不同猕猴桃品种果实发育过程中总酚和类黄酮含量及抗氧化活性的动态变化Dynamic changes in total phenols, flavonoids and antioxidant capacity during fruit development of different kiwifruit cultivars
黄春辉;廖光联;谢敏;陶俊杰;曲雪艳;陈璐;徐小彪;
摘要(Abstract):
【目的】探讨不同猕猴桃品种果实发育过程中总酚、类黄酮含量及抗氧化活性的动态变化差异。【方法】以江西主栽的美味猕猴桃'金魁’和中华猕猴桃'红阳’以及本课题组自主选育的毛花猕猴桃'赣猕6号’为试材,对果实生长发育过程中总酚、类黄酮含量和FRAP抗氧化能力、清除羟基自由基(·OH)、DPPH自由基(DPPH·)和超氧阴离子自由基(·O2-)的能力进行检测分析。【结果】3个猕猴桃品种果实总酚和类黄酮含量在果实生长发育过程中大体呈现由高到低的变化趋势;但不同品种其含量差异显著,尤其'赣猕6号’总酚含量高于其他两个品种十几倍。对抗氧化活性检测发现',赣猕6号’比其他两个品种具有较强的FRAP抗氧化能力和清除超氧阴离子自由基(·O2-)能力。相关性分析显示',赣猕6号’清除·OH和·O2-能力与总酚、类黄酮含量存在极显著或显著相关性',金魁’FRAP抗氧化能力与总酚、类黄酮含量存在极显著和显著相关性,而'红阳’FRAP抗氧化能力、清除DPPH·能力分别与总酚、类黄酮含量存在显著和极显著相关性。【结论】猕猴桃果实含有丰富的酚类物质,随着果实的生长发育呈现逐渐降低的趋势。猕猴桃抗氧化能力与总酚、类黄酮含量呈显著正相关。毛花猕猴桃'赣猕6号’比美味猕猴桃'金魁’和中华猕猴桃'红阳’具有更高含量的酚类物质和更强的抗氧化能力。本试验为猕猴桃优良品种的推广、野生资源发掘、新品种选育、天然抗氧化保健药物以及功能性食品的开发奠定了基础。
关键词(KeyWords): 猕猴桃;果实发育;总酚;类黄酮;抗氧化能力
基金项目(Foundation): 国家自然科学基金(31760559;31760567;31460505);; 江西省现代农业产业技术体系建设专项(JXARS-05)
作者(Author): 黄春辉;廖光联;谢敏;陶俊杰;曲雪艳;陈璐;徐小彪;
Email:
DOI: 10.13925/j.cnki.gsxb.20180115
参考文献(References):
- [1]钟彩虹,张鹏,姜正旺,王圣梅,韩飞,徐丽云,黄宏文.中华猕猴桃和毛花猕猴桃果实碳水化合物及维生素C的动态变化研究[J].植物科学学报,2011,29(3):370-376.ZHONG Caihong,ZHANG Peng,JIANG Zhengwang,WANGShengmei,HAN Fei,XU Liyun,HUANG Hongwen. Dynmicchanges of carbogydrate and vitamin C in fruits of Actinidia chi-nensis and A. eriantha during growing season[J]. Plant ScienceJournal,2011,29(3):370-376.
- [2]汤佳乐,黄春辉,刘科鹏,高洁,吴寒,徐小彪.野生毛花猕猴桃叶片与果实AsA含量变异分析[J].江西农业大学学报,2013,35(5):982-987.TANG Jiale,HUANG Chunhui,LIU Kepeng,GAO Jie,WUHan,XU Xiaobiao. Variation analysis of AsA content in leavesand fruits of wild Actinidia eriantha[J]. Journal of Jiangxi Agri-cultural University,2013,35(5):982-987.
- [3] ARTI B,RAKESH M,PUSHPA L,GURU S K,ARORA S.Role of secondary metabolites and brassinosteroids in plant de-fense against environmental stresses[J]. Journal of Plant GrowthRegulation,2013,32(1):216-232.
- [4] LATTANZIO V,LATTANZIO V M T,CARDINALI A. Role ofphenolics in the resistance mechanisms of plants against fungalpathogens and insects[J]. Phytochemistry Advances in Research,2006,37(4):23-27.
