)

作者(Author): 董雅凤;张尊平;刘凤之;王宝亮;张少瑜;

Email:

参考文献(References)：

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