- [5] SCALBERT A,MANACH C,MORAND C. Dietary polyphe-nols and the prevention of diseases[J]. Critical Reviews in FoodScience&Nutrition,2005,45(4):287-306.
- [6] DAI J,MUMPER R J. Plant phenolics:Extraction,analysis andtheir antioxidant and anticancer properties[J]. Molecules,2010,15(10):7313-7352.
- [7] SOTO-VACA A,GUTIERREZ A,LOSSO J N,XU Z M,FinleyJ W. Evolution of phenolic compounds from color and flavorproblems to health benefits[J]. Journal of Agricultural and FoodChemistry,2012,60(27):6658-6677.
- [8]严娟,蔡志翔,沈志军,张斌斌,钱巍,俞明亮.桃3种颜色果肉中10种酚类物质的测定及比较[J].园艺学报,2014,41(2):319-328.YAN Juan,CAI Zhixiang,SHEN Zhijun,ZHANG Binbin,QIAN Wei,YU Mingliang. Determination and comparison of 10phenolic compounds in peach with three types of flesh color[J].Acta Horticulturae Sinica,2014,41(2):319-328.
- [9] TOMáS-BARBERáN F A,ESPíN J C. Phenolic compoundsand related enzymes as determinants of quality in fruits and veg-etables[J]. Journal of Science Food Agriculture,2001,81:853-876.
- [10] OGAH O,WATKINS S C,UBI E B,ORGUZIE N C. Phenoliccompounds in rosaceae fruit and nut crops[J]. Journal of Agricul-tural and Food Chemistry,2014,62(39):9369-9386.
- [11]仇占南,张茹阳,彭明朗,张文,李天忠,朱元娣.北京野生软枣猕猴桃果实品质综合评价体系[J].中国农业大学学报,2017,22(2):45-53.QIU Zhannan,ZHANG Ruyang,PENG Minglang,ZHANGWen,LI Tianzhong,ZHU Yuandi. Comprehensive evaluationsystem of the fruit quality of wild Actinidia arguta in Beijing[J].Journal of China Agricultural University,2017,22(2):45-53.
- [12] COOK N C,SAMMAN S. Flavonoids-Chemistry,metabolism,cardioprotective effects,and dietary sources[J]. Journal of Nutri-tional Biochemistry,1996,7(2):66-76.
- [13] LOMMEN A,GODEJOHANN M,VENEMA D P,HOLLMANP C H,SPRAUL M. Application of directly coupled HPLC-NMR-MS to the identification and confirmation of quercetin gly-cosides and phloretin glycosides in apple peel[J]. AnalyticalChemistry,2000,72(8):1793-1797.
- [14] SCALBERT A,WILLIAMSON G. Dietary intake and bioavail-ability of polyphenols[J]. Journal of Nutrition,2000,130(8SSuppl):2073S.
- [15] PARK Y S,KIM B W,KIM T C,JANG H G,CHON S U,CHOJ Y,JIANG S H,HEO B G. Physiological activity of methanolextracts from Korean kiwifruits[J]. Korean Journal of Horticul-tural Science and Technology,2008,26(4):495-500.
- [16]李琛,张婷,罗安伟,焦应申,唐妙玲,陈翰,姚婕.8种猕猴桃抗氧化活性评价及基于HPLC与FT-IR指纹分析的品种区分[J].现代食品科技,2016,32(6):288-297.LI Chen,ZHANG Ting,LUO Anwei,JIAO Yingshen,TANGMiaoling,CHEN Han,YAO Jie. Evaluating antioxidant capacityof eight kiwifruit cultivars and identifying cultivars based onHPLC and FT-IR fingerprint analysis[J]. Modern Food Scienceand Technology,2016,32(6):288-297.
- [17] DU G R,LI M J,MA F W,LIANG D. Antioxidant capacity andthe relationship with polyphenol and Vitamin C in Actinidiafruits[J]. Food Chemistry,2009,113(2):557-562.
- [18] BURSAL E,GüLCIN I. Polyphenol contents and in vitro antiox-idant activities of lyophilised aqueous extract of kiwifruit(Actin-idia deliciosa)[J]. Food Research International,2011,44(5):1482-1489.
- [19] ZUO L L,WANG Z Y,FAN Z L,TIAN S Q,LIU J R. Evalua-tion of antioxidant and antiproliferative properties of three Actin-idia(Actinidia kolomikta,Actinidia arguta,Actinidia chinensis)extracts in vitro[J]. International Journal of Molecular Sciences,2012,13(5):5506-5518.
- [20]焦中高,胡丽娜,张春岭,刘慧,杨文博,吕真真,王思新,刘杰超.采后UV-C处理对软枣猕猴桃果实酚类物质及抗氧化活性的影响[J].现代食品科技,2016,32(11):177-183.JIAO Zhonggao,HU Lina,ZHANG Chunling,LIU Hui,YANGWenbo,LüZhenzhen,WANG Sixin,LIU Jiechao. Effect of Ul-traviolet-C irradiation on phenolic compounds and antioxidantactivity of postharvest Actinidia arguta fruit[J]. Modern FoodScience and Technology,2016,32(11):177-183.
- [21]徐小彪,黄春辉,曲雪艳,陈明,钟敏,郎彬彬,陈楚佳,谢敏,张文标.毛花猕猴桃新品种‘赣猕6号’[J].园艺学报,2015,42(12):2539-2540.XU Xiaobiao,HUANG Chunhui,QU Xueyan,CHEN Ming,ZHONG Min,LANG Binbin,CHEN Chujia,XIE Min,ZHANGWenbiao. A new easy peeling Actinidia eriantha cultivar‘Gan-mi 6’[J]. Acta Horticulturae Sinica,2015,42(12):2539-2540.
- [22]陈楚佳,黄春辉,陶俊杰,曲雪艳,钟敏,吴寒,徐小彪.毛花猕猴桃‘赣猕6号’果实发育期间色素变化及呈色分析[J].果树学报,2016,33(11):1424-1430.CHEN Chujia,HUANG Chunhui,TAO Junjie,QU Xueyan,ZHONG Min,WU Han,XU Xiaobiao. Changes of pigment con-tents and flesh color generation analysis during fruit develop-ment of‘Ganmi 6’(Actinidia eriantha)[J]. Journal of Fruit Sci-ence,2016,33(11):1424-1430.
- [23] BONILLA E P,AKOH C C,SELLAPPAN S,KREWER G. Phe-nolics content and antioxidant capacity of Muscadine grapes[J].Journal of Agricultural Food Chemistry,2003,51(18):5497-5503.
- [24] JIA Z S,TANG M C,WU J M. The determination of flavonoidcontents in mulberry and their scavenging effects on superoxideradicals[J]. Food Chemistry,1999,64(4):555-559.
- [25] BENZIE I F F,STRAIN J J. The ferric reducing ability of plas-ma(FRAP)as a measure of‘antioxidant power’:the FRAP as-say[J]. Analytical Biochemistry,1996,239:70-76.
- [26]韦献雅,殷丽琴,钟成,章明海,牛应泽. DPPH法评价抗氧化活性研究进展[J].食品科学,2014,35(9):317-322.WEI Xianya,YIN Liqin,ZHONG Cheng,ZHANG Minghai,NIU Yingze. Advances in the DPPH radical scavenging assayfor anti-oxidant activity evaluation[J]. Food Science,2014,35(9):317-322.
- [27]谭琳,郑晓燕,王甲水,马伏宁,康由发,臧小平,马蔚红.大果榕果实乙醇提取物抗氧化活性及对α-葡萄糖苷酶和乙酰胆碱酯酶抑制活性[J].食品科学,2016,37(13):77-81.TAN Lin,ZHENG Xiaoyan,WANG Jiashui,MA Funing,KANG Youfa,ZANG Xiaoping,MA Weihong. Antioxidative ac-tivity and inhibition ofα-glucosidase and acetylcholinesteraseof Ficus macrophylla fruit ethanol extract[J]. Food Science,2016,37(13):77-81.
- [28]郭雪峰,岳永德,汤锋,王进,姚曦.用清除超氧阴离子自由基法评价竹叶提取物抗氧化能力[J].光谱学与光谱分析,2008,28(8):1823-1826.GUO Xuefeng,YUE Yongde,TANG Feng,WANG Jin,YAOXi. Detection of antioxidative capacity of bamboo leaf extractby scavaenging superoxide anion free radical[J]. Spectroscopyand Spectral Analysis,2008,28(8):1823-1826.
- [29] ERCISLI S,AKBULUT M,OZDEMIR O,SENGUL M,ORHAN E. Phenolic and antioxidant diversity among persim-mon(Diospyrus kaki L.)genotypes in Turkey[J]. InternationalJournal of Food Sciences&Nutrition,2008,59(6):477-482.
- [30] LI P M,DU G R,MA F W. Phenolics concentration and antioxi-dant capacity of different fruit tissues of astringent versus non-astringent persimmons[J]. Scientia Horticulturae,2011,129(4):710-714.
- [31]蒲飞.柿果实酚类物质含量、生物活性及其相关酶的研究[D].杨凌:西北农林科技大学,2014.PU Fei. Studies on phenolic substance content,biological activi-ty and related enzymes in persimmon[D]. Yangling:NorthwestA&F University,2014.
- [32]刘杰超,张春岭,陈大磊,焦中高.不同品种枣果实发育过程中多酚类物质、VC含量的变化及其抗氧化活性[J].食品科学,2015,36(17):94-98.LIU Jiechao,ZHANG Chunling,CHEN Dalei,JIAO Zhonggao.Changes of polyphenols during fruit development and their anti-oxidant activities in different varieties of jujube(Zizyphus juju-ba L.)[J]. Food Science,2015,36(17):94-98.
- [33]夏乐晗,陈玉玲,冯义彬,焦中高,刘慧,王其海.不同品种杏果实发育过程中类黄酮、总酚和三萜酸含量及抗氧化性研究[J].果树学报,2016,33(4):425-435.XIA Lehan,CHEN Yuling,FENG Yibin,JIAO Zhonggao,LIUHui,WANG Qihai. Studies on the contents of flavonoids,totalPhenol and triterpene acid and their antioxidant activity duringfruit development of different varieties of apricot[J]. Journal ofFruit Science,2016,33(4):425-435.
- [34] TRIPOLI E,GUARDIA M L,GIAMMANCO S,MAJO D D,GIAMMANCO M. Citrus,flavonoids:molecular structure,bio-logical activity and nutritional properties:a review[J]. FoodChemistry,2007,104(2):466-479.
- [35]徐贵华,胡玉霞,叶兴乾,刘东红.椪柑、温州蜜桔果皮中酚类物质组成及抗氧化能力研究[J].食品科学,2007,28(11):171-175.XU Guihua,HU Yuxia,YE Xingqian,LIU Donghong. Composi-tions of phenolic compounds and antioxidant capacity in Ponkan(Citrus poonensis Hort. ex Tanaka)and Satsuma Mandarin(Cit-rus unshiu Marc)peels[J]. Food Science,2007,28(11):171-175.
- [36]王晓宇,杜国荣,李华.抗氧化能力的体外测定方法研究进展[J].食品与生物技术学报,2012,31(3):247-251.WANG Xiaoyu,DU Guorong,LI Hua. Progress of analyticalmethods for antioxidant capacity in vitro[J]. Journal of Food Sci-ence and Biotechnology,2012,31(3):247-251.
- [37] MA X W,WU H X,LIU L Q,YAO Q S,WANG S B,ZHAN RL,XING S S,ZHOU Y G. Polyphenolic compounds and antioxi-dant properties in mango fruits[J]. Scientia Horticulturae,2011,129(1):102-107.
- [38] MEYER A S,YI O S,PEARSON D A,WATERHOUSE A L,FRANKEL E N. Inhibition of human low-density lipoprotein ox-idation in relation to composition of phenolic antioxidants ingrapes(Vitis vinifera)[J]. Journal of Agricultural&Food Chem-istry,1997,45(5):1638-1643.
- [39] WELIOGLU Y S,MAZZA G,GAO L,OOMAH B D. Antioxi-dant activity and total phenolics in selected fruits,vegetables,and grain products[J]. Journal of Agricultural&Food Chemis-try,1998,46(10):4113-4117.
- [40] BOCCO A,CUVELIER M E,RICHARD H,BERSET C. Anti-oxidant activity and phenolic composition of citrus peel andseed extracts[J]. Journal of Agricultural&Food Chemistry,1998,46(6):2123-2129.